CAZyme Information: MGYG000002721_01772

Basic Information

• Species: CAG-873 sp009775195
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873; CAG-873 sp009775195
• CAZyme ID: MGYG000002721_01772
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
669	MGYG000002721_17|CGC2	74349.85	6.9261

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002721	3174810	MAG	Canada	North America

• Gene Location: Start: 64381; End: 66390 Strand: -

Full Sequence      

MKRILFSILSAALLSAGAVAEVSAPGYPITPVPFTSVHVSGPFWGARIEASRNVTIPLAF
AKCEENERYANFVRAAHPSEEYKVTGFPFDDTDVYKTIEGAAYRLHNYPDKKLEAYIDSV
IDIMAAAQEPDGYLYTARTQNPAHPHEWAGKERWTSVENLSHEFYNLGHMIEGAVAYYQA
TGKRKFLDIACRYADCVDRNIGEGEGKLRLVPGHQIAEMALVRLYNVTGEKRYFDLAKFF
LDARGTTARRQQYSQSHIPVVQQSEAVGHAVRATYMYAGMADVAAISGDTSYIHAVDRIW
DNIVGKKLYITGGIGARHRGEAFGDNYELPNASAYNETCAAIGNVYTNYRLFLLHGDAKY
YDVLERTLYNGLISGVSIDGGSFFYPNPLASTGNYSRKPWFGCACCPSNISRFIPSLPGY
IYAVKDKSLFVNLFIPNTARLDVNGQRVTVSQSTSYPWDGNIKLTIDKGRADMDLKIRIP
GWVRNQVVPGDLYSYVDNEKPSYSVRVNGEEVSGQLDNGYLVIPRKWRKGDVVDIVFDMP
VRTVKANDKVVDDRGRIAVERGPLVYCAEWPDNDVNIHTVLLRRNPSYTLGGHDSDLGGI
TRISTDAQALSYDNDGRLVVSPTKLTLIPYYAWAHRGEGNMAVWLPVDLSAATASAPAAT
KNEDNGFFR

Enzyme Prediction     

No EC number prediction in MGYG000002721_01772.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	37	567	5e-208	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	31	645	11	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	0.0	36	567	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT26933.1	0.0	26	669	24	667
QPH58081.1	0.0	26	654	28	664
QBJ17728.1	0.0	26	654	28	664
QMI79221.1	0.0	26	654	28	664
BCA48229.1	0.0	26	669	24	667

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	0.0	26	669	4	647	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	1.64e-145	35	645	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.99e-140	35	645	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.01e-122	86	644	70	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.38e-122	86	644	70	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	5.56e-122	86	644	70	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000370	0.998930	0.000197	0.000185	0.000156	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002721_01772.