CAZyme Information: MGYG000002817_03908

Basic Information

• Species: Paenibacillus_B sp900539405
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_B; Paenibacillus_B sp900539405
• CAZyme ID: MGYG000002817_03908
• CAZy Family: GT2
• CAZyme Description: Dimodular nonribosomal peptide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2402	MGYG000002817_24|CGC1	262624.82	5.5517

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002817	6425839	MAG	United States	North America

• Gene Location: Start: 34029; End: 41237 Strand: -

Full Sequence      

MNDRDQDRWLLSGAQSGIWFAQQLDPDNPIFNAGEYIDIRGPVDPERFETAVRQAVMEAE
ALHARFGEDRDGPWQTIVPSSDWTMHRVDVSGEKDPHAAAEAWMKRDLAQPVDLGRGPLF
TEALLKLAPDRFYWYQRIHHIVIDGYGFSLLTRRTAQIYTALAQEMPIGEGAFGSWRSVL
EEDAAYRASDRLERDRQFWLARFADHPEAASLGDRARRTSRSFLRQSAELSPSVMDGWQA
AANGMGASWPDAVMAAAAVYMHRLAGTDDIILGVPMMCRLGSAALRVPGMVMNLLPLRAA
VRPDMSVNELVRQISEKMREIRRHQNYRHQDLRRDLKLVGDNRRLFGPLINIMPFIEELN
FAGCRGKVHNLAAGPVDDLTIHVYAGSGGSGPRIDFDANPAVYDAGELSAHRRRFLHALD
KLAASEPGQPIGRLELLLPEERLQVLERWNDTAHALAQWSVPALFEEQAASTPDAVAAVF
EDTALRYADLNARANRLAHWLIAHGAGPDRIVALALPRSLEMVVAILAVHKAGAAYLPLD
PDYPSARIAYMLNDAAPMCLITVRKAASRLPGSGSIPKLLLDEPAAMERLEQQSEGNPTD
ADRLCPLSPLHPSYVIYTSGSTGRPKGVLLTFGGLANLLLCMRERFAPDGRDRLLSVTTI
SFDISVMEVLLPLTTGASIDIAPKETILDPQALARKIKDTGATMMQATPTLWQALAACHS
GSFGGLTAITGGEALPIGLKLALQELDCEVNNQYGPTETTIYSTAALLGPERTGKPSIGG
PIWNTRLYVLDSSLQPVPPGAAGELYIAGAGLARGYLGRPDLTAERFVADPFGPPGTRMY
RTGDLARWLADGTIDYLGRADHQIKLRGFRIETGEIEDLLARHAGVAQAAAIVREDRPGE
RRLVAYVVPASSSGIDPVELRSYVASQLPDYMVPAAIVTLDAMPLTPNKKLDRKALPVPD
AGCTAAGRGPRTPQEEMLCALFAEALGVPRVGIDDDFFALGGHSLLAGRVILRIRESLGA
ELSIGSLFEEPTVAGLVRRLDDSREARPAIQPDRRTEDIPLSFAQRRLWFLYRLEGPAPT
YNIPLAARLSGELNIRALEAALGDVAARHETLRTVFPDEASGASRQLILDAGEARPRLIW
TRTSEAELLERLQEAVRYRFRLSDEPSLRAELFELGPNEYVLLLLLHHIAGDGWSLSPLT
RDLSAAYAARCRGEHPAWPPLPVQYADYAIWQERLLGSGGDPGSLMTRQLEFWTRTLAGL
PEQLELPADYPRPAAASHQGGTVHFHIHKELHGRLLDLARAGKASLFMVLQAALAILLTR
LGAGTDIPIGSPVTGRNDDALDGLVGLFINTLVLRTDTSGDPGFRELLARVRQVNLDAYA
HQDVPFERLVEAVNPVRSRSRHPLFQIMLVLQNTPAPELDLPGVDARLQLESAGTAKFDL
TVELQERRDAKGGPDGLDGIVEYSADLFKRGTAEALAARYVRVLESAAAAPDKPMGQLDI
LTPEERRRILTEWSASEAAAPPPAAIPELFEAQAGRSPGAAAVVHDGRALSYEELNRQAN
RLAHLLMAEGVGPEQLVALALPRSLEMVVGILAVLKAGAAYLPLDPDYPAERIAYMLEDG
APVCMVTIAEVSSGLPDTGGLPRIVLDETAVRERLRQQSSANPSDRERRRPLSPLSLAYI
IYTSGSTGKPKGVVIPHQNVVRLFASTMHWFRFGAEDVWTLFHSYAFDFSVWEIWGALLH
GGRLVVVPHAVSRSPREFLRLLAQEGVTVLNQTPSAFYQLMQADREQPDLGQALGLRYVI
FGGEALEPARLEDWYRRHPDHAPRLINMYGITETTVHVSYKELDRDSAASGANSFIGGPI
PDLRVYVLDKALQPAPPGTAGEMYVAGAGLARGYWGRPGLTAERFVADPFGPPGSRMYRT
GDVAKWLADGTLDYLGRADHQVKIRGFRIEPGEIQAVLARHPEVAQAAVVVREDRPGDKR
LAAYVVFAAGATLEPSELRRYAASILPDYMVPSAIIAIDSLPLTPNGKLDRQALPAPELS
LDLEGRNPRTPQEEVLCDLFAEVLGVRRIGIDDSFFELGGHSLLAVRLITRIREALGREA
GIGSLFEAPTVAGLAERLDMDGGASALQVLLPLRTHGGQLPLFCVHPAGGLSWCYAGLMK
HLGMDYPIYGLQARGIAQAEELPKTLEEMTADYIEAVRSIQPHGPYRLLGWSLGGNVAHA
MAVQLQEAGEEVSFLAMLDAYPSHYLPIRGEPDEEEALTALLALGGYDKDSIGGVPLTVA
NAIEILRSDSSALATLDEAAILNLKKTYENSVRLLGAYTPRRFEGDLLFYRSTIIPDWFD
PIEPEMWIPYIGGRIERHDIACRHKDLCQPGPLAEIGATLLAKLKSANGAAMRQDGRELI
HE

