CAZyme Information: MGYG000002673_00297

Basic Information

• Species: UBA9475 sp900549885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA9475; UBA9475 sp900549885
• CAZyme ID: MGYG000002673_00297
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
811	MGYG000002673_2|CGC1	89666.09	5.3748

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002673	2826346	MAG	Canada	North America

• Gene Location: Start: 101913; End: 104348 Strand: -

Full Sequence      

MKHELFNDNWKFWEDKDAFALIWSVPDNAQTVRLPHDAMIGARAYADSPAGADGAYRDGA
SYVYYKELYAPERWRDQTLKLYFEGVSRSAMVYVNNQRAALVPYAYTAFYVDLDDYLKYG
QVNQIRVCVRTGDMPNSRWYAGGGIYRDVYLLEGGLAHIPPQGLRVTTASLDEDMAVIQV
DTQLVNRIHHPVAVEVQTKLYGPDGALAAEETTPLTLMGGSKEPLKQTLAVAGPHPWSDL
SPDLYTCRVTLTAVGQELDSAETAFGIRTISVDARRGLRINGRSVKLRGACIHHDGGLLG
AATFEEAEFRRMRLLKEAGFNAVRSAHNPMAPVALRACDVLGIYVMDEAFDMWTRSKKDY
DYSQVFEKWWRDDLAAMVKKDYNHPSVILYSIGNEIPEIATAHGAALGREMRRVVERLDP
TRPSLACINGVFATGDRLDEIIADVLQSSDRPGGGGAGNVNDFMAATSTHADEIANHPLI
SRNLELAGAGMELMGYNYMTARHEPDGARYPNRVIVGSETYTPEIARNWDIVERCSHVIG
DFTWTGWDYLGEAGVGVPAYRPGEGSFGAQYPCQLAYVGDIDITGFRRPASYFREIVFGL
RKDPYITVQDPTHYGQQPMQTPWVISDNYASWTHPGFEGRPVVVEVYSPGDEVELLLNGA
SLGRRAAGKEAGFRALFETVYRPGRLEAIAYEKGRELGRSELTTAQNPLVLRVQPEETDG
RELIYVNITLEDGHGVCMNTDTGISALVEGDGGLAGFGSADPKPLTGYTDGVSRMFHGRA
QAIVRKTARNGMVALTVGAEGYKPVRMEIRF

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	625	9.7e-98	0.6117021276595744

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.75e-58	6	423	15	428	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.31e-40	10	446	19	467	beta-D-glucuronidase; Provisional
PRK09525	lacZ	2.21e-35	112	423	173	484	beta-galactosidase.
PRK10340	ebgA	6.46e-32	47	422	107	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam16355	DUF4982	7.47e-21	639	699	1	61	Domain of unknown function (DUF4982). This family is found in the C-terminal of uncharacterized proteins and beta-galactosidases around 680 residues in length from various Bacteroides species. The function of this protein is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF42782.1	3.25e-299	1	800	1	807
SNU91084.1	2.36e-293	1	796	1	795
QBE95487.1	4.18e-284	1	809	1	816
VTS80520.1	1.28e-282	1	795	1	793
QQA36833.1	2.52e-277	1	796	1	794

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6B6L_A	7.51e-132	1	796	5	762	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	8.14e-132	1	796	5	762	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
7CWD_A	4.38e-104	1	801	1	791	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	8.06e-102	6	801	12	797	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	8.33e-96	6	809	30	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	5.71e-99	6	809	47	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.11e-72	6	712	51	715	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.61e-32	4	473	54	507	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.16e-28	64	795	60	721	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A1SWB8	1.00e-25	45	423	106	482	Beta-galactosidase OS=Psychromonas ingrahamii (strain 37) OX=357804 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002673_00297.