CAZyme Information: MGYG000003014_01178

Basic Information

• Species: Actinomyces graevenitzii
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces graevenitzii
• CAZyme ID: MGYG000003014_01178
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
373		39233.45	8.5773

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003014	1770918	MAG	United States	North America

• Gene Location: Start: 19288; End: 20409 Strand: +

Full Sequence      

MTLTPAQEVAQKLAQLEAHSQASADDAAPADAVAPAHAVAPAEAATTGAVAGTPAHDDAS
SQPAAQARQPGEQAQQPGNNCCAASEYLPDRAVAYAVDQRVQYADYFGKLPVAVIIPAYN
EAERITATVQAALAIAGVERVVVVDDGSTDNTAKIAKRAGAEVISKPHKRGKGAAMGSGV
AYLRKSAQPDADYLLLFVDGDLGASASACEVLIEPVASGRCSMSIAVPPAFKGAGGHGFV
LGTARKAISEATGWEPQAPLSGQRCMNSFVFEACKNQLSRGWGVETWLSVKALEAGFTVL
EVPCSITHRVSVNNLAGRLHRLEQFRQVRKTTRKLRPSLVSRLLSKRRSQANVNQAVASQ
APFEPYRMLRPTA

Enzyme Prediction     

No EC number prediction in MGYG000003014_01178.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	114	236	4.1e-21	0.7176470588235294

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04179	DPM_DPG-synthase_like	3.42e-28	114	292	1	185	DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily. DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.
pfam00535	Glycos_transf_2	6.52e-20	114	237	2	124	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd04188	DPG_synthase	7.21e-19	114	226	1	117	DPG_synthase is involved in protein N-linked glycosylation. UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate.
cd06423	CESA_like	9.85e-18	114	201	1	88	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
COG0463	WcaA	5.93e-17	112	202	5	94	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHO90182.1	1.17e-64	113	367	2	251
CED90459.1	4.75e-63	115	373	2	255
QPL05503.1	4.24e-62	113	370	12	262
VEG73787.1	1.38e-61	112	369	11	262
AMD88414.1	1.70e-61	115	369	1	249

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003014_01178.