CAZyme Information: MGYG000003084_01015

Basic Information

• Species: CAG-724 sp900551005
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; CAG-724; CAG-724 sp900551005
• CAZyme ID: MGYG000003084_01015
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
593		69137.99	5.0673

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003084	1926204	MAG	Spain	Europe

• Gene Location: Start: 15224; End: 17005 Strand: +

Full Sequence      

MINLLYAGNSGVFDGLIIGALSYIKHNQCAAHVYILTMDYREKNPRFTPITEEDRAFLEK
IYRRANDDSRVYLIDAGEYYKNEMSASPNGETGYTPYTFLRLFADLISEIPEKTLYLDTD
TLIAGDISELYEKDISEYELACVLDYYGHHFLGYHYFNAGVLLLNMKKIRESGLFRRAIA
MCAKKKLFLPDQTALNRLVRKKLLLPYKYNEQKHFPDDTVIQHFSKTILWLPYFHTRNIK
PWQTEEVKNTLTRKYDDILEEYKNMKYIHEKELRRCEIPVFFACDDNYLPYLAVALKSLI
LHADDSRKYHIYILCDSVKEENAAKITAMATANVAVEFVSVTEKIAPILGKMKLRDYYTP
SIYYRIFIAKMFPELNKAIYIDADVVLNDDIANLFDIDLGDKLLGVIYDAIVSSEQVFRD
YSELGLGIPYEKYFNSGVLLMNLHAIREYDVEGKFLYLLNRYHFNTICPDQDYLNVICRD
KVLYLDEGWDKMSLNEDYPGTPSLIHYNMFFKPWSYDNVPYERYFWKYCAGTPFEAEIKA
AKAAFGEEERKADEEGGRNLRAQAAAICDDENNFRRVLCDENEEKGAEELLAC

Enzyme Prediction     

No EC number prediction in MGYG000003084_01015.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	259	517	5e-56	0.953307392996109
GT8	3	244	1.5e-34	0.8715953307392996

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	4.93e-66	278	514	1	246	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
pfam01501	Glyco_transf_8	2.79e-50	279	517	1	251	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
COG1442	RfaJ	2.57e-45	278	527	3	258	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
cd04194	GT8_A4GalT_like	6.00e-36	2	244	1	248	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
cd00505	Glyco_transf_8	2.94e-23	280	516	4	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR72462.1	2.50e-138	3	569	2	578
AEE15925.1	6.69e-85	1	276	1	277
QAT42779.1	5.88e-77	278	577	8	311
ANZ59860.1	1.03e-76	278	567	6	297
SFV41589.1	2.52e-76	277	545	7	280

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U4B_A	1.97e-21	278	538	302	592	ChainA, WbbM protein [Klebsiella pneumoniae]
1SS9_A	4.06e-19	280	495	3	213	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	4.43e-18	280	495	3	213	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]
3TZT_A	1.00e-12	285	487	13	210	Thestructure of a protein in glycosyl transferase family 8 from Anaerococcus prevotii. [Anaerococcus prevotii DSM 20548],3TZT_B The structure of a protein in glycosyl transferase family 8 from Anaerococcus prevotii. [Anaerococcus prevotii DSM 20548]
5GVV_A	1.27e-06	98	200	87	186	Crystalstructure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae],5GVV_F Crystal structure of the glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P43974	6.66e-22	280	505	41	260	Putative glycosyltransferase HI_0258 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0258 PE=3 SV=2
P19817	2.66e-16	280	520	28	277	Lipopolysaccharide 1,2-glucosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaJ PE=3 SV=4
Q9AEU2	1.26e-11	361	528	92	253	Probable glycosyl transferase Gly OS=Streptococcus gordonii OX=1302 GN=gly PE=3 SV=2
P27129	2.46e-11	280	514	30	272	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
A0A0H2URA3	2.69e-10	280	528	6	250	Glycosyltransferase GlyB OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003084_01015.