CAZyme Information: MGYG000002717_03241

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000002717_03241
• CAZy Family: CBM38
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
879		99024.12	6.2802

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002717	5602088	MAG	Canada	North America

• Gene Location: Start: 34; End: 2673 Strand: +

Full Sequence      

MGRGDKTYGLPRFEEATFQARFPFATVDLRDKDFPLQAKIIGWSPFIPTDPDNSSLPVGI
LEYQFTNLSDKVLETVFSYNTKNFIDGQGTIQGVKNGFVLESNQNDSGLAIYVDNDEAVV
DYCWFRGGWFDSQTVVWDNIRCGRIANNQPVKGVAPGASVYVPVALQPGETKTVRVNFCW
YFPNSNLSIGFAKKAGKAYIGSPSKGTAPNQQSVSGFAGKQLLNSFDKGGDGLVGIIQSP
EFNIGKRYLKFLVGGGNQPDRTSVNLVIDGKIVQTAAGNQSEILNEVVWDLKLFQGKKAI
LKVIDLDVYPWGHILADQFVLTNDENENLYDLSSTATVLADFEDNSWKDWQVMDSSEEEK
QFLVNEEDTETTYRPWYSKRFKNLNEVIGYWDANHATLKKNSKLFSDAFYDSTLPDEVLE
AIAANLTILKSPTVLRQWDGRFWAWEGCQDSWGSCHGSCTHVWNYAQALPHLFPSLERTL
RETEFRVAQNAKGHQNFRVNMPISPPPHDFHAAADGQLGGIMKVYREWRISGNTQWMKDL
FPAVKKSLDYCIRTWDPLQKGYLEEPHHNTYDIEFWGPDGMCTSFYLGALTAFIEMSKAL
KQSTKKYDILLAKGKKYMENALFDGEYFIQKIQWEGLQAPNPVDVLSFGGGYSDEAMKLL
KKEGPKYQYGSGCLSDGILGMWMASVCGLNEVLNNEKVKSHLVSVHKYNLKDNLVDHFNP
QRPAYACGKEGGLLLCTWPKGGMLSLPFVYSNEVWTGIEYQVASHLMMKGEVAKGLDIVR
ECRKRYDGRVRNPFDEIECGHWYARAMASYGMLQGLTGVRYDAVNKTMYIDSKIGDFRSF
ISTETGFGTIEWKHGKPSLNVVYGIIDVKHYNVSGKTMN

Enzyme Prediction     

No EC number prediction in MGYG000002717_03241.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	440	812	2e-62	0.9944903581267218
CBM38	195	305	7.7e-28	0.8604651162790697

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	1.49e-64	440	812	1	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
pfam12215	Glyco_hydr_116N	2.65e-27	14	190	77	277	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
COG4354	COG4354	2.81e-23	376	820	279	721	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT37384.1	0.0	1	879	114	992
BBK86457.1	0.0	1	879	114	992
QUT61530.1	0.0	1	879	114	992
QQA30036.1	0.0	1	879	92	970
AGU13697.1	0.0	1	879	114	992

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	2.34e-08	521	803	520	754	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5BVU_A	2.36e-08	521	803	524	758	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	2.36e-08	521	803	523	757	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
7DKS_A	2.36e-08	521	803	524	758	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKT_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKU_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKV_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]
7DKW_A	2.36e-08	521	803	524	758	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKW_B Chain B, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKX_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKY_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
K0E4E1	5.87e-20	202	328	340	465	Putative glycosyl hydrolase ecdE OS=Aspergillus rugulosus OX=41736 GN=ecdE PE=3 SV=1
Q9HCG7	3.00e-09	508	808	612	876	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q5M868	5.14e-09	508	808	603	867	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q69ZF3	7.88e-08	508	808	603	867	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002717_03241.