CAZyme Information: MGYG000002609_01493

Basic Information

• Species: CAG-882 sp003486385
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-882; CAG-882 sp003486385
• CAZyme ID: MGYG000002609_01493
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1216		137343.05	4.8072

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002609	3323522	MAG	China	Asia

• Gene Location: Start: 16000; End: 19650 Strand: +

Full Sequence      

MKRKTCLNNGWQFLEKEYSEENLQCPDGEYMPVRLPHDYLIYDVGNLYRDSVGWYRRKLM
YVPDGLERVLFFEGIYMDSEVFVNGRSAGTWKYGYSSFEVDITPYLEAGENELLVRVVHR
SPNSRWYSGAGIYRSVYLIERERTHIASSGLYITPVKREASDTWDVEVEVEVAQGADDEK
PFDSIRYIITQKNGKHDMRSNEEDSLCIIFNQLIGEKSNIYRHTFEITAPKLWDIYCGNL
YELRAELIKAGEVFDYEQSVFGFRTIEIDSDKGFLLNGRHVKIKGSCEHHGFGCLGAAVN
KSAVRRKLEGLKRMGVNAIRTAHNMPSVEFMELADEMGFLVDSEAFDMWGHTKTKYDYGR
FFAESAKKDVESWIRRDRNHPSIIMWSVGNEIYDMHAGDEGLVELVMLLGEVKKHDPKKH
AFTTFASNYMRWENTQKCAGLVDAVGYNYGEYLYKEHHAKHPDWVIYGSETSSMLASRGV
YHFPIETSTLCDTDEQCSALGNCSAGWGAHSYTDNIIADRDAGFSLGQFIWAGVDYFGEP
TPYHTRNSYFGQMDTAGFEKDSYYIYQAEWTSFKENPMVHVFPHWDFNPGQLVDVCVCSN
APIVELFVNGMSMGRKVIDHAHGTELVPHWKVPYEKGYVEAVAYDEAGNELARQMRHSFS
DADRLVIEADRRQMENRYGELIFVEIGALDKDGYTVDNANNRVEIKVTGAGRLVGLDNGD
STDFDQVKTSSRRLFGGKLLAVIAPVEGVTGKITVTAEAEGLWAASIDLCVTDDKNMRDE
YLVQSCICTDKNNMAEDMSENSFQLSEDAEKRNHTRNAEGLMDSVYSENEIPIRKVELTA
ETFTLSADKSSVKVHWKLYPENATYRDLKWSITDTTGIAIKSATVIQQGEADEAMIKTGT
GKAPDKMEAAEAQVKMEAVDMLSEGYVTVNAAGDGSFYLRCDAYNGRTAAGVKSSLEFTA
AGIGPAYLDPYEPVSAGLCLERPHGLSEGVGHGVRFLGKEKTKISFGAMDFGVNGSEQLQ
MYLFKYYPGSVKLRIYLDDDSKNILDAEFDESAGWLEFKKAEYRLSERIKGIHRISIESE
DNFQLNSFSFVPVLHGFDRINAADYDEIFGDSYKLDGTAVTGIGNNVSIIYRHLNMGAHS
ADKIKICGRTANARDSIRLKIDCAGTERTELIEFEESAAYVEHEFNIEPVGGEITVTLLY
LPGCNFDLEWFRFLQE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	577	1e-104	0.5944148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.15e-61	47	586	77	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.35e-40	6	453	14	477	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.11e-32	53	391	111	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam18565	Glyco_hydro2_C5	2.32e-30	665	767	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK09525	lacZ	4.13e-25	52	391	121	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANS74671.1	0.0	1	1214	1	1154
QUL53148.1	0.0	7	1214	7	1159
AIQ59925.1	0.0	1	1216	1	1161
AIQ48506.1	0.0	7	1216	7	1174
AIQ31155.1	0.0	1	1216	1	1162

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	2.97e-150	3	772	2	802	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	5.05e-150	3	762	8	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	2.08e-141	3	760	14	833	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	5.65e-141	3	760	14	833	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5T98_A	7.39e-128	7	765	30	814	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	9.46e-138	27	769	68	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	6.27e-95	5	761	49	811	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	6.26e-55	7	643	56	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.76e-32	7	715	19	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	4.80e-27	7	769	31	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000108	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002609_01493.