CAZyme Information: MGYG000003114_00278

Basic Information

• Species: Clostridium sp900547475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900547475
• CAZyme ID: MGYG000003114_00278
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
777	MGYG000003114_2|CGC4	89824.77	6.2271

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003114	3467094	MAG	United States	North America

• Gene Location: Start: 83715; End: 86048 Strand: -

Full Sequence      

MIRDRSKFILKEDWKFHLGDEKEAFQKDFNDRDWESVYVPHDWSVTYPMSRNYSSGTGYV
RGGVGWYRKSFYLPKELKGKRVFIIFDGVYKNSQVWCNGYYMGKRPYGYSYFQYDISSQA
SFGDIENIISVKVSHEDIADSRWFTGSGIVRKVYIVVENEVYTKENGIFFTASNISKESA
EVKVENEIVFDKGKLGNIVIINNILDENEEVVAKFSKRDFIEEKVEKKIAIGGVINNPKL
WSVKKGYLYTLKTEVVCEDIGETYVIDEQKVGIRDIVFDKDKGFFLNGEHTTLKGVCLHD
DGGALGSAMTKDIWRRRLVKLKDMGCNAIRMSHNPHMPELYDLCDEMGFLVIDEAFDEWE
ASKNKWHIGHNVYPPKHEGYAEDFPEWHERDLTALVKKNRNHPSIIMWSIGNEIDYPNDP
YCHPLFNIMTGNNDKNKPDEEKKYNPNKPNMNRLSVIARDLSNIVKKLDLTRPVTAALAF
PELSTQIGYIDALDVVGYNYKEHLYRDDHKRFPDKPFLGSENGHSYDAWQAVIKNDFISG
QFLWTGIDYLGECAGWPYHGSQSGLLTTAGFEKPEYYRRKSFWSDKEMIHIVTARKGNDE
SEWKEMNESWNYITGEVIVVRCYTNVKNPQLFLNGILQSQFIDTSDKGYLTWNVVFEEGK
LEAIAGEVRHKLETVGAATTIRLKPISIEDIIQIELEILDCNNRNVYFDKSRIDIEVKGV
GKLVAIDNGDLRDVTDYTASYRDTNRGRLIIYVRRVGKGDIIIKARNPYLKEDRIIL

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	604	3.2e-115	0.6263297872340425

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.60e-67	3	552	8	505	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.16e-43	14	416	48	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	3.15e-42	14	415	19	419	beta-D-glucuronidase; Provisional
PRK09525	lacZ	9.02e-30	13	413	58	462	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	6.69e-27	10	155	4	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ99948.1	7.89e-304	14	777	9	809
QHQ63248.1	9.69e-300	4	754	2	763
CBL08200.1	1.82e-296	1	764	1	791
VCV23256.1	5.57e-296	1	764	1	791
CBL12712.1	5.74e-294	1	764	6	796

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T9A_A	3.50e-170	14	762	36	827	Crystalstructure of BuGH2Cwt [Bacteroides uniformis],5T9A_B Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_C Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_D Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9G_A Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_B Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_C Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_D Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis]
6B6L_A	5.17e-129	2	766	5	764	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	2.18e-128	2	766	5	764	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5T98_A	2.06e-120	10	771	30	812	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4YPJ_A	3.39e-118	10	773	12	801	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	7.86e-136	3	772	42	841	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	7.55e-98	14	740	55	779	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KN75	6.80e-51	4	777	25	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
T2KPJ7	3.44e-44	1	665	47	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.57e-43	1	410	1	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000046	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003114_00278.