CAZyme Information: MGYG000002827_04759

Basic Information

• Species: Phyllobacterium sp900539805
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales_A; Rhizobiaceae_A; Phyllobacterium; Phyllobacterium sp900539805
• CAZyme ID: MGYG000002827_04759
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
721	MGYG000002827_73|CGC1	79613.06	10.1596

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002827	5102924	MAG	Singapore	Asia

• Gene Location: Start: 1854; End: 4019 Strand: +

Full Sequence      

MMNEPRAKNTLAKKKRKRFKVSPLLGLDAWIDSSLYELRFRLAEFWEDTTIFFRRFHVSG
WRRGVVELLSEGFTWGTAGSVVMLTLAIPAFHDTEKNWRTRDDFAVTFLDRYGNEIGQRG
ILHRSAVPIDELPDHVIKAVLATEDRRFFDHFGIDFLGLSRAMTENLRANSVVQGGSTIT
QQLAKNLFLTNERSLDRKIKEAFLALWLESNLSKKEILQTYLDRAYMGGGTFGIAAASEF
YFGKSVKDVNLAEAAMLAGLFKAPAKYAPHVNLPAARARANVVLSNLVQGGLMTEGQVVG
ARRNPATVVDRARSESPDYFLDWAFDEIRRVGQKLPSHTLVVRTTLDTGIQKAAEESAEY
HLRQFGKEYHVTQAAIVVLETNGAVRAIVGGRDYGESQFNRATAALRQAGSSFKPYVYAA
AMEKGMTPKTMISGGAVSWGNWSPQNYSRQYLGQIDLTTALVKSINTVPVRLAKDYLTTA
PIVALAKAMGVESVISPHKTMVLGTSGMTVMDQATGYNTFATGGLAGVRHAFTQVSDQNG
KLLWDWKTDGNKPHRALTEKAAFNMNIMLSQVPEWGTGRRAALPMTRVAGKTGTTQSYRD
AWFVGFTGNYTAAVWFGNDNFTPTKELTGGILPAMTWQRFMNYAHQNIDLKPIPGIDPAM
PKQDKVAAAKPATADEEALKAAERPRVLSNATVTSLREIGQALKEAPALKLPDVPTKISA
R

Enzyme Prediction     

EC	2.4.1.129

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	122	287	2.9e-65	0.9322033898305084

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	60	702	9	656	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	123	647	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.10e-158	108	685	51	764	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG4953	PbpC	1.53e-100	102	673	33	592	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
PRK09506	mrcB	9.27e-88	124	656	211	759	bifunctional glycosyl transferase/transpeptidase; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRI43828.1	0.0	1	721	1	722
QND54167.1	0.0	2	720	1	715
ATU92358.1	0.0	2	720	1	716
ASV87415.1	0.0	7	719	5	717
QWK78192.1	0.0	13	719	11	717

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	3.67e-76	126	646	39	717	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	2.60e-71	113	644	28	722	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	3.57e-67	124	641	156	690	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.96e-67	124	641	177	711	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3FWL_A	8.20e-64	124	641	160	694	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	4.05e-92	110	652	49	680	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
O87626	7.58e-82	75	653	19	713	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
P38050	2.05e-79	109	630	52	582	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
O87579	2.51e-75	80	661	20	717	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O05131	6.63e-75	75	644	19	705	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000032	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002827_04759.