CAZyme Information: MGYG000002624_00263

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; Borkfalkia
• CAZyme ID: MGYG000002624_00263
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1483		166331.58	4.0549

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002624	1428680	MAG	China	Asia

• Gene Location: Start: 121530; End: 125981 Strand: -

Full Sequence      

MQLKKTFRLTAVLIVLLLAISLLVVACSKGGDDNGGDTPATENAFELIDNDRAALIVVEG
TNEPGATDGDYPGVVRAAYDLQEDVETVTGVKPVVAQSVTGYADYAIIIGTIGKSTAIDS
MVESGKIDVSQVEGKWETYLIETVDDPFGDGKVDTALVIAGSDKRGSIYGIYYISEMLGV
SPWTFFADSVPAHKDNLTLAADYRYVSEEPDVQYRGIFINDEEDLELWGRVMDDGKHMGP
KLYEEIFEMLLRSKANFLWPAMHACSDAFVGYVENPINADYYGIVIGTSHCDMLLRNNLN
EWDNFVAQYKQEHPEYANVKVKYDYTHVESQPVIEEYWRESVRANKDYEVAWNLGMRGSH
DEGFNTEYINSEPWYGDKCKLLEDIIDLQQNILKEELGLDSLDGVFMTFQPYKEVQELYN
NGLDLPENITIIWADDNHGFIRNVPNAEERERSGGNGIYYHASYWGPDNESYMWINSMPQ
TMVYEELSKAYKSGIQKAWVLNSGDTQMNMLEIDFFADLGYDIDQYNNENVFEQYYGKVA
AREYGEEFADDIIEIMKDYTQMTNARKLEHMSVDLFSDAYGDEMERRASEYKDLWERAEA
VYEAIPETQQNTFYETILFEVRCAYYINAEFYYAHKGNVAYEQGRNATAWNCYNYSLDFN
RMRKDEISYYNNILADGKWENIMDPENYVPPVMSGFANGGPTFTLGATEQGVIVEGESAE
QSSSSLAFYNYAQGRKYIDVFNKGAGNFSFSVSADKDFVQLSTTQGIVTDEQRVWVSIDW
SKVTGNDTATVTITSGSYTKTVTINVNYANYDLGESTYVEQDGYVSIEAEHYSASHETGA
ASWEVIKDLGRVSGDMVRAESNTLVSYNEDTFATSAPYLEYNVYFTSAGTFEMEVYRLPT
LHSQGHVRLAVGVGSGTPTIIEGERDYGTNNPAWEEGVFTQIIKHTITIDIAQPGLTTIR
LYMIDPWICVDKMVIYTAEKQDSYFGPLESYNTTYNTDPGANAYYDAFYGTSGETYVLPE
DYNIEQTYGTGYFVEQDGVLAIETETAVLGDDQYASADGWVVAQTDEGIAMRTKEERKEY
GATMDSAPRMDYEIIITTPGTYTVWVNIQSPNPRSSSYGIGINGVPRYTKGEYAYDREEF
FHWREGNSITFSAAGRYTLNVYCVQDGLAIDKIYLTRGNETPTNATFVQSARTTIWKGSN
LDAVKADAALRSDLAAALDAVGNYYAIPTGTGLGQYEEADVTALKAAVEAGYEMLNSTSA
LVEGNVTETISDLTDALAAMNAGRNMSGDGVNYLVYETYEDSIVGLDPYGYEIHSIDRTP
DIIVREADDGSKYLNVRTFKNQRLQQEAMLQYSFAEQTGTFTVEARMSFNEAVWGNAVYL
VNEDGNYAVSVAFEYSYGNYNLVAYNANVKTVLASYNRNEMVDIRVDVDVDSDRFDVYVN
DELAGENFSFRNRADSIGAVVFGSSVRDADLRVDSLAVYTTAE

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	45	687	8.1e-185	0.8206599713055954

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.73e-119	209	570	2	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	5.05e-47	817	989	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam17829	GH115_C	2.66e-10	1033	1179	2	162	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	4.26e-05	51	172	19	118	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).
cd14948	BACON	0.009	741	805	11	81	Bacteroidetes-Associated Carbohydrate-binding (putative) Often N-terminal (BACON) domain. The BACON domain is found in diverse domain architectures and accociated with a wide variety of domains, including carbohydrate-active enzymes and proteases. It was named for its suggested function of carbohydrate binding; the latter was inferred from domain architectures, sequence conservation, and phyletic distribution. However, recent experimental data suggest that its primary function in Bacteroides ovatus endo-xyloglucanase BoGH5A is to distance the catalytic module from the cell surface and confer additional mobility to the catalytic domain for attack of the polysaccharide. No evidence for a direct role in carbohydrate binding could be found in that case. The large majority of BACON domains are found in Bacteroidetes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASA21655.1	9.07e-274	2	1456	1	1473
AFO77172.1	2.71e-201	40	1000	45	983
ADJ45460.1	2.71e-201	40	1000	45	983
AGT84300.1	2.71e-201	40	1000	45	983
AEK42229.1	2.71e-201	40	1000	45	983

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	5.82e-129	47	990	16	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	2.10e-98	73	991	31	964	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
5BY3_A	6.66e-87	66	779	31	714	Anovel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans [Bacteroides thetaiotaomicron VPI-5482]
7PUG_A	2.43e-80	33	683	3	625	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	4.35e-80	33	683	2	624	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000002	1.000078	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
7	26