CAZyme Information: MGYG000002643_01305

Basic Information

• Species: Turicimonas sp900542195
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Turicimonas; Turicimonas sp900542195
• CAZyme ID: MGYG000002643_01305
• CAZy Family: GT2
• CAZyme Description: Cellulose synthase catalytic subunit [UDP-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
886	MGYG000002643_17|CGC1	100865.63	9.6782

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002643	2740903	MAG	China	Asia

• Gene Location: Start: 22179; End: 24839 Strand: -

Full Sequence      

MLLTQLFRSFDLSLKTDLVMLKSHCCSSWSYVCNAFFFLIAWAVFPLENKLYKKIFRNRA
FYFPQINFHSLHPLDAFRILIQLGFLCLFVITKDKFSQRKAKGRPGTLSQFVRYVRVNIG
REIRNLSETLSKLLLSDSSSAAEKGKNSAEEPPPHKMGWVRKTVIGLLLSVGLVLYFLCI
TQPFSLEEQIIFLSILWVSALLLFQAQTRLTLLMLIVISVIVSSRYIWWRYTETINPNSF
MSVIFSWMLILAETYAFIVMLLGYFQVCWALDRKPTSMPKDPSLWPHVDIFIPTYNEPLD
VVKPTVYACLTMDWPKEKLHVHVLDDGSRPEFEEFSNSVGAGYIKREKHNHAKAGNINHA
LTKTSGDFITIFDCDHVPVRSFLKKTMGWFFKDKNIALIQTPHHFYSQDPFEKNLHLSEH
VPNENSLFHDFIQKGNDTWNATMFCGSCAIMRRKALEEVGGIAVETVTEDAHTSLKLNRK
GWSSAFLSTPLSAGLSTETLAAHIGQRIRWARGMIQIFRLDNPLFGKGLTIPQRLCFLNA
MIHFLHGLPRIIFLLAPLPFLFANIYVIYASAIAIFVYLVPHMVHSTMTTYVIHKGYRFP
FVSAIYETILSWYIFVPTLVAIFAPHKGKFNVTAKGGVIDKAYVDWSVAKPYLYLLILNL
AGLCFGLYKMIGASQYQVLMLAINIGWILYNLIILFASIAVCVESVQQRKFPRVAAMQKV
TVELSDGHKVKAVLAAFSQKDCQLVFKIPSAIPNFKLGESVKLIFGEGKTIHEFNCTVSA
IFSAGVIDLDVHQETLQKEKEYTACTFGVPELWINKQAKIKGFGMLDGFMALLRMARQGV
EAFVHYTPPSSSWLFYITVGSLKWIISFIPRVPKLRSQSKQTVFAS

Enzyme Prediction     

EC	2.4.1.12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	289	460	3.4e-37	0.9941176470588236

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR03030	CelA	0.0	176	846	21	712	cellulose synthase catalytic subunit (UDP-forming). Cellulose synthase catalyzes the beta-1,4 polymerization of glucose residues in the formation of cellulose. In bacteria, the substrate is UDP-glucose. The synthase consists of two subunits (or domains in the frequent cases where it is encoded as a single polypeptide), the catalytic domain modelled here and the regulatory domain (pfam03170). The regulatory domain binds the allosteric activator cyclic di-GMP. The protein is membrane-associated and probably assembles into multimers such that the individual cellulose strands can self-assemble into multi-strand fibrils.
PRK11498	bcsA	0.0	24	881	14	851	cellulose synthase catalytic subunit; Provisional
cd06421	CESA_CelA_like	3.42e-120	286	518	1	234	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
COG1215	BcsA	2.33e-57	243	698	10	430	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06437	CESA_CaSu_A2	1.45e-33	286	513	1	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQQ96025.1	0.0	1	886	1	886
ANU67170.1	0.0	1	886	1	886
QQS88758.1	1.38e-294	30	873	29	860
QDA53875.1	6.11e-287	34	874	33	874
QDA53780.1	3.30e-270	164	873	61	766

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LBY_A	3.93e-255	22	881	25	863	ChainA, Cellulose synthase catalytic subunit [UDP-forming] [Escherichia coli K-12]
5EJ1_A	9.58e-99	225	836	67	708	ChainA, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
4HG6_A	5.18e-98	225	836	79	720	ChainA, Cellulose Synthase Subunit A [Cereibacter sphaeroides]
4P00_A	5.29e-98	225	836	80	721	ChainA, Cellulose Synthase A subunit [Cereibacter sphaeroides 2.4.1],4P02_A Chain A, Cellulose Synthase subunit A [Cereibacter sphaeroides 2.4.1],5EIY_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1],5EJZ_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37653	1.20e-255	22	881	25	863	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli (strain K12) OX=83333 GN=bcsA PE=1 SV=3
Q8Z291	1.28e-255	22	874	25	857	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhi OX=90370 GN=bcsA PE=3 SV=1
Q8X5L7	2.39e-255	22	881	25	863	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli O157:H7 OX=83334 GN=bcsA PE=3 SV=2
Q93IN2	7.24e-255	22	874	25	857	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bcsA PE=3 SV=1
P58931	3.16e-206	154	834	33	699	Cellulose synthase catalytic subunit [UDP-forming] OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=bcsA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999974	0.000077	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
25	47
74	91
164	186
190	204
211	228
243	265
535	554
558	580
601	623
651	668
681	703