CAZyme Information: MGYG000003118_00955

Basic Information

• Species: Haemophilus_D parainfluenzae_J
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pasteurellaceae; Haemophilus_D; Haemophilus_D parainfluenzae_J
• CAZyme ID: MGYG000003118_00955
• CAZy Family: GT4
• CAZyme Description: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1043	MGYG000003118_6|CGC1	117687.55	8.6524

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003118	1969878	MAG	United States	North America

• Gene Location: Start: 1810; End: 4941 Strand: +

Full Sequence      

MTLFCSDISVSSTHDLAKISQKLCETLQNISSSTHNHQLSNYWLLSATDGITRAKTTSFQ
VLSLVETDITFHLEKLAQKFNAPLKWLRLEWVSSTQETTWEALQQELRKYKRNYFRKSIA
FKGKKYPWLLCSEMELNAHACFYNGADVGVAEVNKKNLQAYLKARHGSSEIPEFSDNMEI
LTFSTDGIFMDLSTKEIHRLSTTPRQHGRRELQPLDKNNLQPIIKNLTTYLGNQVKENGR
FEYGYFPVFGRTINTYNTLRHASSTYALIEGYEFCRTYQTGNLAKLKQQIEATLEYFIKS
FIRYYPNNLAYVIEINNEIKLGANAVAILALVKYIQVFPDSPNNKHYLTLAEALAEGIAF
MQQSDGSFVHILNATDLSIIAKNRVIYYDGEAAFGLMRLYNLTLNHRWLDIVMNAFNYFI
SAGHHKTHDHWLSYCSNELIQHCPAEKYFRFAVDNIKDYTHFIEKRITTFPTLLELSMAF
HKMLLKLDEFPQFSHVLDGFDVGQFYHALHTRANYLLNGIFFPEIAMFYKLPSSILWGAF
IRHHAFRVRIDDVEHYLSGLVAYHQLLSDNNYPKLAQKTGTDLVTPHCSLTAQGVAAATS
GKWIIPPPNDWLATGVAIHTLGFKPGSLLVARGKNMDKGFLTLPSVRSLISKGACGIITD
DSTHYLDFGVPVLQVQNVRQATLDLGAWARKHFSGRVIGITGSAGKTTTVAMMAAALKSS
KQVGHTIKSANLPIGTAWNMASLRSDLDYWVMEMAIGQMTLNSQITRPDIALITNIAPAH
LEYHDNVENIALKKARIFDAMKPGSYVIICRDIDQFDLIAEKAALRDLKVISYGESKNAD
MRLLSYEKGIATISINNEKLSLKLKTAGRHMALNAVAVLTVAYLEKFSLKEIVAALEEFE
AVDGRGNIFMSEIEGHRVTVYNEAYNANPLSMKAALEAFADNMINSDLKILVVGDMLELG
HNSEHLHRLIIERIALLPIKRIILVGKEMCKHAKWLETQDKIVLTAKDKIVATEHLAKIA
QTGDHVFLKASNGVGLHSLFKRI

Enzyme Prediction     

No EC number prediction in MGYG000003118_00955.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0770	MurF	6.30e-89	589	1036	2	441	UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis].
TIGR01143	murF	7.73e-85	624	1036	7	410	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11929	PRK11929	8.77e-61	596	1041	509	955	bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF.
PRK14093	PRK14093	4.82e-55	591	1033	8	454	UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase; Provisional
pfam08245	Mur_ligase_M	9.59e-36	700	882	1	200	Mur ligase middle domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWA95548.1	4.32e-122	43	565	29	546
QSI13415.1	4.32e-122	43	565	29	546
QYD19786.1	4.32e-122	43	565	29	546
BBK12984.1	4.32e-122	43	565	29	546
BBS46815.1	4.32e-122	43	565	29	546

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1GG4_A	8.54e-28	610	988	21	393	ChainA, UDP-N-ACETYLMURAMOYLALANYL-D-GLUTAMYL-2,6-DIAMINOPIMELATE-D-ALANYL-D-ALANYL LIGASE [Escherichia coli],1GG4_B Chain B, UDP-N-ACETYLMURAMOYLALANYL-D-GLUTAMYL-2,6-DIAMINOPIMELATE-D-ALANYL-D-ALANYL LIGASE [Escherichia coli]
4CVK_A	1.52e-27	609	968	21	380	PaMurFin complex with UDP-MurNAc-tripeptide (mDAP) [Pseudomonas aeruginosa PAO1],4CVL_A PaMurF in complex with AMP-PNP [Pseudomonas aeruginosa PAO1],4CVM_A PaMurF in complex with AMP-PNP and UDP-MurNAc-tripeptide (mDAP) [Pseudomonas aeruginosa PAO1]
4QDI_A	2.73e-26	591	987	21	408	ChainA, UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Acinetobacter baumannii AB307-0294]
4QF5_A	1.56e-25	591	987	21	408	ChainA, UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Acinetobacter baumannii AB307-0294],4QF5_B Chain B, UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Acinetobacter baumannii AB307-0294]
4ZIY_A	1.60e-25	591	987	23	410	ChainA, UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Acinetobacter baumannii AB5075]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9AKP1	4.41e-32	616	1039	25	444	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase OS=Rickettsia montanensis OX=33991 GN=murF PE=3 SV=1
Q92H60	8.00e-32	609	1039	18	444	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=murF PE=3 SV=1
O05953	6.38e-31	616	1039	25	444	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=murF PE=3 SV=2
P11880	1.73e-29	610	988	21	393	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase OS=Escherichia coli (strain K12) OX=83333 GN=murF PE=1 SV=2
P57315	6.52e-27	589	970	3	376	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=murF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000009	0.000037	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003118_00955.