CAZyme Information: MGYG000002613_00959

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000002613_00959
• CAZy Family: GH78
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
726	MGYG000002613_37|CGC1	83195.79	6.8787

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002613	2968338	MAG	China	Asia

• Gene Location: Start: 7129; End: 9309 Strand: -

Full Sequence      

MKRFYFYILSLLLSGTAMAQTWIWFPGDMDIWTGNRLNTLRTEGGGMWPAFWRTDSHWQS
VEFSKTVELDKEEDVEIAAEGQYSVKLDQWFLFGMPKRITVPAGKHTLRISVSNLVSPPA
LWVNGNTLKSDDTWTVSNYAKAERPDTWSDMNGKPVFDSKEKRPSQYKLPTTAVSAVASK
QEGREMFVDFGKESMGYLRLDGVSGNGQVKIWYGESPDEARDKKWAYLQDRVQFLADSVV
DLQSMTVSPRSANNYTLARSRAMRYALVEWEDGMNIDNLTLMSEMKDLGDGYRGSFLCSD
TLLNKIWQVGAYTLHLTNREVMIEGIKRDRWMWSGDAIQSYLMNYYVFMDAPAVKRTIWA
LRGKDPVEQHINTILDYTFYWFNSVYDYYLYTADEDFVHRIYPRMQSLMAFVESRLNGRG
MVEGKKGDWVFVDWSPGSMSKGGELSFEQMVYLRSLDAMSLCAGIVGNDADKQKYSGRAE
KLRSQLVPLFWNDSKKAFVHTFENGEQSAQVTRYANMFAVLYGLVDQQKGQEILHSVLLN
DSIMQITTPYMRFYELEALCALGEHQRVLKEMRSYWGGMLKEGATSFWETYDPNETYPER
LGMYGHPYGKSLCHAWGASPLYLLGKYFLGVRPVKPGYAEWEACPQLGDLKWMKGDVPTP
NGNIHIEMTRKEIIVRSEGCGNGTVYFKSAKRPKASSVMLKAIGNNTYQMLVEPGKEVKV
AFSFRD

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	181	672	8.2e-107	0.9523809523809523

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.46e-22	294	627	4	339	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam06202	GDE_C	7.75e-07	372	534	81	256	Amylo-alpha-1,6-glucosidase. This family includes human glycogen branching enzyme AGL. This enzyme contains a number of distinct catalytic activities. It has been shown for the yeast homolog GDB1 that mutations in this region disrupt the enzymes Amylo-alpha-1,6-glucosidase (EC:3.2.1.33).
pfam17390	Bac_rhamnosid_C	0.003	630	676	1	48	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam04685	DUF608	0.003	385	492	108	212	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT92124.1	9.81e-283	2	717	3	727
ALJ62146.1	7.97e-282	2	717	3	727
QIU95851.1	2.05e-279	1	722	1	732
QUT58576.1	4.09e-279	10	717	11	709
ABR40300.1	4.09e-279	10	717	11	709

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CIH_A	2.33e-258	10	722	8	729	Crystalstructure of a putative alpha-rhamnosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2OKX_A	1.16e-44	293	669	536	916	Crystalstructure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1],2OKX_B Crystal structure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1]
4XHC_A	2.61e-29	170	629	77	543	ChainA, Alpha-L-rhamnosidase [Klebsiella oxytoca],4XHC_B Chain B, Alpha-L-rhamnosidase [Klebsiella oxytoca]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000677	0.998504	0.000220	0.000208	0.000176	0.000166

TMHMM  Annotations     

start	end
5	24