CAZyme Information: MGYG000002826_00766

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus
• CAZyme ID: MGYG000002826_00766
• CAZy Family: GH66
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1290		140319.39	4.4738

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002826	1064248	MAG	Singapore	Asia

• Gene Location: Start: 585; End: 4457 Strand: -

Full Sequence      

MNQQKNSVSEKGHGYFRKRNKALVSMITLFGALMVSSVAMADEVNTNPTSEIPATAQPVA
TNTSTPTTTSEEGATTEATSSPSESTPTVTATQPVSATTATTYNTPKITNDVTFDKATYK
SGEDVRISINNSEVVSSDVTVSHLDQVIYEKKNVEGSELTIPSTVFSPNAGFIVDVLGKK
ADGSQAFHKAAGLAVEDDWTIYPRYGVVAGSKDNHNSITNDQVEGYKNSIDQLANMHINN
YFFYDVYNTPANPFPANVERFTQDWATFLIPNNETDPDKRKSYLPVIDTEAVKELVAHVH
TTGAKAMLYNMIYAVSLEEGVPNEVKSAIVRNLQDHFNFGKKGDVTATDIQQFLDPGDPN
WQKFIIEVMTKAMKEGNFDGWQGDTMGSNWVEKVSEPGKGFSLSEHYPELAAAATEALKK
EGYDFMINDISTGDADRLGKTNVSAPYAEVWGDSIAYDSTYQALTRLTERMRDLYKGKSP
IIAAYTHRGKNASTLSKDNELLTDAIIAASGGYHMTTAALNTSQDAKGFGVIQAEWYLNQ
NLPVNADFANAEFNYQEFIVAYQELLRGRDTLSHDTRRIVDNTNVLVNGNFIGSNPDNAD
EVQPGTVYTITKETKTGDRIAHLINLVGITENKWNSAVNTTEKLTNIQAFVPLGKITKDQ
AEHANIYWATPDAVEGKYNINLRETSANVYYDGGAGQWMAAIDVPSLDVWDMLYVKLNEA
AHVLYQDENGVELERSSDLVGHTGEKINYSTKETIANYLKKGYELVENGFAADGQPNFDR
DANNTNEDGVQEFIVRLTPKIVAFSEAQPIQAGQVVPGDSEGRVYPTPLAPAGQTTADLD
HLSETVTRTVTYVTEDNDGSNRRPAGIADQTDSLHFARKGSINLVTGETKLEDWTAQDGT
SFTALENPVKKGYVVVAEESTDTVSSDLIKAGHHTGIHADVADITDTVIYRPVGAYRISY
ATGKEPTNAQKEITYLNDATNPTAIASPTAILPYVEGLEPKDANGQVLNFVDPLDETKGY
LLPNPESPTADTAISYAISKGSVMVRFLTEGGNTDLQEAQDLATEADFGTKYTSEAPTEI
TKDGRIYHLVGLRADSADPNGTVTKGMKTVIYDYKLATGTVIARFVIQDTSTELQPELAV
ATNVDNGTHYVASAPDEISYDGHIYERVGAAEQTGNVEVGTTILIFAYKVKEIPTPQPSP
ETGSKEIVHPSTSHTQPAVIKEIPSTSITEEDKPLETTSQTAELPEAVPNTPQEGTHLPV
TGEETSHLSLLGLGILAGLTSLVAFKKKHD

Enzyme Prediction     

No EC number prediction in MGYG000002826_00766.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH66	111	716	1.3e-104	0.9946043165467626

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14745	GH66	3.58e-89	202	562	2	331	Glycoside Hydrolase Family 66. Glycoside Hydrolase Family 66 contains proteins characterized as cycloisomaltooligosaccharide glucanotransferase (CITase) and dextranases from a variety of bacteria. CITase cyclizes part of a (1-6)-alpha-D-glucan (dextrans) chain by formation of a (1-6)-alpha-D-glucosidic bond. Dextranases catalyze the endohydrolysis of (1-6)-alpha-D-glucosidic linkages in dextran. Some members contain Carbohydrate Binding Module 35 (CBM35) domains, either C-terminal or inserted in the domain or both.
pfam13199	Glyco_hydro_66	1.91e-84	114	714	1	557	Glycosyl hydrolase family 66. This family is a set of glycosyl hydrolase enzymes including cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.-) and dextranase (EC:3.2.1.11) activities.
pfam17965	MucBP_2	8.58e-14	721	797	3	73	Mucin binding domain. This domain is found in bacterial cell surface proteins that interact with mucins. The archetypal member of this family is the Mub-R5 B1 domain. This domain has a beta-grasp fold.
pfam17966	Mub_B2	7.03e-06	868	914	19	66	Mub B2-like domain. This entry corresponds to the Mub B2 domain. This domain is related to the Mub B1 domain pfam17965. This domain may be involved in mucin binding. This domain is often found associated with the related pfam17965 in bacterial cell surface proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLF55758.1	0.0	1	1195	1	1217
QKI21846.1	9.28e-151	112	715	13	619
ASK26976.1	1.91e-137	139	614	2	462
AWN64322.1	3.35e-80	111	715	161	795
AWN62447.1	3.35e-80	111	715	161	795

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3VMN_A	8.99e-72	111	715	10	635	Crystalstructure of dextranase from Streptococcus mutans [Streptococcus mutans],3VMO_A Crystal structure of dextranase from Streptococcus mutans in complex with isomaltotriose [Streptococcus mutans],3VMP_A Crystal structure of dextranase from Streptococcus mutans in complex with 4,5-epoxypentyl alpha-D-glucopyranoside [Streptococcus mutans]
5AXG_A	1.11e-32	107	716	42	609	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXG_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223]
5AXH_A	8.28e-32	107	716	42	609	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXH_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39653	1.28e-79	111	715	165	800	Dextranase OS=Streptococcus downei OX=1317 GN=dex PE=1 SV=1
Q59979	1.29e-77	132	721	11	603	Dextranase OS=Streptococcus salivarius OX=1304 GN=dex PE=3 SV=1
Q54443	2.06e-69	111	715	107	732	Dextranase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=dexA PE=1 SV=2
P70873	3.19e-16	104	744	31	763	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 GN=cit PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001192	0.997597	0.000608	0.000209	0.000188	0.000164

TMHMM  Annotations     

start	end
21	40