CAZyme Information: MGYG000002857_02345

Basic Information

• Species: Hydrogeniiclostridium sp900550095
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Hydrogeniiclostridium; Hydrogeniiclostridium sp900550095
• CAZyme ID: MGYG000002857_02345
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
652		74750.53	5.1891

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002857	2686801	MAG	United States	North America

• Gene Location: Start: 138; End: 2096 Strand: -

Full Sequence      

MEILREPLLQETEISGGMLREYQRLLVEEMLPYQVGIMEKGGALDTIRRAAGYRVPERTE
KEQPTDVGKLMEAMAYGLMLQEDPVLREKLCSLMRLLERVQYADGYLHPSMQAERPADRF
LSLLMSHEMYTLGHYIEGLLACEEALGDGGALRCAKGIGDCLCEWFGKEPGKICGYDGHP
EIELALYRLYRRTGEEKYLSCMRFFVEERGVSGPGHPHFFDWEQERNREKFGKPVLGELS
RWADRHRPFWGRYEYFQAHLPARELREPTGHAVRAAYFYCAMADLAMETGDKELLEACRS
LWLGFEQGNIALNGSIGQDGFWEGIGKAYDLPPDYSYNETCAAIGLFLFGHRMMLQEPDG
RYGDQMELSLYNTVLAGTSLDGKHYFYCNPLEVWPDSLYRYDKIHIQSRRYEWDDCPCCP
PNVARLLGEMGRYAVLSGGESVWVNLYMGIRCTARLDSGPVRLEMETEYPKEGRIRLRIR
DGGRFALRLRVPAWCVEWKLLVNGRPVSAEIEKGFVCAGNGWQAGDTVELQLSMPFRRVY
ADPRAYHLAHQTAVMRGPLLYCAEQADNGRLLSRILLPAASEFEEMWEPELLGGVYTVEA
QAVRAVLEEGPMYRAKAPKREEFRLKLVPYYAWANREDGEMRVFFPEIPAES

Enzyme Prediction     

No EC number prediction in MGYG000002857_02345.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	14	562	5.9e-159	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.38e-141	1	644	1	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	4.89e-116	20	562	10	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAD63750.1	4.81e-155	6	644	3	637
ALA51602.1	4.81e-155	6	644	3	637
ADN02408.1	1.44e-154	12	647	14	642
ADH61394.1	1.50e-152	9	647	14	647
AGB41651.1	2.91e-152	4	647	3	645

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	2.44e-149	6	647	10	647	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	3.01e-144	6	647	10	647	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	6.86e-118	26	644	17	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	9.28e-118	26	644	17	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	2.58e-117	26	644	17	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	3.76e-117	26	644	17	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002857_02345.