CAZyme Information: MGYG000002845_00685

Basic Information

• Species: Lachnoclostridium_B sp900543315
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium_B; Lachnoclostridium_B sp900543315
• CAZyme ID: MGYG000002845_00685
• CAZy Family: GT4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
490	MGYG000002845_3|CGC1	57431.54	5.1732

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002845	2730908	MAG	United States	North America

• Gene Location: Start: 82189; End: 83661 Strand: +

Full Sequence      

MNNENMAVKVFRTFKRDGLRGINEKIREHICVKEADLKKESYRDFLFVYSSDCKYEQKER
EIFAGQMKMLELFNYSTELIEEKSFTEDHLRYFKVFVLGKCSEKREFENLFDKIKILNKK
IVLLEELSDFQLYEDEAEYRDKSLSIMAKAKDLIQYYKSVLPHNIGIFLPGLAISGGIRV
ALKHACILQESGKDVTLFTLDSRQKWYDVDGHIFSVINLKETNLYGCIDHAVATMWTTVP
VVEQCSTAVRKSYLVQGFETDFYSSEDPFYVDANKTYSPHISMKFFTVSKWCQKWLAEVF
GQETVYIRNGIELEQFNEKKREFSGKIRILIEGDCEAEYKNVDEAFQIVNELDSQKYEIW
YMSYNGKSKPQYRVDNFLHKVPYEKVAEVYQQCHILLKTSLLESFSYPPLEMMATGGYVV
AVPNDGNAEYMVDEYNCLLYSAGDLDKAKEQIERICQDENLRKKLTENAQKTVAERDWKN
IEPEILAFYQ

Enzyme Prediction     

No EC number prediction in MGYG000002845_00685.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	376	464	2.6e-17	0.55

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03801	GT4_PimA-like	4.63e-24	284	490	143	366	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
COG0438	RfaB	4.60e-18	284	490	153	375	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
pfam00534	Glycos_transf_1	7.63e-15	338	471	13	157	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.
cd03809	GT4_MtfB-like	4.62e-11	284	487	141	362	glycosyltransferases MtfB, WbpX, and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.
cd03794	GT4_WbuB-like	1.67e-09	375	486	272	391	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO35979.1	2.82e-124	139	489	372	722
QBF76212.1	2.82e-124	139	489	372	722
BBF44263.1	6.23e-111	131	489	396	756
QIA33403.1	7.25e-108	164	489	425	757
SET96050.1	3.65e-104	156	490	394	729

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q58577	8.07e-07	277	478	134	335	Uncharacterized glycosyltransferase MJ1178 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1178 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000046	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002845_00685.