CAZyme Information: MGYG000003004_02162

Basic Information

• Species: Anaeromassilibacillus sp001305115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp001305115
• CAZyme ID: MGYG000003004_02162
• CAZy Family: CBM51
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2055		224109.02	4.1234

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003004	2769057	MAG	United States	North America

• Gene Location: Start: 11021; End: 17188 Strand: +

Full Sequence      

MVEKRMEKIMKRKTVRSFIAVALALQMAAAGATPVFAAQAAQAVPAARQAITAVVQEEEG
DIALYLSDMKPTKTKVGFGSLGVDQDIDNKPLRLLDENLDIVEYEKGLCAHAASELVYDI
SAQGAKTFQAYIGVQADSVYDGIYGNCGFTVEVDGEQKYHIDDLSGQAAQVFVNIDIPEG
AKTLTLKTDNGTDGVTTCDHSLWCDAKILCDREIQTTLASAAVTAQQSVLPVGKTTQLTP
VGYSVGGAEIPADTLAVSYKSSDDSIATVSSSGIVTGIGDGIATITCTLTQGDVVREATV
DIIIGKGDPASSWQIASPDGSITALFALHDGKVSYSSFKDNNLVVDNSAVGLTTSLGDFS
ELTFVDRVDEEINDPYELIGAKITQVENHANQTTLNFTKEGVEGVTFSIVTRAYDDGIAF
KYQITAENEDTPITISSENTTLQIPQGSTAYVMPYINHNEQVEEEKQLSELDERYCMPLL
YKTPTGEYALISEAGLHDEYCGADIIGNPNGRLDIVFSNEQKSAIQTKAPFATPWRFAVI
GTTADIVENTMAENLSPANVLEDTSWIQPGVTAWTWLNRDPVNDPDVYKEYIDLAAEMGW
QYLLLDEGWQPSGSSQGHSEFAYYGYYDWTEDLIDYANEKGIGLIAWQNHNDLKKGDEAE
RRIKELADMGIKGIKPDFFNSQSQDYIQFYNRLMRVTAENHMFINIHGANKTTGERRTYP
NALSREGIFGAEQDLFRPADVSAYHNCMLPFMRCAVGPADYTPMFSHRTNTRKQFTIAQM
GALTVTYESGIQCFADRPEAYLNSPARYFFEDFPGQWDETQLLAGEPGEYVNTARRSGEN
WYLGLICNEQRTAEFQLDFLGEGDYYAFIYEDGETVDDITARIEKVDKDTVLTIPMAEHG
GAMIKILRNQPSQAESITLSASELTLEQNDSTVLTATLTPEDVEYDTVTWTSSNPEVATV
ENGKVLGLKPGVTTITAATGFDGNITASCEVTVRRPEFSLTNDWSIIRNDPENWKLTGVN
SITITTKAGEFYSGTDNAENVFLTPVEGDFTATTKLTFVPGANYQTAGIIVYQDSKNLYG
AYKRYHSGYNGNIFTDFAINKGSASEHTTPDLNKDAPVYLKVVKEGTTFTAYYSYDNSEW
VQIADPLNVSSLTGDLKIGLYAVDGNGKSGSLPATFEDFTINNEVIPFANQYVEATEISL
DHTELSMKQYETATLTATVTPEGADDPVVWTSSDPDVASVDENGKITAYQAGTVTITAMT
GFNNEVTASCEVTISPADLVLTDDWDVLRNDPEHWQINSENSITIITQPGEFYTNTDNAK
NVFLSPVEGDFTVSTKLDFVPTDDYQTAGIIVYQDSSNIYGAYKRYHSGFDGNIFTDFVL
NNGKFSEHTTPDADPEASVYLKVVKEGTTFSSYYSYDNQNWTPIADPVDMTMGGKVRMDG
DLKVGIYAVDGNGKTGSLPATFEDFTVNGNVGSFAEEPAVEESTSFMVNYGTNVELTLNG
EEQTIANLIGRYTGNFEEGTELNLSFVPSVEGREFSEILLGGEPQKITNTKEFTYTAEKG
ATAETLDFSFTIVNKEILNSLIAYADTLDNEVAAAVPTVQAKFEKAYHEAVRVAKEVAVK
QTDINDAWGGLLDAIHYLSFQPGDKSELEIQLEIAEAIVEASEGLDPSTIEELKDAMEEA
QKVYDNPDAMEQEITEAVHALKEAIDNVRYQADKSILATVLAKAEAISNELDLYVEEGKE
EFLTARKEAQEVYEDPQATQEEVDNASKTLTAAMTALRKIANKDYLKELMEIAQAKDADR
FTVASYQNLVKVMQEAQVVLDDNTLAEGDPAAEAKIRNIETKLAAAIDHLDEKGSSSSGG
SSSGSSSSSSNNDSTSTATTASNFTLDESTKSCSFRVGERFVFTVKTTSATAPTVTANNA
NVSVQYLTKTADGYSFQLNGAGMGNSTITVTLDGTSSTFTANILEGSVHSDTTGSISIKK
GQAYTFKMTVTDGSTSTPLFVSGTDGVFKTQFVKKDGNDYYFRIWATGNVGSASGIYTTM
PNQNAVRHCVATITA

Enzyme Prediction     

No EC number prediction in MGYG000003004_02162.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	311	906	5.2e-166	0.9793977812995246
CBM51	65	208	1e-28	0.9850746268656716

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	4.34e-74	563	814	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	6.93e-46	317	557	1	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam08305	NPCBM	1.34e-29	65	208	4	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
pfam14509	GH97_C	1.80e-29	817	906	1	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.
pfam17851	GH43_C2	9.31e-21	1330	1468	59	203	Beta xylosidase C-terminal Concanavalin A-like domain. This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUO18240.1	7.26e-145	304	905	21	613
AEE97714.1	1.20e-123	313	911	12	595
ATB36826.1	6.40e-116	308	906	20	589
ADO69318.1	1.46e-114	302	906	15	591
ALV48579.1	3.91e-94	309	1006	37	701

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	2.29e-68	317	906	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	2.51e-67	317	906	20	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5XFM_A	2.50e-61	315	903	19	639	Crystalstructure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_B Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_C Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_D Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron]
5HQ4_A	2.11e-54	315	905	3	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	2.11e-54	315	905	3	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D7CFN7	1.08e-83	311	901	33	615	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
Q8A6L0	2.38e-68	313	906	23	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	3.71e-38	314	877	24	688	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
P33747	8.86e-11	1184	1275	26	118	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2
P35838	9.47e-09	1123	1274	165	316	Uncharacterized protein CA_C0552 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_C0552 PE=4 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000716	0.998134	0.000266	0.000411	0.000249	0.000202

TMHMM  Annotations     

start	end
19	41