CAZyme Information: MGYG000003004_02308

Basic Information

• Species: Anaeromassilibacillus sp001305115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Anaeromassilibacillus; Anaeromassilibacillus sp001305115
• CAZyme ID: MGYG000003004_02308
• CAZy Family: GH97
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2496	MGYG000003004_50|CGC1	273813.53	4.0378

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003004	2769057	MAG	United States	North America

• Gene Location: Start: 1559; End: 9049 Strand: +

Full Sequence      

MKKRAWRSLLSLVLAFTMVFTIWTPALAKSEAAGEWDPSAEYKLLTVLKDAGIEVYSGDT
NPGAKVDLWWNYEGDALCWRLIPDDEAEYYRIQPKNTNATVLMPKESNAVAGTALTLGNI
DDADDSQWWKIVPTDQDGEYMLLNKASEGSAEAYIALENDGVNDGTLVILSDGTTASSYW
KVSKVEVAPPEPVDPVVNVSAGKTLLDVDESTSVSATAFDEYEEQIAVTEVKSSNPEVAE
VTSSEGIYTVTAKANGVASIVASATLSDGTKVEGYTPIIVGTEPVDGAIYQLTAPKTDVS
ALIAYDEAYGAVSYAALSDNSVVVSASPMGIVTSEGDFRSGLEFVDVEESNGTEEYELIG
AKVKYVNAPYKQMTFQFEKAGVPYAIILKVFDDGVAFRYSIDGDENTDLSISSEHTGVRL
PDGAYTWAMNYSQSNEDVEIEHASIKELNYQYSMPLLYETEDDVFALVNEADLNGTYCGA
QMTGDGTGLLNIVFTPEQTDDVETTLPFASPWRYVVIGDLQTITNNTLAESLAPASKVED
TSWIEAGTVDWTWLNGDLRHDQLPDGVSFETAGLEYYKKYIDFAVEMGWKYQLLDEGWQP
RGNTAAGEHEYEGYYDWMPDLIEYAAEKGVGLIVWARKANLTDDDYRQKLFKEWADMGIK
GIKPDFFDSQDQETIQLIEKMMAETAENHMLINVHGAGKPTGERRTWPHAIAREAVRGAE
AYAVDNIETWGAVMDAHHNCSLPFVRGAVGPMDYTPMVSYGANDNLTNHNPGRFTVAHMT
ALPVIYECGLQCLADKPDVYRAHPGYEHYFKNMPSEWDEGILIDGYPGDYVNMARRNGDD
WYQGIICDGKLDATFSLDFLGEGEYVAYIYKDSPEVLAEGPAAYDQFGRHDQDGSNLVVD
QATVDSSIVEEIKVVTKEDTLTIPLAETGGAAIHYVPRDQVEEEIPAVSEAKRITDRLIE
LDWNQDVTGADEKSSFKVMVNGQEAALNDYGYNAGNGALYFDQMTTLELKEVPAQDAQIT
VQVVGSVTNEMGEAADTETVYAVVAAPFYTQSVKADCGITVKSSDAVEKSTLQAAADIVD
VMLSARSDVVEKMIENGAELAVYGKGESAYSIPEHRATYDPDMYYVEGYGGMVGNPVASI
YDANVLRLLQKDSPDHYTGYENESVLVHEFGHSVHLLGVDSIPELKEEFDAVYASAKQKN
LWPNSYAISNREEYFATCTAIWFNVMSESADGSWDGVRGPVNTREELKAYDVDMYNFLAK
IYPETEAVSDAWADVPDNFHPGEEPEEPEWDTEASYKFRTVLNDSCIESNSNNSSAGAII
DLWWDFDGEAQYWRLIPDSNNEYFMIQLESASSTVMMPTDSNAAEGVALTLGNIDEENDA
QWWKIVPAEQDGQYRLMNKASEGSPLYIALQNDATESGTLVILAKDTAPSSVWVVEKQEV
NENVHKADPDAIYLSDLEWLNAVDSWFLVRKDQTVNQTEISWNENGQTVIYEKGIGTFAP
SQVTFDITGLHAETFKAIGCFPAGAGSCEFIVLADGEQILSKTLSYDGNQISPDNGALEV
AIPEGTKILTLYAKGSSSTTWADARLYCDKEAQKDLARVTIKAEETHLQKGASSAINLTG
YIGQVGWGGMQNIEADLTNAIITYSSSDESVAKVEDGKIVAVSDGTATITCTVELDGVKQ
SSTLDIIVGNGNENSWTVVSPDGNIETLFTLRDDGSVVFTTSRNGNTVIGTSPTGLVTNL
GDFRTGLTFVDSKTETVKDSYDLIGAKKSHVDAIGTELTLNFEKDGVTYRIISRAYDDGM
AFQYEIEGQDGEELTISSEATGFLMPDGSVAHTMDFDKANEERYYQKEIEELEGGYCMPL
LYETPNGDWALLSEAALTPQYSGGQIVGHDTGMLDVVFSPEQFSDVVTTAPFTSPWRFMV
IGDPADIAENTLAETLSPDSVIEDTSWIVPSPVDWTWLNGDLRHDNLPEGTTWEETAPKL
YKQYIDFAAEMGWKYQLLDEGWQPRVYNRGNDLYDGYYDWMQEVLDYAESKGIGLIVWAN
KRDLDTPEERERIKEWAEMGIKGIKPDFFDSQSQEIIQLLDILTKEAAENHMLINIHGAG
KTTGERRTYPNAICREAISGGESCVNDAYFDCMLPFTRFAVGAADFTPMASYGNTFGMKS
VTQAHLVAQTIMFECGIQTMADKPDVYRGHVAYENLFKNLPTSWDDSILLDGDPGHYVNM
ARRTGDDWYVSIMGNEARIAEFDLDFLEDGATYTAYIFRDSDDPTNDDSNKAIITETKTL
TKGQTMSIDIKATGGAVVKIIKNAEQTDWTELKAALDEAKSLDSSKYTAASWNVLETAVK
AAEQLPADADQAAIDASVKAIKDAIDKLVRKSSGGSSSSSSSSSSANNTMGSDGVVVAVG
TGAATAQPKVVSDTTVDFTVKSGSAYCFKMTVVNGNNLMPSFTVGNGNVLKTQFVTQIGN
DYYFRVWAVGTPGQSTGVYTTLPGYSAVKHCTITIG

