CAZyme Information: MGYG000002668_00822

Basic Information

• Species: Prevotella sp900552965
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900552965
• CAZyme ID: MGYG000002668_00822
• CAZy Family: GH63
• CAZyme Description: Glucosidase YgjK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
627	MGYG000002668_76|CGC1	71370.58	5.9175

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002668	2820424	MAG	United Republic of Tanzania	Africa

• Gene Location: Start: 441; End: 2324 Strand: -

Full Sequence      

MNYKSIAIMTLGLAMLSLDSVAQTTRGAYKDIVDISLTPGVPRRQNGCFTDMGSWMGFTL
SEKEKPTTGFCGPFSIYYRNWYARSLVSLSGASLAEQSYYPGEVRMTLRKDGAEIMESMQ
FVDARTALLTVSNPAKLPLTFTGDSISSKLHLSLKGNTVTIGDLHSERIMLTFPKQVKLE
TSDHNYVAQLPAGVSQLTVAISIVEQDTEESVQNVHIASLLANPSAALEANESRWNGYLE
KVIRDDMPADYNRVAAKSIVTLLSNWRTKRGNLYHDGIVPSHAVGYFVGCWAWDCWRFSA
GMASFFPELAKDNIRVMFDYQQPDGMIIDCIYPDASENNTRDSKPPLAAWAVNEIYEHNN
DLAFVKEMYPKLLKYHKWWYEKRDHDKNHICEFGSTDGTLEAAAWESGMDNAIRFDDTKM
LKNDAENAWSTDQESVDLNAYLSLEYTLLKKFAELLGEPFDLPDYRNLVADYFFDKKDGF
FYDRRLNENRDFVREAGCEGYIPFWANIATPKQFAMARKLFDNKKKFSTYIPFPTIAADN
PKYDPKGYWRGPIWLDQTYYGIKGYRNYGEHRKADAYTDQVFRNLEGISAGTPIHENYDT
HTGKPLKASHFSWSAACLLMLYNDYGK

Enzyme Prediction     

No EC number prediction in MGYG000002668_00822.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	175	625	2.6e-108	0.5789473684210527

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10137	PRK10137	3.06e-134	4	625	3	783	alpha-glucosidase; Provisional
COG3408	GDB1	5.38e-19	276	622	270	603	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
pfam01204	Trehalase	1.09e-06	343	527	178	402	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.
pfam03200	Glyco_hydro_63	3.00e-06	362	621	185	492	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.
PLN02567	PLN02567	4.68e-04	343	511	203	422	alpha,alpha-trehalase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNT67704.1	0.0	1	627	1	626
QUT89313.1	1.43e-255	26	627	32	642
QJR62245.1	1.17e-252	26	627	34	644
QJR74523.1	1.17e-252	26	627	34	644
QJR66502.1	1.17e-252	26	627	34	644

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W7S_A	7.75e-78	49	625	32	756	Escherichiacoli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7S_B Escherichia coli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7T_A Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7T_B Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7U_A Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12],3W7U_B Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12]
3W7X_A	4.02e-77	49	625	32	756	Crystalstructure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],3W7X_B Crystal structure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],5CA3_A Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5CA3_B Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3W7W_A	5.59e-77	49	625	32	756	Crystalstructure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],3W7W_B Crystal structure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],5GW7_A Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5GW7_B Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3D3I_A	1.51e-74	49	625	33	757	Crystalstructural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12],3D3I_B Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12]
7PQQ_B	2.47e-33	402	625	42	305	ChainB, Anti-RON nanobody,Megabody 91,Glucosidase YgjK [Lama glama]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P42592	6.40e-77	49	625	55	779	Glucosidase YgjK OS=Escherichia coli (strain K12) OX=83333 GN=ygjK PE=1 SV=1
D8QTR2	2.32e-19	283	622	90	486	Mannosylglycerate hydrolase MGH1 OS=Selaginella moellendorffii OX=88036 GN=MGH PE=1 SV=1
D8T3S4	4.11e-19	283	624	90	488	Mannosylglycerate hydrolase MGH2 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_447962 PE=3 SV=1
K5BDL0	5.36e-08	291	619	40	442	Glucosylglycerate hydrolase OS=Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849) OX=1122247 GN=ggh PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000239	0.999098	0.000175	0.000174	0.000153	0.000145

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002668_00822.