CAZyme Information: MGYG000002669_00629

Basic Information

• Lineage: Bacteria; Eremiobacterota; Xenobia; Xenobiales; Xenobiaceae; Bruticola
• CAZyme ID: MGYG000002669_00629
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase C

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
219		24612.04	4.5677

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002669	1762651	MAG	United Republic of Tanzania	Africa

• Gene Location: Start: 3011; End: 3670 Strand: -

Full Sequence      

MDMDSEAVKRRDKNPSDSCSFLSVLVKLLFVFVAIFSVLYVNWSRLERYCYPLKYENTVI
AAAGEAGVSPSLVAGVILAESGFDPKAQSEVGALGLMQLMPDTARWIAEAHQIEVEQTDN
RRLQEPELNIFLGAYYLAWLLQRFDNKSVEALAAYNAGQHEVDSWIEEQGGPLTVEEIPV
PETRFFVKKVLNNAEKYSKLYPQLICEQSSPVSRVEKNG

Enzyme Prediction     

No EC number prediction in MGYG000002669_00629.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	67	198	2.9e-30	0.8296296296296296

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd16896	LT_Slt70-like	5.53e-62	52	196	1	146	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	2.36e-58	51	200	2	152	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
cd00254	LT-like	1.10e-40	70	195	1	111	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
COG0741	MltE	4.92e-34	46	203	130	289	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
pfam01464	SLT	1.31e-33	59	174	1	111	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARF99123.1	3.65e-46	51	201	32	180
BBU36008.1	1.66e-44	47	201	28	180
VEG93728.1	1.66e-44	47	201	28	180
BBU34602.1	3.33e-44	47	201	28	180
ACZ24461.1	6.67e-44	47	201	28	180

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MPQ_A	8.18e-21	48	201	411	559	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
5O1J_A	8.21e-21	48	201	415	563	Lytictransglycosylase in action [Neisseria meningitidis MC58]
6FPN_B	8.26e-21	48	201	421	569	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	8.29e-21	48	201	425	573	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	8.33e-21	48	201	431	579	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39434	1.38e-20	43	191	469	619	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P0AGC4	5.44e-19	43	191	469	619	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
P0AGC3	5.44e-19	43	191	469	619	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
O64046	3.04e-18	51	196	1418	1543	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1
O31976	3.04e-18	51	196	1418	1543	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
21	43