CAZyme Information: MGYG000002774_02147

Basic Information

• Species: Schleiferilactobacillus harbinensis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Schleiferilactobacillus; Schleiferilactobacillus harbinensis
• CAZyme ID: MGYG000002774_02147
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
785	MGYG000002774_10|CGC2	87669.94	6.1192

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002774	3078759	MAG	United States	North America

• Gene Location: Start: 42864; End: 45221 Strand: +

Full Sequence      

MEQIQLRTWRFSHTDAQSTWQKDFDDTDWERVSVPHDWSVHEPFDRHNASGTGYLNGGVG
WYREHFSLTALSRSDHDTVILKFAGAYRNSDVWVNGYHLGGRPSGYAEFSFDITDLLADA
PDDDLVIVIRLARHELADSRWFNGTGLNRPVYLEVHQALMIPDYGTTFTTPAVTADQATV
QIRHQVRNRLVNTHLVTVTDTLTRLDTGQTVSLTHTETIKSNEEKVVTSSTVLPQPALWS
VDHPHLYRLTTTLTSDSSDVQSTYTQIVGIRNAVFDPNTGFHLNGQALKIKGVCLHEDAG
VLGTAVPAPVWTRRLLALKKMGANAIRMSHNPHTPALYNLCDQLGFLVFDEAFDEWENPK
NKWWQGHNVYPPKFGGYAHDFPHWHAADLANMVQRDRNHPSIIAWSIGNEVDYPNDPYAN
PLFKEMTGNNDANKPAAEREYNPDRPDTRRLSVIAAELAKIVKANDDTRPVTLAAAFPEL
SARTGLLNALDVVGYNYKEHLYAQDHERFPTKPFIGSENGHDYAAWRAVTGHDYVAGQFL
WTGIDYLGEAQGWPIHGSGAGLLTLAGFPKTPYYLRQSWWAKTPVLKMVTVPQGTLHYPW
EPLYRRWDYVSGSAITVRFYTNCQHLQLTLGTTPLSAPVYDAKHGYYETTIAYKNEPLLV
RGTYQGAQVTDTLVPVGAPAQLHAAVWSDPQAPKTADDDVYQIEITLLDQAGHPTEAEQI
IHAAVAGGTLLGLENGDLADTTDYSADYRRTLAGHLVIFVRSTDPAHTTVTCQTNGLAPV
TMTLQ

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	569	4.6e-109	0.5851063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.73e-66	18	598	49	556	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.06e-34	8	413	47	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.69e-32	9	410	59	462	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	1.21e-29	281	551	8	250	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	1.95e-26	61	448	68	446	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFR63016.1	0.0	1	785	1	785
QFR24569.1	0.0	1	785	1	785
QEU47483.1	0.0	1	785	1	785
AZP04142.1	7.67e-310	1	777	1	790
AUJ86441.1	3.58e-303	1	785	1	798

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T9A_A	1.58e-136	9	766	36	824	Crystalstructure of BuGH2Cwt [Bacteroides uniformis],5T9A_B Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_C Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_D Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9G_A Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_B Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_C Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_D Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis]
6B6L_A	1.23e-106	2	762	7	752	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	2.59e-106	2	762	7	752	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4YPJ_A	3.46e-98	24	762	34	782	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	7.47e-98	9	762	10	776	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.86e-112	9	777	51	839	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	4.18e-77	9	762	55	795	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	9.33e-37	26	431	76	455	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
B4S2K9	1.05e-35	29	545	82	571	Beta-galactosidase OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) OX=1774373 GN=lacZ PE=3 SV=1
P77989	8.31e-33	9	407	12	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999996	0.000023	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002774_02147.