CAZyme Information: MGYG000003009_00673

Basic Information

• Species: Oribacterium sinus
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Oribacterium; Oribacterium sinus
• CAZyme ID: MGYG000003009_00673
• CAZy Family: GH36
• CAZyme Description: Bifunctional alpha-galactosidase/sucrose kinase AgaSK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
755		85571.48	4.9613

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003009	1309457	MAG	United States	North America

• Gene Location: Start: 79; End: 2346 Strand: -

Full Sequence      

MAIVYDKEKGLISLHSRNTSYQMGIGPYGHLLHYYYGKRGEGDFSYLFQYKDRGFSGNPY
DANADRTYSMDTLAQEYSSFGNGDYRIPACSISGSDGEGILDLRVDRVEIFSGRSHLEGL
PHAKAVEECETLRIFLKEERKRVELELDYILYPDEDVIVRNARIKNISEEALFVERLDSL
SLDFSYGDYDLIHFSGRYGMERLYRREPLQQGVKHIASMRGSSSHQENPFGILAGRNTTE
HSGDCYGFALLYSSSFHLDVERDSYRGTRVSLGMQNDNMHYLLEAGEELQSPEAILSFGD
GLSALSNQLQSMVKKYIAATSPLPYFPILLNSWEACYFNFTGEKIIELAREGKALGMNLL
VMDDGWFGKRDTDFSGLGDWVTNEEKLGMSLESLGHRLEEEGMHFGIWIEPEMVNEDSAL
YRAHPDYALQIPGESPIRSRSQLVLDFSRKEVVDAVLESLISCFRSVPLSYIKMDMNRSI
MDVYSHGVNFQGRGKILHRYCLGLYRFLSGLKEAFPNVLIEGCSGGGGRFDLGALYYTPQ
IWLSDNTDAIERLEIQYGSSFLYPIQCMGAHVSQVPNHQTGRVVPMNTRAIVAMSGSFGY
ELDPKDCREEDRAEIHKQIAVVEELESLMKEGSYYRLSGYGETGGYTPQSEDRVSGKRFT
AWSFVSPDKKKASLSVVWRDKNFNGANEILYCKGLKREGKYRLRLLLQGGFFQDEAFVES
LLERTYSGFSLMEVGLPLPEGKGEYDSFLLLMEEV

Enzyme Prediction     

No EC number prediction in MGYG000003009_00673.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	12	734	2.2e-228	0.9912790697674418

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3345	GalA	1.76e-157	11	736	3	665	Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065	Melibiase	7.88e-153	289	632	2	347	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791	GH36	1.00e-123	325	620	1	295	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875	Glyco_hydro_36N	1.99e-85	29	284	1	256	Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874	Glyco_hydro_36C	9.08e-12	660	752	2	78	Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VCV24080.1	1.68e-249	1	755	1	750
CBL13466.1	4.38e-246	1	755	7	756
CBL10229.1	4.08e-245	1	755	1	750
CBL26995.1	1.89e-235	1	748	1	722
CBK83446.1	3.15e-234	1	748	1	723

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2YFN_A	1.90e-226	1	755	1	720	galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNQ_A	5.24e-187	1	755	1	727	Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A	9.38e-185	1	755	1	727	Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNU_A	2.98e-183	1	755	1	727	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus]
4FNT_A	4.20e-183	1	755	1	727	Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G4T4R7	1.08e-222	1	755	1	720	Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
Q9ALJ4	5.13e-184	1	755	1	727	Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
G1UB44	1.42e-172	3	745	6	721	Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P43467	7.06e-164	3	676	4	667	Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
P27756	1.41e-135	29	637	26	633	Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003009_00673.