CAZyme Information: MGYG000002904_02866

Basic Information

• Species: Phytobacter diazotrophicus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Phytobacter; Phytobacter diazotrophicus
• CAZyme ID: MGYG000002904_02866
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
553	MGYG000002904_22|CGC1	62432.75	9.2214

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002904	5266198	MAG	United States	North America

• Gene Location: Start: 42224; End: 43885 Strand: -

Full Sequence      

MTKSSRYGLVVLALFVLYYLIPVEWRLLWQPDETRYAEISREMLSSGNWTVPHFLGLRYF
EKPIAGYWVNSIFQWLFGETNFAVRAGAIFSTALVAGMVSWFAWQLWRDKRVAMMAAIIF
ITMLEVYGIGTYAVLDPMIALWLVAGMCTFWIAVQAKTTSVKAAGYVLLGLTCGMGVMTK
GFLALAVPVLSVLPWAIAQKKWKEVLIWGWLSIASCVLVVLPWGLAIAQHEPDFWHYFFW
VEHIQRFAMADAQHKAPFWYYLPFVLLGSLPWLGLLPGSLRLAWFTRENNRATLYLLCWI
VMPFLFFSIAKGKLVTYILPCFAPLAILMAGYSVQAAERGYRALRVNAWLNIGFGLFGLV
AAFIASPWGPLKNPVWTTLESYKVFCAAMAFFVWALVGWYTLRNTEKSWMLSALCPAGLA
LLVGFAIPNRVMESKQPQFAIGMTKESLASSRYILSDNVGIAAGLAWELKRSDIIMFGQT
GELQYGLSYPDAKDKFIAEKDFNDWLEQHRQEGKVTLVVLLSRGESVNSLPIPPPSNVFV
QGRIALIEYAPQP

Enzyme Prediction     

No EC number prediction in MGYG000002904_02866.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	4	520	1.9e-149	0.9592592592592593

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK13279	arnT	0.0	1	551	1	552	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
COG1807	ArnT	3.65e-97	1	525	2	535	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
pfam02366	PMT	1.33e-36	4	238	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam13231	PMT_2	9.79e-06	62	223	2	157	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBE79197.1	0.0	1	553	1	553
QIH64753.1	0.0	1	553	1	553
QJF18917.1	0.0	1	553	1	553
AUV10176.1	0.0	1	553	1	553
AUU89776.1	0.0	1	553	1	553

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	6.01e-26	4	486	28	513	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B7LM74	4.14e-260	3	552	2	551	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73) OX=585054 GN=arnT PE=3 SV=2
B7N5M2	1.45e-255	3	552	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) OX=585056 GN=arnT PE=3 SV=1
B1LLL1	2.05e-255	3	552	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli (strain SMS-3-5 / SECEC) OX=439855 GN=arnT PE=3 SV=1
Q0TFI5	2.38e-254	3	552	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) OX=362663 GN=arnT PE=3 SV=1
Q0T2M6	6.78e-254	3	552	2	550	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Shigella flexneri serotype 5b (strain 8401) OX=373384 GN=arnT PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.997746	0.001941	0.000209	0.000005	0.000005	0.000118

TMHMM  Annotations     

start	end
7	29
82	104
116	135
176	198
205	227
258	280
293	310
315	334
347	369
384	402
409	428