CAZyme Information: MGYG000002774_00654

Basic Information

• Species: Schleiferilactobacillus harbinensis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Schleiferilactobacillus; Schleiferilactobacillus harbinensis
• CAZyme ID: MGYG000002774_00654
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
598		67518.77	5.847

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002774	3078759	MAG	United States	North America

• Gene Location: Start: 196291; End: 198087 Strand: -

Full Sequence      

MLYPRATDSRAVVDLSGVWQFKLDDGHGFADHWYAKPLTAPETMAVPGSYNDQKADAAHR
DHYGWAFYQRTITVPPFFAGQRIVLRFASVTHTAKVYLDGTEIATHEGGFLPFEIDLTDQ
LTLGQSALLTVAVDNRVNFSTLPVGNEDGGSMFGMGDGGIPSVKIAQEHAKKQNQPNFDF
FNYAGIQRPVKLYTTPKAYIRDITLVPDIAGTDGLVKYTVATQGAGETTVEILDRTGQVV
AHGAGTTGTLTVPNAHLWAPGHPYLYTAVVTFSADQYRQTFGIRTVRVEGTKFLINDQPF
YFKGFGKHEDSPLHGRGFDAVFDVADLSLMHWIGANSFRTSHYPYAEEMYDLCDREGIVV
TDETPAVGLGRGTQADAYKTMHNGELHRQNLRDMIDRDKNHPCVVMWSLANEPETAAFPQ
SAYDYFRPLYELAHELDPENRPVTLVNAQSDYNKDMITRTMDVVCINRYYGWYNLGGDLD
AACEAWNIELDFWAKQHKPVMVTEYGADTVEGLHDVVPRMFDEEYQAEYFDRISAQFDKR
PFFIGEQIWNFADFNTIQGLVRVDGNRKGVFTRTRRPKMVAHTIRRRWHTIPDFGYKA

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	7	580	1.1e-111	0.6037234042553191

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	1	597	1	601	beta-D-glucuronidase; Provisional
COG3250	LacZ	2.39e-117	1	588	1	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	2.87e-81	286	588	1	297	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	9.03e-45	9	438	38	468	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.61e-27	7	412	47	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFR24827.1	0.0	1	598	1	598
QFR63248.1	0.0	1	598	1	598
QEU47766.1	0.0	1	598	1	553
CBL12719.1	4.77e-289	1	597	1	599
CUO31707.1	3.18e-287	1	597	1	600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U7I_A	1.46e-269	1	597	1	597	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]
6LD0_A	7.96e-241	1	597	3	669	ChainA, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1]
5Z1B_A	2.77e-239	1	597	25	691	Structureof Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_B Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_C Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_D Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679]
6BO6_A	1.28e-222	1	597	1	609	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]
6BJQ_A	2.83e-222	1	597	25	633	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	3.72e-181	1	597	1	596	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P06760	2.59e-148	1	593	27	627	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
P12265	3.65e-148	1	593	27	627	Beta-glucuronidase OS=Mus musculus OX=10090 GN=Gusb PE=1 SV=2
Q4FAT7	4.13e-148	1	593	28	631	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
O18835	2.25e-147	1	596	27	634	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000008	0.000043	0.000007	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002774_00654.