CAZyme Information: MGYG000002966_02259

Basic Information

• Species: Blautia_A sp900551075
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900551075
• CAZyme ID: MGYG000002966_02259
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
783	MGYG000002966_23|CGC1	87167.69	6.5192

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002966	4084328	MAG	United States	North America

• Gene Location: Start: 45744; End: 48095 Strand: -

Full Sequence      

MKVKDIISKLTIEEKAALLSGKNEWESRDIPRLGVDSITFSDGPHGLRRQEGAGDHLGLN
VSLPATCFPAAATTANSWDPGIGEKIGEALGKEAVAQGVNVVLGPGLNMKRSPLCGRNFE
YFSEDPILAGKMAAAYVRGIQSRHVYACPKHFAVNSREYRRMAMNAVVDERTLREIYLTA
FEIAVKEGKAKSIMSSYNEVNGQYANENKHLLVDILRKEWGFDGIVVTDWGASNDHTEGV
KCQSNVEMPNPGLDSARGLLADLKLGKITEEEIGRAIEPIIEAAIYFKENKQKDFDKESH
HRIAHQAAVESAVLLKNERGILPLKDGTKVALRGPFVNKPRYQGAGSSQVNATKVETIAE
EIKKYNLQVVEDPKAADVVLFFFGLDEIAESEGADRTSMDIPEYQIRELEELAVLNSNVV
GVMSAGSAVKMPWLDKLTALLHGYLTGQAGASALLDLITGKEVPSGKLAESYPYAYADCS
MFNYADEKYRNLQYREGPFIGYRYYDTADIPVQFPFGYGLSYTTFAYDSLILTDEGVRFT
ITNTGMCSGAEIAQLYVGKKETKLVRPKKELKGFTKVWLEAGESKEVEIRFDDKTFRYFN
TKTNKWEVEGGTYNIMIGASCADIRLSSEMEKQGTTDVLPYDIEKLPSYKSGLVRNVHYE
EFQLFYGDVLPEEKVGLLDINDALCQMKYAKSGICRLVYKVLQKKMDKAVEKGVPDLNTL
FIYNMPFRAICKMTAGMVSREMTESIVTVANGHFFRGAAGLIGGFFRNMRANKKYETMLK
NMK

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	29	254	2.4e-67	0.9675925925925926

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	2.81e-78	66	628	118	761	beta-glucosidase BglX.
COG1472	BglX	2.35e-68	30	358	55	390	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	3.14e-62	2	618	52	769	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	6.92e-45	312	522	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	3.14e-35	65	283	85	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFJ55981.1	0.0	1	779	1	782
CBK75766.1	0.0	1	782	1	783
ADL34828.1	0.0	1	780	1	805
AOZ95741.1	0.0	1	783	1	808
QRT31068.1	0.0	1	779	1	807

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	8.98e-200	3	751	5	752	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	4.88e-168	7	642	9	673	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	7.01e-116	2	626	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	1.05e-114	2	626	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	3.69e-95	3	624	6	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	2.46e-199	3	751	5	752	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	7.65e-162	7	639	9	681	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	3.02e-161	7	624	9	664	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
A7LXS8	4.97e-126	1	625	44	738	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1
Q5BFG8	1.32e-110	3	624	11	830	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.987967	0.012003	0.000029	0.000015	0.000009	0.000015

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002966_02259.