CAZyme Information: MGYG000002786_01384

Basic Information

• Species: UMGS268 sp900540175
• Lineage: Bacteria; Firmicutes; Bacilli; Acholeplasmatales; Anaeroplasmataceae; UMGS268; UMGS268 sp900540175
• CAZyme ID: MGYG000002786_01384
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
560	MGYG000002786_19|CGC1	64458.89	8.1843

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002786	1606694	MAG	United States	North America

• Gene Location: Start: 11512; End: 13194 Strand: -

Full Sequence      

MFIFLILILFVFQPFTSLADVQKNPEIVITDEQGNELMHYMNYHRITPVDLERFDEDNLS
YLLHIEDRSFMTHSGFNLQRIFKTLTSNLFHNQRHGASTITQQYIKNLYLNNSKSISRKI
KELYYAIKLEQVATKEQILEGYLNCIYLGNDIYGFANASRYYFNTSYDRLTAQQMIALIA
LLNAPSYYSSHITAWEKRKNELAKILHDDHLLDTTVYQQVVQPLDFDHCAKLYPDALLYY
CDAVLNEFKQIAVKAKFNQKIIIQTRYHSAINDQKFTTNANIASITIDQDGFILSCIGDI
DYARSTFNIALNGKRDIGSTIKPLLYYEALKCGYSPSTTHYSAPFSFQYHDETISVSNYG
SIYPYQKINMKNALATSDNIYAIKTHLDLGMKTLVYHLRKYNIAAQALPSLALGSIGMSL
YQLIRIYSQFFTEGLYLEPKFIETVSLDDQAVFKAHPQKKQLLKSDYVKQIKELMNGMFD
SKIKHATGLGIASLLKEKCYGKSGLTDYDSYMIGFSNDILIAVWSGHLDNRELKDSATKK
LPKQIFLQQMNAYIKNKNDV

Enzyme Prediction     

No EC number prediction in MGYG000002786_01384.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	39	198	3.7e-51	0.8983050847457628

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	4.64e-103	3	556	32	599	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.45e-95	44	556	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.16e-60	62	557	83	722	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.40e-53	33	207	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
PRK14850	PRK14850	1.90e-39	62	534	173	674	penicillin-binding protein 1b; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QVK17409.1	6.46e-90	47	556	72	605
QNM12898.1	2.54e-88	17	560	32	588
QSI24238.1	3.48e-88	5	560	18	588
QJA01179.1	7.31e-87	5	560	18	588
QIX07808.1	7.31e-87	5	560	18	588

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	8.33e-27	17	557	11	726	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	3.25e-26	62	534	172	675	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	3.36e-26	62	534	193	696	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3FWL_A	4.21e-24	62	534	176	679	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli]
3UDF_A	1.28e-23	48	458	41	564	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	3.46e-68	65	555	93	609	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
Q9KUC0	2.48e-36	52	534	186	696	Penicillin-binding protein 1B OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcB PE=3 SV=1
P40750	1.36e-34	17	526	46	596	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
A0A0H2ZMF9	2.43e-34	47	526	112	622	Penicillin-binding protein 2a OS=Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) OX=373153 GN=pbp2a PE=1 SV=1
Q8DNB6	2.43e-34	47	526	112	622	Penicillin-binding protein 2a OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbp2a PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000316	0.999014	0.000180	0.000163	0.000153	0.000149

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002786_01384.