CAZyme Information: MGYG000002717_01848

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000002717_01848
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1040		114936.65	9.1561

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002717	5602088	MAG	Canada	North America

• Gene Location: Start: 1048; End: 4170 Strand: -

Full Sequence      

MKKLLLSFLMICSCCAGLNAQAHSLPAQQSPIVPVEPILIRQFSGDEQCRQWVDSVMDRM
NLKERIGQLFIYTIAPQQNKANRDLLRKVVDDYKVGGLLFSGGLIENQVALTNEAQKMAD
VPLMITFDGEWGLSMRLRGTPVFPRNMVLGCIQDDKLLYEYGREMARQCRELGVQVNFAP
VADVNINPKNPVINTRSFGESPANVADKVIAYSRGLEEGGVLSVSKHFPGHGDTDVDSHH
SLPVLSFSRARLDSVELYPFRKAIQAGLSGMMVGHLEVPVLEPKRGVPSSLSRKVVHDLL
TQEMKFQGLVFTDALAMKGVSTTDQTVCLKALKAGHDLLLVPRRIKEEVDAVLNAVKNGE
LTEAEIEAKCRKVLTYKYALGLSKKPFVRLSGLGNRINTAHTRDLIRRLNQAAITVLQNK
GNMLPLDADTREVAVLNVGDAKEIQPFLKELSGYIKASTAVFQLKKELPEVARKQLRDSL
LQYKRILVCVTDHRLIPYQAFFNEFAPDVPVAYLLFIPGKQVLQIRRGVSAADAVILAHS
SMDDVQEQVARILYADATADGHLSASIGNLFATGDGLAITPKTPRHFVPDEYGVNSRLLA
RIDDIAKEGIKEGAYPGCQIVVLKDGKEMYNKAFGTHTWGGIVGISANKLPAAVSVPGAG
LPVSPTDVYDLASLTKTTATLLAVMKLYDKGRLSLTDRASDYLPWLQDTDKKDITIRQLL
LHESGLPSTLLFYQEAIDEDSYTGTLFKARPDATHSVQIGTRTWANPKFRFKKGLTSKVR
TAEHTLQVSDSLWLNRSFKEEYRQKIVKAPLRDRRYRYSCVGFILLQQVVEARTGMSMDA
FLEQEFYAPMGLKHTGYLPLQGAAAVGIASGTYTPVPKAEIIPSSIDPFLRKTVLQGFVH
DESAAFLGGVSGNAGLFSNATEVARIYQMLLNGGELDGKRYLSPETCRVFTTTVSRISRR
GLGFDKPDRQNPAKSPCAAAAPATVYGHTGFTGTCAWVDPTNGLVYVFLCNRIYPDVWNT
KLMKLDIRTRIQEVVYQALR

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	115	341	1.8e-55	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	1.80e-78	60	436	1	360	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	2.40e-78	62	375	2	315	Glycosyl hydrolase family 3 N terminal domain.
PRK03642	PRK03642	1.36e-53	589	1040	30	432	putative periplasmic esterase; Provisional
pfam00144	Beta-lactamase	2.44e-44	602	1017	1	313	Beta-lactamase. This family appears to be distantly related to pfam00905 and PF00768 D-alanyl-D-alanine carboxypeptidase.
COG1680	AmpC	4.06e-42	596	1016	36	367	CubicO group peptidase, beta-lactamase class C family [Defense mechanisms].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO71487.1	0.0	1	1040	1	1049
ALJ59284.1	0.0	1	1040	1	1046
QUT89658.1	0.0	1	1040	1	1046
QUT62072.1	0.0	1	1039	1	1009
QQA30538.1	0.0	1	1039	1	1009

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K5J_A	2.48e-62	60	458	11	420	Structureof a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
3BMX_A	1.34e-58	54	425	36	446	Beta-N-hexosaminidase(YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	3.87e-58	54	425	10	420	ChainA, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	6.82e-58	54	425	40	450	Crystalstructure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]
3SQL_A	1.01e-52	30	402	7	371	CrystalStructure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQL_B Crystal Structure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQM_A Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_B Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_C Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_D Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40406	7.35e-58	54	425	36	446	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
P48823	7.16e-45	86	458	55	456	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
P77619	3.98e-40	589	1017	30	400	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli (strain K12) OX=83333 GN=yfeW PE=1 SV=2
Q8XBJ0	1.09e-38	589	1017	30	400	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli O157:H7 OX=83334 GN=yfeW PE=3 SV=1
O05213	4.56e-37	571	1017	24	409	N-acetylmuramyl-L-alanine amidase OS=Bacillus subtilis (strain 168) OX=224308 GN=amiE PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000702	0.719000	0.279341	0.000375	0.000305	0.000252

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002717_01848.