CAZyme Information: MGYG000002839_01010

Basic Information

• Species: Granulicatella sp001058355
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Granulicatella; Granulicatella sp001058355
• CAZyme ID: MGYG000002839_01010
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
671	MGYG000002839_11|CGC1	77471.27	6.2599

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002839	1704884	MAG	United States	North America

• Gene Location: Start: 24520; End: 26535 Strand: -

Full Sequence      

MKLTGKVTLLTAALFALSAHAVAAETTQTETTVQPTTVEVTTVQPTTETTTTSEGTLPTD
SKNDVDIHVYKPGEEPKYTGLYKADGETYYQVDSKPITNTWKWHGGRWYYFGVDGKMLKS
TITPDGYLVDIEGMLVSPGWNYQGGKWYYALSGGKVFRGDWKKIGGVWYAFHDNGVMYSH
EWSGSYFLKDSGAMADNEWVFDRNYNSWFYIKPGGAYARNEWKGDYYLKGGGYMAKSEFI
YDSKYQATYYLDASGKYVRDQWREIDGKWYHFRKYGEMDTSKWIGSYYVKSDGAMAASEL
VYDPTYNSSFYFDENGKYLNKQWKEIDGDWYYFKDGGYIAKNEWVGSYFIKGNGRMAKNE
MQYDQSTSSSYYFKDDGTYAKNYWAKVGGYWYYFKGNGKVARKEWIENKYYVLDNGKMAT
GTHIIDHYQYVFDGSGNILSKKAVDIGWVEKDGKRYFYNGASQRLGDETTKKVMDISEHQ
GHISNWEGIIKDNDIDAVIVRIGYSGAEDKHLAHNISELNRLGVPYGIYLYTYASTEADG
VEDAEQTIALIKKYNIKPTYPIYFDLEDWRYTNGAKSAPTDTTTWVKIWKAYRDTMAKAG
YTNVRIYSYQYLLQNRLNDPEILKYVDWVAAYTPQLTYQLPYSQPSWGWQYTDKEFVTGL
GNVDMSVWFGR

Enzyme Prediction     

No EC number prediction in MGYG000002839_01010.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	474	659	1.5e-27	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	1.57e-50	471	669	1	191	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
NF033930	pneumo_PspA	5.91e-23	101	354	445	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
cd00599	GH25_muramidase	2.70e-22	472	668	1	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
NF033838	PspC_subgroup_1	2.47e-21	94	354	482	680	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.
COG5263	COG5263	4.87e-21	183	434	60	310	Glucan-binding domain (YG repeat) [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACO16036.1	7.21e-130	182	669	64	490
BBG39499.1	4.07e-129	182	669	75	501
VEE28629.1	4.07e-129	182	669	75	501
ACO20881.1	8.06e-129	182	669	64	490
ADM85180.1	8.06e-129	182	669	64	490

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WW5_A	2.05e-130	182	669	42	468	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae at 1.6 A resolution [Streptococcus pneumoniae R6],2WWD_A 3D-structure of the modular autolysin LytC from Streptococcus pneumoniae in complex with pneummococcal peptidoglycan fragment [Streptococcus pneumoniae R6]
2WWC_A	5.76e-130	182	669	42	468	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae in complex with synthetic peptidoglycan ligand [Streptococcus pneumoniae R6]
4CNL_A	4.86e-08	323	366	5	48	Crystalstructure of the Choline-binding domain of CbpL from Streptococcus pneumoniae [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P59205	1.16e-11	182	369	28	196	Putative endo-beta-N-acetylglucosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=lytB PE=1 SV=1
P59206	1.22e-11	182	369	28	196	Putative endo-beta-N-acetylglucosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=lytB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000841	0.998045	0.000300	0.000314	0.000251	0.000224

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002839_01010.