CAZyme Information: MGYG000002783_01361

Basic Information

• Species: Prevotellamassilia sp900541335
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotellamassilia; Prevotellamassilia sp900541335
• CAZyme ID: MGYG000002783_01361
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1296	MGYG000002783_34|CGC1	146354.67	6.3452

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002783	2416856	MAG	United States	North America

• Gene Location: Start: 14270; End: 18160 Strand: -

Full Sequence      

MKQFKKLLTVSCCLVTMTAQGQFTSVNELPQGQDSRMSYMLTTPRGSLFSDPTIEASCLT
SNCQTGRNEKIKLSATDARGQFLLFKAKNEPNGYYIYSVKAQSFLGKPGGDSGVKCPYVD
KPQVLYVWKNGQSGSQNNGYGVNYEQSEYPFSIGAVDGTQGAPYTTINVCCWDQNNPDYR
FCETSGYDAGNEYAISEAQKVNETLFQLAYQKVVEAEKQPETGTTDPLDPNNVLSTEERK
TQTQAALDIINRFTEGTMKVKLDLSLCRTQSGCDRYTYSATTDKLTIKASSAVAACRGFY
DYVKSKGAGFASWSGTRFQKPADMSCSEVSLTTQFRDHQYFNVVTYGYTVPFWDEARWDK
ELDWMALHGIDMPLMLVGAEQIYREVFYDMGLNKEEVDAWEVGPAHLPWFRMGNVAGNSF
DGPLGEEWNEKQRKLAHHLLDRMRALGMKPICPAFGGFVPQAFTKHHSRKTELTGWNWVP
TSHRNYRLDPGSAAFVEVGKRFIQKWEKEYGVGKYYLSDSFNEMIIPSSTATLTQYGDSI
FKSIREGSANPDAVWVTQGWTFVYQSGQWGSSKFNALTQNIPDHRFMVLYMSPEYGPNKC
WERYDGFNGKEWCYTMLPNMGGKTFWTGNFSAYANQYLTNLYRSTSKGRVTGYGLTPEGV
ENNEMLYELVCDAGWTPANGTIDLDAWMERYAQSRYGKHPQTVRDFLKVLRQTVYNKYID
HPRFGWQMGGNITGTGSASLADAYYKGVETLFAQTEELKQQQSPLLENELIEAAVLYACG
RIENLNTRILGLKKNPKEAQRLIAELDTLMLDMDAALARHPLYNLKHWEDQAQGMADTPE
TKLRNARNARRIVTVWYGDHQTNEPVQDYAARIWSGLIRDFYRPRLIQTLKHKVGFLPSF
NSIEFENKFVAAAPTLSQPKEIEGNLIDFLAQLVDRAKNCAHVQVEALQAIETSNDSINH
WYVVRSGYEAQLDKVLTASGDYQKLEALTDVRDGYQAWRFIRTGENAYRLENRWGENLNV
TDSELRTLYAHASSDLHVEWTSDGTHRFAILPQAAPKDKPALHCNGNLMLYGYKGNDGYY
AGSLWTIEPMPTALVPEAKSEDYTRYIRRLTGFTQQELFGKPGQPVSADALNLAVNRLYD
EAQNIDHKTYDTFLAQWAELWKKTFVLPQGEEANRLLQLIVSAHQLVQPADAAISTDAAS
LRKAILRAEKAVAGALTEQTTLEAEYNYLNQAILQYYQAKGGTVIQPGLPQGIQQTQAQA
MSQVIYDLQGRAIDAAELDRQSTPQVYICNGKKMIK

Enzyme Prediction     

No EC number prediction in MGYG000002783_01361.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	282	909	7.7e-157	0.9487179487179487

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	3.04e-127	340	677	3	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	7.99e-30	687	910	1	239	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO23473.1	3.68e-165	232	885	16	663
QWO92760.1	5.80e-157	234	885	25	673
QWP70100.1	5.80e-157	234	885	25	673
QWO85452.1	5.80e-157	234	885	25	673
QWO95290.1	5.80e-157	234	885	25	673

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4XWH_A	2.08e-72	284	933	51	707	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]
2VC9_A	2.61e-55	248	885	176	816	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	2.80e-55	248	885	184	824	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	1.77e-54	248	885	199	839	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	4.22e-73	284	885	94	743	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	1.75e-71	284	933	74	730	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000373	0.998723	0.000393	0.000177	0.000157	0.000150

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002783_01361.