CAZyme Information: MGYG000002624_00857

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; Borkfalkia
• CAZyme ID: MGYG000002624_00857
• CAZy Family: GT2
• CAZyme Description: Glucans biosynthesis glucosyltransferase H

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
562	MGYG000002624_9|CGC1	64747.96	9.8153

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002624	1428680	MAG	China	Asia

• Gene Location: Start: 60254; End: 61942 Strand: -

Full Sequence      

MKDKRKGAGGRKSPVLYIVMLSVWSLLCAAIWYYFVPAFITPPFAAGTGGNLALRIFAVV
LLVLNALFISYFWLNGVKDFLYVMWYTFSRRKLEAKYRKVLETDVRSVSDKVLMLYCTCN
DFDGESLRRSMRQTYAHAETVILDDSSQPEYKEKVDAFARETGVRVVRRADRKGFKAGNI
NHFLQSEEVRRSDYGYVVILDSDEIIPPDFIVSCLRYFYAYENVGIVQANHVATRNRNFF
MKLFHIGVNSHWPTYQTMKHNYGFSSMLGHGAMIRRECYEQAGGFPDLVAEDLCLSIEMR
NKGYVVAFAPDIVCEEEYPVDYAAFKKRHSKWTQGNLEFIKKYTGKIFRSRMRWFEKLDI
ILFTYNLPLTAIFAFYIILNIVILPVIGVDVGRVYPLWMIVPTVVFFFSPMLNDVFTWLF
RINLFRFLFYLLCVVVLYGSMLFTSLVSALLGMFGKKAKFIVTPKDSQKMGFLCALRIQW
KELLFSTVLVAVSMVFVRTFLPVVLIAVTGYLSFALLFFANKKYSAEQTRRIDARTFAVS
AKQNGLYFSGSAFGRAKSAPEH

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	124	333	8.2e-25	0.9130434782608695

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06421	CESA_CelA_like	1.04e-35	126	338	20	232	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
COG1215	BcsA	5.78e-30	52	462	4	416	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06437	CESA_CaSu_A2	6.36e-27	142	335	37	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.
cd06423	CESA_like	2.52e-21	124	292	13	180	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
cd06435	CESA_NdvC_like	4.63e-20	125	343	16	235	NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. Bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans during growth under hypoosmotic conditions. Two genes (ndvB, ndvC) are involved in the beta-(1, 3), beta-(1,6)-glucan synthesis. The ndvC mutant strain resulted in synthesis of altered cyclic beta-glucans composed almost entirely of beta-(1, 3)-glycosyl linkages. The periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans function for osmoregulation. The ndvC mutation also affects the ability of the bacteria to establish a successful symbiotic interaction with host plant. Thus, the beta-glucans may function as suppressors of a host defense response.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEU60418.1	1.08e-202	12	547	23	565
QWW19438.1	1.99e-181	11	528	5	507
CAB51329.1	1.46e-167	11	521	5	504
AJQ91551.1	1.15e-148	12	506	3	478
CAO99133.1	3.27e-148	12	506	3	478

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9T0L2	1.08e-14	142	351	147	362	Probable glucomannan 4-beta-mannosyltransferase 15 OS=Arabidopsis thaliana OX=3702 GN=CSLA15 PE=3 SV=2
Q84W54	3.47e-14	142	361	160	386	Probable glucomannan 4-beta-mannosyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CSLA1 PE=2 SV=1
Q7PC73	8.42e-14	142	342	170	376	Probable glucomannan 4-beta-mannosyltransferase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CSLA5 PE=2 SV=1
Q7XIF5	1.42e-12	142	342	185	391	Probable glucomannan 4-beta-mannosyltransferase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CSLA7 PE=2 SV=1
Q9FA52	3.33e-12	168	338	163	343	Glucans biosynthesis glucosyltransferase H OS=Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=opgH PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000004	0.000004	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
13	35
55	77
361	383
398	420
427	449
499	521