CAZyme Information: MGYG000002892_00532

Basic Information

• Species: Anaerococcus prevotii
• Lineage: Bacteria; Firmicutes_A; Clostridia; Tissierellales; Peptoniphilaceae; Anaerococcus; Anaerococcus prevotii
• CAZyme ID: MGYG000002892_00532
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase C

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
218	MGYG000002892_60|CGC1	25052.29	4.6959

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002892	1231601	MAG	United States	North America

• Gene Location: Start: 3608; End: 4264 Strand: +

Full Sequence      

MKVVKFIGKVLGFIVLAIGIFFAIVYGLIAYQTSRTQISYQDQIYEYSEKYNVDPLLTAS
IIKVESDFDNNAQSHQGAHGLMQLLENTAKHSADVVGIDYYPEKLNDVDYNLDLGVGYFD
YLYRYYNNRDLALAAYNGGVGNVDSWIKEGTLDKDNPDPLNIPFEETRQYVTKVNANYEV
MKKFYKDGLPSEEEISNLRGLSLRNFEIFIKDFIRNIR

Enzyme Prediction     

No EC number prediction in MGYG000002892_00532.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	46	182	1.1e-29	0.8740740740740741

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd16896	LT_Slt70-like	3.13e-62	38	180	2	146	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	1.34e-42	40	180	6	148	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
cd00254	LT-like	2.51e-33	55	179	1	111	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
COG0741	MltE	1.46e-28	27	185	126	287	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
381614	cd16893	1.47e-26	42	179	1	162	LT_MltC_MltE membrane-bound lytic murein transglycosylases MltC and MltE, and similar proteins. MltC and MltE are periplasmic, outer membrane attached lytic transglycosylases (LTs), which cleave beta-1,4-glycosidic bonds joining N-acetylmuramic acid and N-acetylglucosamine in the cell wall peptidoglycan, yielding 1,6-anhydromuropeptides. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACV29351.1	4.33e-154	1	218	1	218
QQN55587.1	3.28e-89	1	217	1	217
QQB62557.1	7.66e-88	1	217	1	217
QQK07141.1	4.59e-48	1	216	1	212
BAG07763.1	3.78e-44	5	218	2	207

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5O1J_A	2.27e-17	33	177	413	555	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5MPQ_A	2.27e-17	33	177	409	551	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
6FPN_B	2.28e-17	33	177	419	561	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	2.29e-17	33	177	423	565	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	2.30e-17	33	177	429	571	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39434	3.22e-16	35	194	475	640	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P0AGC3	4.36e-16	35	191	475	634	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P0AGC4	4.36e-16	35	191	475	634	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
O31976	8.15e-15	40	178	1422	1541	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
O64046	8.15e-15	40	178	1422	1541	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.983887	0.012503	0.000313	0.000073	0.000042	0.003198

TMHMM  Annotations     

start	end
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