CAZyme Information: MGYG000003099_01709

Basic Information

• Species: Barnesiella sp900538555
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Barnesiellaceae; Barnesiella; Barnesiella sp900538555
• CAZyme ID: MGYG000003099_01709
• CAZy Family: CBM6
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
965	MGYG000003099_10|CGC1	108593.95	4.5345

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003099	3270064	MAG	Spain	Europe

• Gene Location: Start: 90848; End: 93745 Strand: +

Full Sequence      

MKHRFIALLTMAACLFPLCGEAAARKFVHPGITYTQGDLDRMKAMVEAKVEPFYSTFLAL
KNSQYSDPNQPVTQRGTQIPEGRFNATVGIDGRRAIDLALLWHITGDRAYADKAVEFLNA
NSYYTNTSSRGTGPLDNGKVYLLIDAAELMRDYDGWAAEDQQRFKNMLTYPYYSTTEDLY
AKYASSDETKNGITFYWNCYNGDCARFGNQGLFAMRAIMSMGIYLDNDTIYDRAYRYLMA
MSHRPDDLAYPAGPPSVSSRPSSVGEYQDDYTLYGRDNTIEDYGYDELLKYYIYENGQCE
ESSRDQGHVMGGLHNYCQLAEMAWCQGDSLWTALDNRILKGLEWSYRYNLSFVKSYDDCP
EPWEPTGFTTDEKEATFENGLYYQTQVRAGRWALKNVSPAQRGDKAGYGGCREMALAQYA
VRQALDPEKYTWLERYRDYAIEKYGHEGWGESPNWYYEWTGWGTLSKRRTAWMAGDPVSF
ATGERVSGIHVIPAAIAAVDYDFYSYDNDGEGHTYHNVGTQRSSIYRTDGTVEIEQADGT
YVVTGIEAGEWMNYTVKVPVRGVYDVYVEYVAEAASTLTVAYDAATEVQGSLPASATFAK
AKIGSLPIEQAGAGVVRLICDAPAAGLKIKNIVIELSEGAKSVELTGSLDETTYTLNWSF
YGVLPQNVSVVRGYTDDPASATVLAENLSLSYYVDSDTDGKQPKTNYWLRYEDNGTVCYS
DPVCFEWGYFDDRFEDETETWMWNTEGMNYGQTAWGGNSLLVSYDSEKAYLKRVKTTVLH
GQFPILAFRMKVPQNTDIMLCSGTYKWNGTSNNYSRLEGLDDIYYYNLLDGNFRTSVNGS
PRYTVNNDEIVTLSALQLRYTVADASADPVRFYWARTFRTLDELGEYTASVGSVESVPFK
VGVDGRTLYFYELPEAAEITVYAIDGKSVVAGVRNMSSCELPAGGVYIVSLRCDRRCEVR
KVVVG

Enzyme Prediction     

No EC number prediction in MGYG000003099_01709.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04080	CBM6_cellulase-like	1.77e-12	492	566	1	73	Carbohydrate Binding Module 6 (CBM6); appended to glycoside hydrolase (GH) domains, including GH5 (cellulase). This family includes carbohydrate binding module 6 (CBM6) domains that are appended to several glycoside hydrolase (GH) domains, including GH5 (cellulase) and GH16, as well as to coagulation factor 5/8 carbohydrate-binding domains. CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. The CBM6s are appended to GHs that display a diversity of substrate specificities. For some members of this family information is available about the specific substrates of the appended GH domains. It includes the CBM domains of various enzymes involved in cell wall degradation including, an extracellular beta-1,3-glucanase from Lysobacter enzymogenes encoded by the gluC gene (its catalytic domain belongs to the GH16 family), the tandem CBM domains of Pseudomonas sp. PE2 beta-1,3(4)-glucanase A (its catalytic domain also belongs to GH16), and a family 6 CBM from Cellvibrio mixtus Endoglucanase 5A (CmCBM6) which binds to the beta1,4-beta1,3-mixed linked glucans lichenan, and barley beta-glucan, cello-oligosaccharides, insoluble forms of cellulose, the beta1,3-glucan laminarin, and xylooligosaccharides, and the CBM6 of Fibrobacter succinogenes S85 XynD xylanase, appended to a GH10 domain, and Cellvibrio japonicas Cel5G appended to a GH5 (cellulase) domain. GH5 (cellulase) family includes enzymes with several known activities such as endoglucanase, beta-mannanase, and xylanase, which are involved in the degradation of cellulose and xylans. GH16 family includes enzymes with lichenase, xyloglucan endotransglycosylase (XET), and beta-agarase activities. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. For CmCBM6 it has been shown that these two binding sites have different ligand specificities.
pfam16351	DUF4979	2.21e-12	728	884	1	158	Domain of unknown function (DUF4979). This family consists of uncharacterized proteins around 450 residues in length and is mainly found in various Bacteroides species. The function of this protein is unknown.
pfam05426	Alginate_lyase	3.18e-07	91	234	57	193	Alginate lyase. This family contains several bacterial alginate lyase proteins. Alginate is a family of 1-4-linked copolymers of beta -D-mannuronic acid (M) and alpha -L-guluronic acid (G). It is produced by brown algae and by some bacteria belonging to the genera Azotobacter and Pseudomonas. Alginate lyases catalyze the depolymerization of alginates by beta -elimination, generating a molecule containing 4-deoxy-L-erythro-hex-4-enepyranosyluronate at the nonreducing end. This family adopts an all alpha fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT82474.1	1.56e-283	21	639	22	641
QDM08619.1	1.56e-283	21	639	22	641
QGT69471.1	3.71e-283	21	635	22	637
QRQ56347.1	1.06e-282	21	635	22	637
SCV08998.1	1.06e-282	21	635	22	637

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000444	0.985261	0.013581	0.000255	0.000242	0.000215

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003099_01709.