CAZyme Information: MGYG000002993_01548

Basic Information

• Species: CAG-170 sp000432135
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-170; CAG-170 sp000432135
• CAZyme ID: MGYG000002993_01548
• CAZy Family: GH66
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
539	MGYG000002993_24|CGC1	60329.46	4.766

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002993	2537318	MAG	United States	North America

• Gene Location: Start: 21157; End: 22776 Strand: +

Full Sequence      

MKLICEFDRAQYLTGQEVRLSLPEGVNAPRVCVYQLEAPIDCSVRQNGRTLILPPLPAGS
YGITVEVGSAQWEGAFDVVKDPRQVTRYAFLSDFTSADTETQDVDWIRRLHFNAVQFYDW
MYRHDMLLPPQERYLDPMGRETDLAAIRAKIAACKAAGIRPIAYGAVYAAAKELFAEHPE
WAMYTMDGQAMTFAGWLYYMNIVPSCGWSEHILAEFRKAVAFGFSGIHMDTYGFPKQVWD
AERRPVELTDALPKLIDRAAEAVRTEDPAAGVIFNAVNNWPMEAVAGTRQDAVYIEVWPP
NDRYCDLYTLIREARLCSGKQVVLAAYLHPFQQADTDGAERAFRLSWAAISAAGGTQLVL
GENKAALQDSYYANYASLRAAFLPVVQRYCDFLVRYAALLYLDAGMDIGRTAAGGINEDI
QFEAEDCAFSTDAQADTIWSMIRESGNRLNIQLINLRGNNARWNEAKAAPRAAENIRIQV
RLDRPVAGAFAASPDGESLCAERLPLTCRQTPQGRIYTAELARLDYWTAVWFTMGDEVC

Enzyme Prediction     

No EC number prediction in MGYG000002993_01548.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH66	70	531	9.9e-97	0.8381294964028777

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13199	Glyco_hydro_66	0.0	7	531	1	557	Glycosyl hydrolase family 66. This family is a set of glycosyl hydrolase enzymes including cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.-) and dextranase (EC:3.2.1.11) activities.
cd14745	GH66	2.74e-116	87	396	4	331	Glycoside Hydrolase Family 66. Glycoside Hydrolase Family 66 contains proteins characterized as cycloisomaltooligosaccharide glucanotransferase (CITase) and dextranases from a variety of bacteria. CITase cyclizes part of a (1-6)-alpha-D-glucan (dextrans) chain by formation of a (1-6)-alpha-D-glucosidic bond. Dextranases catalyze the endohydrolysis of (1-6)-alpha-D-glucosidic linkages in dextran. Some members contain Carbohydrate Binding Module 35 (CBM35) domains, either C-terminal or inserted in the domain or both.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUH30411.1	6.56e-109	8	531	17	564
BBB44432.1	4.30e-106	49	531	114	610
AUS97991.1	1.74e-105	8	536	15	574
AHV97051.1	3.18e-103	9	528	15	564
AIQ65315.1	4.70e-103	75	537	16	487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3WNK_A	1.03e-29	8	531	33	720	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
3WNL_A	1.03e-28	8	531	14	701	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNM_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNN_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNN_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNO_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNO_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
3WNP_A	1.03e-28	8	531	14	701	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNP_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
5AXG_A	2.04e-24	75	530	139	606	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXG_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223]
5AXH_A	1.53e-23	75	530	139	606	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXH_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P94286	2.45e-29	8	531	50	737	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 PE=1 SV=1
P70873	5.89e-27	61	531	112	729	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 GN=cit PE=3 SV=1
Q54443	2.27e-09	8	495	111	681	Dextranase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=dexA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002993_01548.