CAZyme Information: MGYG000002897_00155

Basic Information

• Species: Varibaculum cambriense
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Varibaculum; Varibaculum cambriense
• CAZyme ID: MGYG000002897_00155
• CAZy Family: GT39
• CAZyme Description: putative dolichyl-phosphate-mannose--protein mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
589		66160.25	10.2012

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002897	2017891	MAG	United States	North America

• Gene Location: Start: 179902; End: 181671 Strand: +

Full Sequence      

MPYPANIEYMQAMPGRPAPPPPAAARQTLTKSNKPKFARARRRPRAPRWTSVTSLPRLYL
RGWRGQQAREEIEKQRAWIVTVIACVIAAIARLVRLGTTHKLIFDETYYVKDAYSLWRFG
YETNWPKGTDAKFAAGDFAAAVADPAFVVHPPLGKWIIGLGMKIFGWSSPFGWRIATAIC
GIILVYLTCRLAWALFNSWILTGIAGMFIATDGVAISLSRVGLLDGVLAVFCLAGVLCVV
YDQLQVRQRLARQLLGPKLGNDLAGMRWWLVAAGVWLGCACAVKWSGLYLLAVCGIFVFL
RDLTLRLSALRISPHSLSKQYPGIKGKQRAWLGAIVRGGLPAFAQLVLVAFAVYLLNWWN
WFTHPGAWGHGKTAAAAEHSSWLDPISDYVTYMSEVMTFHTGVTSKHPYQSYPWQWLINQ
RPTSMLFEKPHGDNGDFTVEAMSSLGNPMLWWVGVIALAVIIYCTVVRRDWRAGVILVGY
LGLWAPWLFYWYRTIFMFYMVVLTPFLALAVTYLIGLLLGLLRPLPQPLLACRDQLVAPP
QRTPDIAVSMGIALVIVILMVAVFFYPVVSGMPIPYSHWGWRMWFSSWI

Enzyme Prediction     

No EC number prediction in MGYG000002897_00155.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	85	362	9e-65	0.9865470852017937

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1928	PMT1	3.73e-17	79	589	25	694	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam02366	PMT	1.03e-10	86	306	5	204	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam16192	PMT_4TMC	1.69e-09	397	586	11	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1807	ArnT	1.00e-07	150	300	64	194	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
COG4346	COG4346	7.70e-07	150	511	136	405	Predicted membrane-bound dolichyl-phosphate-mannose-protein mannosyltransferase [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOX81378.1	1.94e-135	82	589	55	547
QQT14111.1	6.73e-126	78	587	20	545
QQU09498.1	3.77e-125	78	587	20	545
ADI66786.1	3.77e-125	78	587	20	545
QGS11599.1	4.99e-117	78	589	69	581

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P9WN04	2.26e-66	72	588	38	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	2.26e-66	72	588	38	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2
L8F4Z2	1.07e-64	72	588	32	515	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
Q8NRZ6	1.64e-45	78	588	38	519	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=pmt PE=3 SV=1
Q5A688	8.59e-07	82	297	96	298	Dolichyl-phosphate-mannose--protein mannosyltransferase 6 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT6 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000046	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
77	99
170	187
194	216
221	240
334	356
448	467
474	496
500	522
552	574