CAZyme Information: MGYG000003094_01043

Basic Information

• Species: Phocaeicola barnesiae
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola barnesiae
• CAZyme ID: MGYG000003094_01043
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
772	MGYG000003094_16|CGC1	88015.5	4.8032

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003094	3093382	MAG	Spain	Europe

• Gene Location: Start: 2900; End: 5218 Strand: +

Full Sequence      

MKKSLHHSTIITLLFLLLLSVTACKDTDELPDETPTVPDTDIAVVQDTAVKPTGGKASEC
QPGQDIDNTWDGKFSSDGGVPYHSVWNQSAAFPVTLEYYFEEGTEIDYLIYYTRSGNGNF
GELDLLIATDAEHSDWKPYGSYNFRMQNAPSKITFKETQKLTGIRFVVKSGAGNFVSCDE
MEFYRYNQQNDMEKQLLGVFSDITCSGLKEGVTEKEINALPEYFAALAKALRDNTYDSHE
REFRIRTYAPYSIAEEWAEKLMTRKYGNLDNPTGISVQEGDEIMVLVGDTHGQQISLQSI
WESGEEYRQTNVSGDIYFLRPGVNKLTMRNQGQLFVMYNTDLTAPEAQPVKIHILPGSGT
VTGFFDLKEHQTDEKYAELLSKATHKYFCVRGERIMFYFHTDKMREFVPTQILSAIHLWD
DIIGWEQELMGIEDVHPSQMNNHLFAISPEGSYMWATDYCIGFVHTYLNNILLKENVMAA
KDNAWGPAHEIGHIHQAAINWPGSTESSNNLFSNYVLYRLGKYCSRGSELDDLADSRFVY
HKAWYNMGDATHQGEDTEIHMRMNWQLWNYYHRCGHKKDFWQTLFKLLRADRIVESDPGA
AQLKFAMRASEAAGEDLTDFFDLWGFFIPGEDTIDQYGTWNYRVTEAMIQEAKDYMKQFP
KPKHAFQYIEDRKEGDTGLDITAPDTGYYTQFEGDVKPVSTAVCHTVSRRTYTVTNGENA
VAFELRKGGADGELVYFFNRFSYTIPEGVDLTGTGLFAVQADGKRIEVPRQK

Enzyme Prediction     

No EC number prediction in MGYG000003094_01043.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam18630	Peptidase_M60_C	2.31e-26	702	767	1	65	Peptidase M60 C-terminal domain. This is C-terminal domain (CTD) of M60-peptidases pfam13402. It Can also be found at the C-terminal region of gingipain B (RgpB) from P. gingivalis. It was found to possess a typical Ig-like fold encompassing seven antiparallel beta-strands organized in two beta-sheets, packed into a beta-sandwich structure that can spontaneously dimerize through C-terminal strand swapping. Translocation of gingipains from the periplasm across the OM is dependent on the conserved CTD, which appears to be important for secretion of the proteins and in particular, truncation of the last few C-terminal residues of this domain leads to accumulation of gingipains in the periplasm. Subsequently, the T9SS targeting signal was demonstrated to reside within the last 22 residues at the C-terminus of the CTD. During gingipain translocation across the OM, the CTD is cleaved off by PorU.
pfam13402	Peptidase_M60	5.59e-19	359	625	1	268	Peptidase M60, enhancin and enhancin-like. This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonisation of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.
pfam17291	M60-like_N	2.76e-05	272	353	22	107	N-terminal domain of M60-like peptidases. This accessory domain has a jelly roll topology.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAO79349.1	0.0	41	766	126	855
QQA07622.1	0.0	41	766	126	855
QUT42145.1	0.0	41	766	126	855
QUT69943.1	0.0	41	766	126	855
QUU10001.1	0.0	41	768	227	951

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5KD2_A	1.95e-285	192	766	27	605	BT_4244metallopeptidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
5KD5_A	2.35e-264	237	766	24	557	BT_4244metallopeptidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5KD8_A BT_4244 metallopeptidase in complex with Tn antigen. [Bacteroides thetaiotaomicron VPI-5482]
7SCI_A	2.80e-35	197	663	12	479	ChainA, Peptidase M60 domain-containing protein [Akkermansia muciniphila ATCC BAA-835]
7BLG_A	3.71e-20	47	184	22	160	ChainA, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLH_AAAA Chain AAAA, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLJ_A Chain A, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLK_A Chain A, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
5EV7_A	2.79e-16	272	625	45	379	Thecrystal structure of a functionally unknown conserved protein mutant from Bacillus anthracis str. Ames [Bacillus anthracis str. Ames]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000158	0.142604	0.857045	0.000067	0.000070	0.000057

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003094_01043.