CAZyme Information: MGYG000002881_00227

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus
• CAZyme ID: MGYG000002881_00227
• CAZy Family: GH5
• CAZyme Description: Endoglucanase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
366	MGYG000002881_22|CGC1	40183.54	4.3446

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002881	1270591	MAG	United States	North America

• Gene Location: Start: 2507; End: 3607 Strand: +

Full Sequence      

MKIFKKALAAASSVAMAASMCTVSIGSLGTVSAADVSAIDIVNDMGLGWNLGNTFDAWGT
KSYADKSQGYVDNETAWGNVHTTQDIIDAVKAQGFNSVRIPVSWGDNVDASTFDINDKYL
ARIKEVIDYCYNNDMYAIINMHWDWESGGSGFLNQGSAALPQFEAMWSEIATYFSDYDNH
LVFESMNEVTFDYTTLNSFNQSFVDVVRSAGGNNSDRLLVCSGANADLSKTCNSQYKVPA
DDMVAVDIHYYTPATFCVAKQGESWGYSSTWGTDAEKNEMVSNFEKLKTTFVDKGIPVIV
GEYGVLTNEGKDKTSIHEFNKTVASLGKSYDGIATYLWDDSDSGGHMYFSRSKLAWHDAE
IGAIYK

Enzyme Prediction     

No EC number prediction in MGYG000002881_00227.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	73	340	2.7e-87	0.9891304347826086

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	1.05e-46	71	330	13	263	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	1.31e-17	41	312	47	330	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
COG2723	BglB	0.001	53	154	31	138	Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL16523.1	3.76e-101	1	365	1	356
QPB75695.1	5.22e-98	1	365	1	364
AEQ16450.1	7.06e-95	37	361	11	351
CAH69214.1	1.70e-88	37	361	20	360
CAL91975.1	1.70e-88	37	361	20	360

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6XRK_A	3.19e-98	32	361	18	363	GH5-4broad specificity endoglucanase from an uncultured bovine rumen ciliate [uncultured bovine rumen ciliate],6XRK_B GH5-4 broad specificity endoglucanase from an uncultured bovine rumen ciliate [uncultured bovine rumen ciliate]
6XSU_A	9.90e-69	31	357	6	339	GH5-4broad specificity endoglucanase from Ruminococcus flavefaciens [Ruminococcus flavefaciens],6XSU_B GH5-4 broad specificity endoglucanase from Ruminococcus flavefaciens [Ruminococcus flavefaciens]
2JEP_A	1.41e-61	32	352	31	377	Nativefamily 5 xyloglucanase from Paenibacillus pabuli [Paenibacillus pabuli],2JEP_B Native family 5 xyloglucanase from Paenibacillus pabuli [Paenibacillus pabuli],2JEQ_A Family 5 xyloglucanase from Paenibacillus pabuli in complex with ligand [Paenibacillus pabuli]
6Q1I_A	1.46e-57	37	361	13	345	GH5-4broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum],6Q1I_B GH5-4 broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum]
4W85_A	9.88e-57	40	365	7	335	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W85_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W87_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W87_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W89_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium],4W89_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P54937	4.20e-56	37	361	38	370	Endoglucanase A OS=Clostridium longisporum OX=1523 GN=celA PE=1 SV=1
O08342	2.75e-54	32	348	31	376	Endoglucanase A OS=Paenibacillus barcinonensis OX=198119 GN=celA PE=1 SV=1
P20847	4.10e-54	31	361	29	387	Endoglucanase 1 OS=Butyrivibrio fibrisolvens OX=831 GN=end1 PE=3 SV=1
P23660	1.72e-53	35	356	24	350	Endoglucanase A OS=Ruminococcus albus OX=1264 GN=celA PE=1 SV=1
P17901	5.64e-53	39	345	48	372	Endoglucanase A OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=celCCA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000271	0.999006	0.000212	0.000195	0.000151	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002881_00227.