CAZyme Information: MGYG000002912_01008

Basic Information

• Species: UBA10677 sp900543755
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-552; UBA10677; UBA10677 sp900543755
• CAZyme ID: MGYG000002912_01008
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
617		70620.19	5.804

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002912	1879491	MAG	United States	North America

• Gene Location: Start: 17442; End: 19295 Strand: -

Full Sequence      

MKKYENVSFRDVDLKDGFWLKRYLLNKNVSLESVKNRFEETARFDALRFNYLKNGRKPHI
FYDSDVAKWIEAVAYIMEKDRDSMRENEKFIDELIDCMANAQRDDGYLNSTFQQITPDKI
FRNRGKHELYCAGHLIEAAVAYDKATGKHKFLEVMEKYCDCIYRAFIKEKTAAFVTPGHE
EIELALMKLYRYTGKEKYFEMARFFLENRGKHDEQYVYLDNRYASQDDTDIYNLKEAVGH
SVRALYLYSGVADLAEETGDEKLFKALTSVFDDIVNSKMYVTGGVGSTFRSESFTVPYDL
PNTTAYSESCCAIAFILFALRMRKMDLNPKYGHLIERVMYNSLLSSTDLTGKAFFYENPL
EIALEEYGREVSASPTDKERLPITQRLEVFGCSCCPPNINRFFAELGDVIAFAGNETLYI
EQYVSSDVNTSLGRVEIRENYALSGKAEISSQNFSGKKLAVRLPEWSNKLVATSMGKPVK
YEVEEGYAYFDAEKSFSLQLDFGIKAAFVAANPKVRADAGRIALTYGPVVYCLEGVDNGE
RLNRISVSPEAAEKAKKEKDFHGLYSVKLEGFIDKAESALYFPACDSDREKKSLKFIPYF
AFANRGESDMLVWVRKA

Enzyme Prediction     

No EC number prediction in MGYG000002912_01008.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	12	532	5.6e-156	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	7.83e-137	1	615	3	586	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.66e-114	11	532	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUS96918.1	1.67e-154	1	613	4	624
QHQ63226.1	9.65e-153	7	613	9	621
ACE73687.1	5.14e-129	11	616	9	641
ATG84592.1	6.84e-129	11	616	7	639
QJD87024.1	1.06e-128	7	616	10	652

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	2.24e-130	11	616	15	647	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	5.22e-125	11	616	15	647	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	5.86e-100	7	614	10	623	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WKW_A	2.40e-90	60	617	72	659	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	6.59e-90	60	617	72	659	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	3.91e-89	60	616	72	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000072	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002912_01008.