CAZyme Information: MGYG000002657_00202

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-95
• CAZyme ID: MGYG000002657_00202
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
822	MGYG000002657_2|CGC1	94688.55	4.5923

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002657	2838774	MAG	United Republic of Tanzania	Africa

• Gene Location: Start: 78063; End: 80531 Strand: +

Full Sequence      

MGEVFSKEQLYDRTPRVYPRDASEVRFLLGGIGTGNISVNPRGKLLDWEIFNWPSKNTKF
PLAFFAIRTENECMTQPVAKILESRLLPPYTSSHGYLQAELVNLPRMEDSEMVCEYPFAR
VQFKDSELPVQVSMESYTPFIPLNTDDSSIPCAIIRYKVRNTTEYPTKVSLVGTLPNASG
FEGYDVIENLILADSVKNEFREYKDVKGLYYSCEHLDENHIRYGNMAILTKGENITYKTQ
WFDGEWVDGIQDFWDDFTEDGILETETVSDSVGCEFAQFHNFSFLKRREKIGSIGSYKEL
APGEETVFEFVITWYFPNRVKAWIEFDEDYENFRQGKYGTVRNYYATKFQDAWQVGTYVY
DHMERLEKGSRDFSDAMFHRTNLPYYVIDALTANITNLRSNLCFRLEDGTFGGFEGIRDY
IGCGYGSVPHVWNYAQTVAFLFPDLEQSMRRVEFLRETDENGCMSTRMFSVFDQERYAMV
PACDGQLGSVVRIYRDFKNTGDVTFLKELWPKAVAAMDYAFKQWDLDGDDVLDGQQNTTY
DIEFYGPNPMTDSIFLAALRCCEEMAAILGDEDHQKQYADAYRQGCERADKLMFNGEYYK
QVEKEPDKYKYQFGEGCLSDQLLGQFLAYMAGIGEILPHEHLRSAMEAVFRYNYKMDFYH
TDSVHRAYAINEEHGVVVATWPRGGRPKFPLSYAGEVWTGVEYEVAVNLIYLGCLEEGLT
IVKAVRDRYDGYKRNPFSEIESGHHYCRAMASWGVLNALLGLKSDMYQKTLSIHPVVQEN
DISSFFICGKAWGVYSQKMVDGKLEKTIDVLYGSLDDVTVTD

Enzyme Prediction     

No EC number prediction in MGYG000002657_00202.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	428	755	1.6e-70	0.9476584022038568

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12215	Glyco_hydr_116N	1.16e-68	27	366	2	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
COG4354	COG4354	4.78e-57	29	761	17	719	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam04685	DUF608	9.53e-51	409	755	1	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02725.1	0.0	1	822	1	821
QHW30807.1	2.66e-295	4	820	6	811
AZN42723.1	1.80e-279	3	813	12	812
ALS26632.1	7.77e-265	16	820	15	809
AUA17208.1	3.32e-260	10	815	10	813

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	1.69e-35	29	755	56	780	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	1.76e-35	29	755	59	783	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
5BVU_A	1.77e-35	29	755	60	784	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
7W2V_A	2.03e-35	29	755	103	827	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2W_A	2.03e-35	29	755	103	827	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11],7W2X_A Chain A, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9HCG7	2.34e-23	29	718	154	841	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q5M868	7.57e-21	5	789	117	907	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q69ZF3	6.89e-20	29	718	145	832	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q7KT91	1.21e-09	116	717	227	890	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999976	0.000055	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002657_00202.