CAZyme Information: MGYG000002774_00480

Basic Information

• Species: Schleiferilactobacillus harbinensis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Schleiferilactobacillus; Schleiferilactobacillus harbinensis
• CAZyme ID: MGYG000002774_00480
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
744	MGYG000002774_2|CGC1	81307.17	5.6059

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002774	3078759	MAG	United States	North America

• Gene Location: Start: 14964; End: 17198 Strand: +

Full Sequence      

MEQAKLHDLLQSLTLQEKIGQLIQLSGEFFDATDISLGPQRKLGITQEAVDLAGSVLNVA
GAKEVKRIQDIHLRQGGHPIPLLFMADVIYGYKTVYPIPLGIGATWDPALVERAYTNTAD
EARASGLQVSFAPMVDLVRDPRWGRVLESTGEDPYLNSRFAAAMVKGFQKNLAAGSGLVS
CVKHFAAYGAAEAGREYNPADLSTPTLFQDYLPSYKAAIDAGVKMVMTSLTTLNGVPATA
DKWLLQDILRKRWGFDGVIISDYASVYELIKHGFAQDSTDAAAKALTAGVDIDMKSPCYA
NGLQPLVENGHLSEAKIDDAVWRVLTLKNELGLFEHPYGGATEEREHAQTLTPEKRSLAR
DLSRKALVLLKNNENLLPLNPHGNQRVALIGPYADEQELLGLWAVHGERKDTVTIKAGFS
ECLPAQQLQIAKGTDIIRDRAMLEKMGVSAEQIGHIISGSNTEQSNHDQALAIAKNADII
VFAAGEHTLQSGEAGSRTRLRLPDNQRQLLAELRKLNKPIVLIVISGRPLVLTDVQDQAD
AILQAWFPGTEGGHAIADVIFGRYNPTGRLSMTFPYDEGQLPLYYNHLSTGRPENHSQHS
GRFVSRYIDNPNKPLYPFGYGLSYGSVQYGELKLDSDTLAAAGRDELTAAISVTNTGTVP
REETVQFYIHDQVASIVQPVKRLIGFQKVPIQPGETKTAALAITPDMLSFANRDGEFITE
PGMFDLFAGPNSQDVHQTSFSLTE

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	80	294	1.7e-66	0.9537037037037037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	0.0	3	742	35	764	beta-glucosidase BglX.
COG1472	BglX	3.91e-99	13	398	1	368	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	1.48e-85	14	327	1	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	1.18e-74	94	729	112	769	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.55e-60	367	624	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFR24638.1	0.0	1	744	1	744
QEU47574.1	0.0	1	744	1	744
QHM47982.1	0.0	1	742	1	740
ATO54089.1	6.85e-318	1	742	1	738
QFQ74402.1	4.26e-312	1	742	1	739

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZOA_A	2.27e-200	1	735	1	712	CrystalStructure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOA_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOB_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOB_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOD_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOD_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOE_A Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262],4ZOE_B Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262]
4ZO6_A	3.63e-199	1	735	1	712	CrystalStructure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO6_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO7_A Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO7_B Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO8_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO8_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO9_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZO9_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZOC_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262],4ZOC_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262]
5XXL_A	2.11e-194	9	742	17	751	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.19e-193	9	742	17	751	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5TF0_A	6.50e-188	9	742	16	750	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56078	4.36e-189	8	742	40	764	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	6.24e-183	8	742	40	764	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH0	2.16e-114	77	743	71	721	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
T2KMH9	1.70e-95	9	742	43	754	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
A7LXU3	4.80e-93	10	735	43	768	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000040	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002774_00480.