CAZyme Information: MGYG000003114_02668

Basic Information

• Species: Clostridium sp900547475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900547475
• CAZyme ID: MGYG000003114_02668
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1359	MGYG000003114_37|CGC1	154524.3	4.3572

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003114	3467094	MAG	United States	North America

• Gene Location: Start: 12766; End: 16845 Strand: +

Full Sequence      

MKRLTSLILATTISIGLFNTLDIKVNAKENNTNIQLEENYEIYPLPQNETYLGSNFRITD
EVNIVVEDGIDESTRNFIIETLTANGINNIFSNEIVENKTNIIIGVRGSAGYVDNYFTEN
IDYNEKIFLEEDAYVLKVDNSLKENGVIAILGDTTDSAYYGIATLKMIFNQIENNQIKNV
MYEDFADAKWRGFIEGFYGYPWSNEDRKSLMEFGGNFKMNSYIFAPKDDPYHNRQWRELY
PDDKLAEIKELVDVGHKSKTQFIWAIHPGFNMIKWNDYDNELQTLLNKLEQLYSIGVRQF
GLFMDDISTDQSLTDKDWHVRLVTDVANWVKEKGDCDSLIYCPPYYNQAWTGEKGKPYLQ
ALSNVPKNVEIMWTGVDVCGQVVESEMQWPKDIIGRDIYMWLNWPVNGHNSSRLMLGKGE
VMDPGVHNISGIVTNPLEYAELSKTAIFAIADYTWNTDEFNDDKSWEDSFKYINPEVAEE
FHIIASHLSDPSPSNRNLVLEESEYIKEDLDLFLNNYNSGKDISEVGNRLIYEFDKILES
IVIFNSEKNLNEKMKSEIKPWLGSLEYIVKACKSSVNSAIAISESNLDVAWTELSNATVF
KQKSQTFTTKKLNSPDVVVESGAKRLIPFANELISSLDSKIYMQIDPQASSRIPISSYNS
NELDLIVDGDEETYAYFKTLQKVGDWYGIDLGKVIEVNNIEILQGRNDTDHDRFHKGILE
YSEDGESWVPIGEERTDSRIVENDLSIKARYIRYRATIAGVPGGKPDLWTAIREIRINKE
ENKPVLLTNVKELENINISISELKTEILNASNITLKTNEYLGFKLNEIEKIENLICEASS
QDITVETSINEIQWSEGTDTSEARYIRVINKRNDDITFDLNKLSVTLNKFQDTKITHNYG
GIYSGSIENVFDKKLESKVWFNSAQGKDKYVQVDLGGVVNIDKIDLVIGDSEKDYFRNGN
LEISLDGETWEVIDEITGESREANFPTLKAPYRYRTTEGINKEARYIRLISKNNNNAWLA
LNEILINDGINIDTNTNLSLIAKPEGELSNTSESIIDKKLSTFYTPAGETLDGELLYRLS
DYSEVGELIILQGPTGISNASVQIRDLDGWHEIGTLSNSYNYFDTSNLNNVLDVKLVWNG
IKPIINEIITVKKSSENTPNDIINKDELKSIIEKAKNIVENESDKYIEETIESLKEAITI
AEKVLDNDKATQDEVNEAYNTLIKEYLNLRLIPDKSLLEELIKELEALDLSKYTEKSANF
VKKELANASKVLSNEEATKTEIDEAVENLRRAKESLVEKDNSSNSNETESNDVESLSRDE
SNENLPKTGDIISSSVILIIAGVLIFGGVLIFKKKKILK

Enzyme Prediction     

No EC number prediction in MGYG000003114_02668.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	191	485	2.3e-103	0.9932203389830508

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	3.73e-132	191	482	1	291	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	9.93e-17	39	184	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam00754	F5_F8_type_C	9.17e-11	649	775	4	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	8.58e-08	649	753	10	125	Substituted updates: Jan 31, 2002
pfam00754	F5_F8_type_C	2.42e-07	904	1010	11	111	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASK62202.1	0.0	37	1162	38	1168
AIQ23051.1	0.0	2	1153	3	1162
APC49529.1	0.0	40	1156	34	1153
AIY84741.1	0.0	37	1299	42	1313
APC49528.1	0.0	65	1156	1	1096

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	2.25e-173	38	633	32	620	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	4.04e-131	37	646	29	653	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
2V5D_A	3.60e-81	44	757	18	714	Structureof a Family 84 Glycoside Hydrolase and a Family 32 Carbohydrate-Binding Module in Tandem from Clostridium perfringens. [Clostridium perfringens]
2J4G_A	7.74e-81	123	562	69	498	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2JIW_A	7.91e-81	123	562	70	499	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482],2JIW_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with 2- Acetylamino-2-deoxy-1-epivalienamine [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	2.37e-193	37	1218	36	1410	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q0TR53	1.08e-80	2	819	8	818	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	3.59e-80	2	832	8	825	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q89ZI2	6.73e-80	123	562	91	520	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
O60502	6.11e-31	192	461	63	337	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000315	0.998924	0.000232	0.000181	0.000166	0.000151

TMHMM  Annotations     

start	end
1330	1352