CAZyme Information: MGYG000002716_00075

Basic Information

• Species: F23-B02 sp900556535
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; F23-B02; F23-B02 sp900556535
• CAZyme ID: MGYG000002716_00075
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
792		86321.93	7.0546

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002716	1923088	MAG	Canada	North America

• Gene Location: Start: 103064; End: 105442 Strand: +

Full Sequence      

MKYARLISKMTLEEKASLMSGADFWHTKAVERLGIAAMMLTDGPHGLRKQIGKADHLGLN
AAYPATCFPTASCAANSWDEELIEQMGRRLGAEARAQRVSVVLGPGVNIKRSPLCGRNFE
YYSEDPLLAGKCAAALIRGIQSQGVAACVKHFAANSQETLRMTSDSVVDERALREIYLPA
FEIAVKEGGVKCLMTSYNKLNGQYTNENTHLLRDVLVRDWGYDGLIVTDWGGDNDRVRGL
IAGNALEMPGTGGETDRQIVRAVRAGELDEALLNERVDSVLRLVFDGESAFENASEVSFD
EHHEFAARLAAESAVLLKNEGGLLPLKKGARVAVIGDFAAVPRYQGAGSSHIEPTRLPSG
LDELRAEGINVIGYERGFKRGGGRSARLASRAAALARKADVVLLWLGLDEGSEAEGVDRQ
HMRLPENQLELLRSVRKANENIAVILSCGSPVETDWDADAKALLHGYLGGQAGAAAIARL
VSGRVSPSGKLAETMPHRLSDTPCRDYYPGPESTSEYRESIFVGYRYYDSAKKDVKYPFG
FGLSYSEFEYSELEIRGGEVSFNIKNVGGVSAAEVAQVYVSAPKTGVFCPEKELRGFVRV
ELSPGQEKRVSVALPERAFCYWNVSLDRWVCAGGEYGVLVGASSRDIRLAGSIEKQGDAA
PDPYADEIFAPYRSADVQCVSDAAFSALLGRELPPHLWDRNAPLEFNSALVQGKYRPGIG
RLLYRLLRLVYGFFRVTGNREGANNTVFAMNLPYRGLARFTGAVSEAQVRALLSAANREK
GGKRALIRSFLP

Enzyme Prediction     

EC	3.2.1.21	3.2.1.37	3.2.1.23	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	28	257	9e-67	0.9212962962962963

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	3.37e-69	23	369	48	391	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PRK15098	PRK15098	1.27e-65	6	648	41	758	beta-glucosidase BglX.
PLN03080	PLN03080	3.46e-50	6	612	56	740	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	4.52e-45	314	545	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	3.68e-35	32	283	63	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZK48658.1	8.37e-297	1	764	1	768
AZK49185.1	3.04e-293	1	777	1	781
AST98591.1	2.82e-288	1	777	1	782
QNM45585.1	2.82e-288	1	777	1	782
QHZ46306.1	2.53e-287	1	764	1	767

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	8.20e-220	5	653	7	666	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	4.91e-186	7	658	9	666	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	1.26e-129	2	652	3	710	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	1.91e-128	2	652	3	710	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	1.61e-110	5	647	8	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	4.49e-219	5	653	7	666	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	9.04e-179	7	666	9	683	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	5.07e-178	7	666	9	683	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
P27034	1.90e-131	6	663	5	818	Beta-glucosidase OS=Rhizobium radiobacter OX=358 GN=cbg-1 PE=3 SV=1
A7LXS8	1.11e-122	7	648	50	738	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000040	0.000008	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002716_00075.