CAZyme Information: MGYG000003004_01267

Basic Information

• Species: Anaeromassilibacillus sp001305115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp001305115
• CAZyme ID: MGYG000003004_01267
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1406		151916.4	4.1019

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003004	2769057	MAG	United States	North America

• Gene Location: Start: 22660; End: 26880 Strand: +

Full Sequence      

MQFKKGSVFTKVLSLTLAIMVSASSLLGGTAFAAETTELDSGRAVNGLSSAANTEAEKDD
FTTVENYALNGSMEEDTNGALTYWTPNLMEGSDDNTIAKATSIRYGDVNIGKYDNYFAAI
RPGSSYVPYEAALEQLLLLPNGTYTLSAYVYSVTTAEDKENGAYAKMYVYEDDGLEAIEI
DLDETSEWTRVVIPDITITNGKCRIGFGLKSVGAGEEEARALAIDDVVLSIPAGTVNGTV
TSPDGDKVANATIQIKDSTDTVASVTTDSEGSYAIDVPAGDDFVVTASKAGFRSVTEEGV
SFVEGEETTLDFQFTEMVEENAYYVDAEFGDDDNDGLSPERAWKTIAKVNETVFEPGDSI
LFKAGCSWEGETLKPQGNGEEGNPIHVGKYGEDDLYPVIHANYVPGTPLSEMETNNKNAL
EITGISHWEFRDLELTNYGIYNDDSTANEQKGRRAVTIRSDGGTMEDVTLDGLYIHDING
WNPKGNEDNGAAISFGANGQTYIDGLTIENCLIKDVTRDGITGGGYTGTRPWGWNSWDKQ
DGVPLRHKNVVIRNNVLDTIAGDGIVPGGTYEVLVEHNLVSHASNNPTPYPNMSGNAKSN
LSAAVWPFDADNSIFQYNEVCYTGVPRGTEVADGEAFDSDYYCVNTLFQYNYSHDNEGGF
LMLCGPAYAYNDGTVVRYNISENDGSLYGKRTIFEIGGGGGVDHSYIYNNTIYTSEDHSV
FSVMRGEPWDGKPKGTNFINNIFAINGNVAQFGFAGDPRDKGKEGIVTYDHNLYTGTLFE
GTLKDMPEDKNPVFGDPKFVDPGNAGDGYENAKAYQIQEGSAAIGAGMQIDQSWLGQKLD
TMSYTAGDGSTAENKAVYFHVDGEEFQWQNPNGGIDFFGNPLPTRSAPDIGAHQFSVEPP
MPEEHTLTVQFDLTAQMVGEGMDIEIANKVGSYQTQVMGGDPITFTFVPTAKGREFRSIT
VNDQPVIFDEDTDTYSYTYEGTMDNQDMNLVFHFEVVTKQALVTAIEEAQALVGGDEYNT
VIPSIRKLFDEALASAKVVRDNKQATQDEIDAAIEQLLKIIPFLSFTAGDTTALESLLAV
VDKLDEANYTASSWATLQEKVAQATTIVEKEEPLKVEVDEAYEALYNALTSLEYKADTST
LEAVVARALEIEAVLKTDYLEVGQAEFIAALRFAQQILQKPEPTQQEIADAADALNLAMA
NLRKIPDKTALESLVKELSAISGDGYTAESFAALRAALNIAQQVLANPEATQEEVDSALS
TCKDAKEKLNKVHNSSSGHHSSNSGNSSSSGNISGNGTAVAVPSTIVAAAQGVSAQQAYV
RSDTTVNFTLKHGQAYCFKMTVVNGNNQMPSFTVGNGSVLKTQFVAKVGNDYYYRVYATG
TPGQSTGVYTTLPGQNAVKHCTVTVG

Enzyme Prediction     

No EC number prediction in MGYG000003004_01267.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	321	893	2.9e-119	0.9918533604887984

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13620	CarboxypepD_reg	7.95e-16	235	313	1	80	Carboxypeptidase regulatory-like domain.
pfam07554	FIVAR	1.54e-05	1209	1270	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam13715	CarbopepD_reg_2	8.98e-04	236	316	1	78	CarboxypepD_reg-like domain. This domain family is found in bacteria, archaea and eukaryotes, and is approximately 90 amino acids in length. The family is found in association with pfam07715 and pfam00593.
pfam07554	FIVAR	0.001	1000	1061	1	65	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
COG1196	Smc	0.004	1000	1271	216	446	Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQQ09247.1	9.00e-80	323	840	20	498
ARN57216.1	8.18e-77	323	840	20	498
QNM84309.1	8.18e-75	321	821	10	467
QDS99205.1	4.81e-74	323	898	30	513
QUE53042.1	5.13e-74	318	892	18	490

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7V6M_A	5.03e-48	316	894	4	576	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
5GQC_A	1.21e-42	323	898	20	600	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]
7V6I_A	1.52e-41	323	892	16	606	ChainA, Lacto-N-biosidase [Bifidobacterium saguini DSM 23967]
6KQS_A	1.57e-28	321	684	245	617	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]
6KQT_A	1.57e-28	321	684	245	617	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	1.33e-09	1067	1258	1664	1864	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000511	0.998538	0.000234	0.000253	0.000227	0.000200

TMHMM  Annotations     

start	end
12	34