Enzyme Prediction     

No EC number prediction in MGYG000002817_03908.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF038078	NRPS_MxcG	0.0	6	1046	2	1049	myxochelin non-ribosomal peptide synthetase MxcG.
cd17652	A_NRPS_CmdD_like	0.0	1538	2035	1	436	similar to adenylation domain of chondramide synthase cmdD. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
cd17643	A_NRPS_Cytc1-like	0.0	1538	2034	1	450	similar to adenylation domain of cytotrienin synthetase CytC1. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK12316	PRK12316	0.0	7	1320	48	1346	peptide synthase; Provisional
PRK12316	PRK12316	0.0	10	1338	2604	3909	peptide synthase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	0.0	6	2261	558	2805
ACX49739.1	0.0	11	1775	12	1816
BAY90071.1	2.79e-244	971	2249	2131	3414
BAZ00088.1	3.48e-243	971	2249	2140	3423
BAZ75991.1	3.48e-243	971	2249	2140	3423

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U89_A	0.0	438	1510	3	1071	Crystalstructure of a cross-module fragment from the dimodular NRPS DhbF [Geobacillus sp. Y4.1MC1]
6MFZ_A	1.24e-267	464	2115	210	1794	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	8.02e-252	464	2041	210	1719	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
6P1J_A	7.38e-216	1060	2034	7	964	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae],6P1J_B The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae]
6OYF_A	2.37e-181	1058	1946	2	873	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module [Eleftheria terrae],6OZV_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with AMP [Eleftheria terrae],6P4U_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with Mg and AMP [Eleftheria terrae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q70LM5	0.0	11	2142	5189	7289	Linear gramicidin synthase subunit C OS=Brevibacillus parabrevis OX=54914 GN=lgrC PE=3 SV=1
P39846	0.0	6	2121	8	2079	Plipastatin synthase subunit B OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsB PE=1 SV=1
P39845	0.0	9	2146	6	2112	Plipastatin synthase subunit A OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsA PE=1 SV=2
P39847	0.0	6	2115	8	2070	Plipastatin synthase subunit C OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsC PE=1 SV=2
P27206	0.0	11	2116	8	2079	Surfactin synthase subunit 1 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAA PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002817_03908.