Enzyme Prediction     

No EC number prediction in MGYG000003004_02308.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	1694	2300	5.2e-155	0.9793977812995246
GH97	290	933	2.6e-151	0.9730586370839936
CBM51	1453	1586	6.7e-17	0.9402985074626866

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	3.87e-67	540	804	1	268	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam10566	Glyco_hydro_97	5.07e-54	1944	2190	1	267	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	6.55e-49	1700	1938	1	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14508	GH97_N	1.18e-36	310	534	16	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	6.84e-29	2204	2301	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUO18240.1	5.79e-128	1693	2307	27	621
AEE97714.1	9.79e-115	1692	2298	8	588
ADO69318.1	9.81e-100	284	951	19	602
ATB36826.1	3.14e-99	284	951	18	600
AYB46145.1	3.86e-97	1700	2264	37	582

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XFM_A	6.97e-59	1698	2295	19	637	Crystalstructure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_B Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_C Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron],5XFM_D Crystal structure of beta-arabinopyranosidase [Bacteroides thetaiotaomicron]
3A24_A	8.69e-56	370	880	80	610	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	8.87e-55	370	880	94	624	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQ4_A	2.61e-38	1697	2299	2	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	6.20e-38	1697	2299	2	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D7CFN7	2.57e-83	296	937	43	625	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
Q8A6L0	6.88e-55	370	880	101	631	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	2.56e-36	285	884	18	696	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000326	0.998898	0.000190	0.000212	0.000177	0.000159

TMHMM  Annotations     

start	end
5	27