CAZyme Information: MGYG000002985_01934

Basic Information

• Species: Blautia_A sp900551715
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900551715
• CAZyme ID: MGYG000002985_01934
• CAZy Family: GH36
• CAZyme Description: Alpha-galactosidase AgaA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
730		84185.55	5.5315

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002985	2543992	MAG	United States	North America

• Gene Location: Start: 6793; End: 8985 Strand: -

Full Sequence      

MAVQFNEDKKIFRLDTKKSTYLMGLTPEGYLGHIYYGDRLYKAAENYMLRMEEPPYTPSV
NKREKSSFLDSFPMEYPTGGIGDYRESCLNVRKEAGQMGCEIHYVSHEIYNGKKALKGLP
ASFGTEEEVQTLDILCEDEILRLQVVLSYSVFEKENVITRSVKLINKGDQKLKIEKIYSA
CLDMDNENFEMLTLHGSWARERHIQQGPLRYGKQMVSSTKGESSHQEHPFVALVTPGTTQ
QQGKVYGMHFVYSGNFIGQAELNQFDSVRTVMGINKEEFGWILKAGEEFQAPEVVMTYSH
EGLGEMTRSYHDFYRNHMIRSKYLHKKRPILINNWEATYFDFNTDKLLDIAREAKSCGIE
MLVMDDGWFGVRNSDNCSLGDWKVNTDKIIGGLKYLVDEVNKIGLEFGIWFEPEMISPNS
DLYRAHPEWAIQIPGREATQSRQQYVLDLSRPEVLDYVYESVASILRSANIVYVKWDMNR
QLSDLGSTYLGAEEQQELFHRYVLGVYALQERLVTEFPDLLLENCSGGGARFDPGMLYYS
PQIWCSDDTDAIERLMIQEGTSMIYPLSTMGAHVSDCPNHTVGRVTPFETRGHVALAGTF
GYELDVTKIPEEDRKMIPEQTKMYHKYHELVREGDYYRIASYRENHLYDCWAIAAKDKRE
VLVTYVHVFSTPNAHSRRIFLQGFDPEAVYVLEGTEEKYTGEMLMKCGFLVKGFWGDFKS
KLYHFVKVTE

Enzyme Prediction     

EC	3.2.1.22

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	11	709	1.7e-272	0.9956395348837209

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3345	GalA	0.0	12	728	4	687	Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065	Melibiase	0.0	289	634	2	347	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791	GH36	1.98e-138	327	626	1	299	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875	Glyco_hydro_36N	3.34e-118	29	284	1	256	Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874	Glyco_hydro_36C	1.12e-24	648	725	1	78	Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL22952.1	0.0	1	726	1	726
ASU28442.1	0.0	1	729	1	729
QQQ93196.1	0.0	1	729	1	729
ANU75639.1	0.0	1	729	1	729
QJU14265.1	0.0	1	729	1	729

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4FNQ_A	1.09e-239	1	730	1	729	Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A	8.14e-237	1	730	1	729	Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
2YFN_A	4.84e-236	1	728	1	720	galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNU_A	7.50e-235	1	730	1	729	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus]
4FNT_A	1.06e-234	1	730	1	729	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9ALJ4	4.46e-236	1	730	1	729	Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
G4T4R7	3.73e-233	1	730	1	722	Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
G1UB44	1.60e-210	3	729	6	732	Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P43467	8.27e-200	3	730	4	732	Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
B8NWY6	5.72e-173	72	722	95	745	Probable alpha-galactosidase C OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=aglC PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000072	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002985_01934.