CAZyme Information: MGYG000002717_03104

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000002717_03104
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
939		104309.32	5.1477

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002717	5602088	MAG	Canada	North America

• Gene Location: Start: 327; End: 3146 Strand: +

Full Sequence      

MKRIITTCLLLSGTCLLSAQKIPSVYKPAKSEMYRKGWIDFNKNGVKDVYEDPNATLDAR
IDDLLSQMTLDEKTCQMVTLYGYKRVLKDDLPTPEWKQMLWKDGIGAIDEHLNGFQQWGL
PPSDNPNVWPASRHAWALNEVQRFFIEETRLGIPVDFTNEGIRGVESYRATNFPTQLGLG
HTWNRELIHRVGLITGREARMLGYTNVYAPILDVGRDQRWGRYEEVYGESPYLVAELGIE
MVRGMQHNHQVAATGKHFVAYSNNKGAREGMARVDPQMSPREVEMIHVYPFKRVIKEAGM
LGVMSSYNDYDGIPIQGSYYWLTARLRGEMGFRGYVVSDSDAVEYLYTKHSTAKDMKEAV
RQSVEAGLNVRCTFRSPDSYVLPLRELVKEGGLSEDVINDRVRDILRVKFLIGLFDAPYQ
TDLAGADKEVEKAENEAVALQASRESLVLLKNENNILPLDINNVKKIAVCGPNANEEGYA
LTHYGPLAVEVTTVLEGIRQKTEGKTEVLYTKGCDLVDANWPESELIEYPMTDEEQAEIN
KAVENARQADVAVVVLGGGQRTCGENKSRSSLDLPGRQLKLLQAVQATGKPVVLVLINGR
PLSINWADKFVPAILETWYPGSKGGTAVADVLFGDYNPGGKLTVTFPKSVGQIPFNFPCK
PSSQIDGGKNPGPDGNMSRVNGALYPFGYGLSYTTFEYSDIEISPKVITANQKATVRCKV
TNTGKRAGDEVVQLYIRDMLSSITTYEKNLAGFERIHLQPGETKEVTFTLDRKHLELLDK
HMEWVVEPGDFSIMVGASSEDIRLNGMLTVEDPNAPMQTQVQTDSPVTASTNPESIMNVL
DKKMNTVWEGNKGDYITFALENGSKVDGVSIAFSRSNGLPAEFEIQLSGGGGQFLTVYSG
TVSEYGKLISYTFKGTTASDLRIVLNDDRVGVAEVKIND

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	147	372	2.6e-64	0.9814814814814815

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	0.0	48	803	22	758	beta-glucosidase BglX.
PLN03080	PLN03080	4.49e-94	54	796	44	769	Probable beta-xylosidase; Provisional
COG1472	BglX	8.82e-77	134	500	41	393	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam01915	Glyco_hydro_3_C	5.19e-71	447	693	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	1.14e-61	69	408	1	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALJ61698.1	0.0	1	939	1	942
QUT92742.1	0.0	1	939	1	942
QDO69526.1	0.0	1	935	1	938
QUT62569.1	0.0	1	936	1	941
QQA31118.1	0.0	1	936	1	941

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XXL_A	7.79e-129	138	803	69	745	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	4.20e-128	138	803	69	745	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5YOT_A	3.38e-122	50	811	7	757	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
5TF0_A	2.46e-121	138	803	68	744	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
7EAP_A	2.54e-121	50	811	7	757	ChainA, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KMH0	6.85e-145	150	800	62	711	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
P33363	2.90e-131	58	803	35	758	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	3.09e-130	58	803	35	758	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
T2KMH9	2.19e-126	1	803	1	748	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
A7LXU3	1.11e-98	54	814	32	780	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000665	0.997887	0.000816	0.000214	0.000202	0.000174

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002717_03104.