CAZyme Information: MGYG000003035_01725

Basic Information

• Species: CAG-485 sp900542185
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900542185
• CAZyme ID: MGYG000003035_01725
• CAZy Family: GH81
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1594		176744.55	4.546

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003035	3548444	MAG	Netherlands	Europe

• Gene Location: Start: 21130; End: 25914 Strand: +

Full Sequence      

MNKRNSLLLALGVAVTMQCAGQTVVHPGDKKGSYASYTPLNLCKSEDHVPGDYGFQGDQS
RLMQTRRLWIHEREGQPIPTNDWWTNLITQPYSGRMWSYPQFVQAQNYGVDVQAPSFWIS
DGTEMKSNTTVSVRGENFYPESAVAEEWHDWDVQFEMRDGNKRMYTTMAHGIPFTWIETE
GIDPTVTISRSYQASAEFIATDARVMDTEGNTVNGSVESDCLILTLGSDLYAVYMPAGTS
VTVRGGTMSLKFNGRRQFVVIGVIHDVEDAVTMKPYACHVPRSSRVDYTYTPGKLKTIWH
IDTEDLNSESGAVAALPVMQGFLPHHYRDTGNQPGFAFNGITYATPRGKLKMATGNDFEI
TYDFYGMLPYYAVPQSGDSSEHPYDAARMKEMLSNYAAKGSFGDDTYWGGKGLSQMALNM
MFAREMGETELFNTCRKRLKDALVNWLTWTPGEQRNFFARYDRWGGMVGYATSYDSDTFN
DHHFHYGYFTYAAALLALVDDEFRDNYGDMLTLIAKDYANWDREDTDFPLYRTFDPWAGH
SFAGGMGDDNGNGQESTSEAMQGWGGLYLLGVALRNDAMRDAGIFGWVSEARGTAEYWFD
RHSDPDTGKPGYHSSASDDYNINYDLFRKDGKTHPYNSNLTCHGVGFWTYFGYDAIFMQG
IQWMPISPALDYLSEDKAFAKWDWDRMWSDKSIGGWLASDRTDAGYLSDSGGWGNVALSY
MQRSDPEGAAAVFDQCWEAREPEFYTYDTNGISYFVTHSHLSYGDIDWSIHASIPTARVY
VRNGVKTYMAFNPGDNAVNVTFSDGFRLEDVAPRRLKVSGVESLDNTSITTAEDVKEDPR
DRIEMLNLALRKDVTTSGNENDGFLPTYITDGDHSTRWASLKEDNQWLMIDLGKPALLYQ
VRIHWETAAPYDYKILFSTDKDVWTEVADVPNCAGGTETIATDDMTARYVKIQCGKRRAE
TWGVSLFEVEAFGCYEDVPDSELLGMMIEAEKDVLKQHEAARVRATGLTKGLQWVDVTPQ
WSCAADQGEITADGMFTPAVYPKATVSAEVNGVSVSKGLIVEEAIYPRYLSLSPQSCVMA
AGYDMEPELEVLDQFKGAMDHNQANLIWNVTDREGNPSQGVTVDGTVPRLHAVSCGEYIV
TVANPAGTLSASIDVTVKKVDEINLALGIAVGGEARARATSSTNDVAVRTAEDAIDGDNA
TRWEAPASEILNESDVTWTLDLGSSRDFNTIVIQWENAYSSDYDIAVSDDGTTFNVIKEV
RGFNAAAGLTQVLQPGARNARYIRFINRGRGTAYGVSFYEFMVYNLTEGLQLTEMSLASD
FAISPAGGKLNLTLEGMNQLGIRHPVGDVTYELAEADVSKASGNPGTIEGNIYTAGDPGQ
YTITAVSTSGVRSNPLNVHVFEGEKIVIDPANVTVHDGTRDDGMSNAFDEDINSLWVMRD
HPGTRDYETGFTVDLGKAYRMTALSLAFEGACSQSYIIDFSADGRSFDTPFEYNGSEGIK
ELRERSLHSTAPVRYARFRSTKAATDYGVKLRDFSLYAIPANPTGLNTVTGNGNALHAPY
TVCTVDGRLIVYKGARLPKLSPGIYLINGKTVRL

Enzyme Prediction     

No EC number prediction in MGYG000003035_01725.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	65	735	7.3e-100	0.9565916398713826
CBM32	855	970	5.3e-23	0.9354838709677419
CBM32	1178	1302	1.6e-20	0.9516129032258065

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5498	Acf2	3.41e-42	77	754	89	745	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
pfam17652	Glyco_hydro81C	1.39e-36	404	696	29	309	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
pfam00754	F5_F8_type_C	3.32e-16	1179	1300	2	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	1.30e-14	854	969	2	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	5.26e-06	1166	1289	2	130	Substituted updates: Jan 31, 2002

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACU60275.1	6.30e-157	5	973	7	1042
ASZ13836.1	2.09e-147	22	973	21	1041
ASZ13602.1	2.29e-129	6	991	10	1063
AXT55622.1	2.45e-114	6	991	12	976
AIV39518.1	9.21e-106	138	1544	336	1687

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FOP_A	6.85e-75	78	819	35	714	ChainA, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	9.09e-60	31	809	8	741	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5T4A_A	1.06e-59	31	826	28	777	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125],5T4G_A Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5UPI_A	2.23e-59	31	809	8	741	ChainA, BH0236 protein [Halalkalibacterium halodurans C-125],5UPM_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPN_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPO_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125]
5T4C_A	2.56e-59	31	826	28	777	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9UT45	6.55e-17	322	733	323	711	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
D4AZ24	1.21e-15	407	731	574	891	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
Q09850	1.76e-13	323	559	288	537	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
Q5AIR7	4.08e-13	323	669	721	1066	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
Q12168	3.39e-11	385	581	430	635	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000844	0.356466	0.641865	0.000354	0.000260	0.000190

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003035_01725.