CAZyme Information: MGYG000003123_01161

Basic Information

• Species: Chimaeribacter coloradensis
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Chimaeribacter; Chimaeribacter coloradensis
• CAZyme ID: MGYG000003123_01161
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
793	MGYG000003123_27|CGC1	85743.11	5.5534

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003123	3803778	MAG	United States	North America

• Gene Location: Start: 7188; End: 9569 Strand: +

Full Sequence      

MPCLYKDAAQPIAARVQDLLSRMTEEEKYAQMHAFWLILSPDGQHRSRRDLSDDFAGSAD
ADDLAARLALGIGQITRPLGTHIVEAKEGVRALNRLQKTLVEETRLGIPAMFHEECLVGL
LCKDATLFPSSLSYGATWDPELVEQVAAAIRAEAQSTGCHQGLAPVLDVSRDVRWGRTEE
TFGEDPYLVGKMATAYVRGLQGPKRDLLATLKHYVGHSFSEGARNHAPVHLGFRELNDTF
LLPFEMAVKLANAGSVMPAYHDIDNQPCHSDAFLLTEVLRNRWGFDGLIVADYGGVSLLH
QHHGVSADAAESAALAFNAGLDIELPKDDCARHLAEARQRGLISAEKIDEIVARILHEKF
RLGLFERPYADENAVSLQRETTRQLAWQAAVRAVTMLENNGILPLRPQAGQKIAVIGPTA
DDPLALLSGYSFPVHLIISDMAAQADVTTPLQALRDCYGEAAIHYAKGCHILEQRTAGAP
VFPGDSGTPLQHSPVSQDTHLIADAVAQAQACEVALVFVGDLAGLFQSGTVGEGSDTDSL
QLPGVQQQLLEAVVATGTPTIAILTGGRPYFLNGVEEKLAGYLAAFAPGQEGGRAIADVL
SGRELPSGRLVLSVPKSAGAMPYYYNHKLKSGGTPIAFSFGARYPFGYGLSGTTFHYGDL
QLAEASVPSRDGTVTAQLRITNSGDRPGSEVVQLYVKDRLASLVRPVHELKAFQRVELQP
GESAQLTFFLPTDMLNFTDAQGDRVVEPGEFELQAGASSADIRTRAVVTVTGSLHKLPSQ
WRMESHCEVAFPR

Enzyme Prediction     

EC	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	102	326	3.4e-67	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	7.83e-145	8	764	30	759	beta-glucosidase BglX.
COG1472	BglX	1.25e-81	23	423	1	367	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	5.62e-77	51	356	10	314	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	6.72e-59	105	756	78	769	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.17e-50	394	651	1	215	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCR38516.1	0.0	1	789	1	790
AVF34926.1	0.0	1	789	1	790
AEX51668.1	0.0	1	789	1	790
AFE57916.1	0.0	1	790	1	791
AZP41837.1	0.0	1	790	1	791

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5Z87_A	2.73e-133	5	770	36	785	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5Z9S_A	6.38e-120	5	759	17	775	Functionaland Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum],5Z9S_B Functional and Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum]
5XXL_A	2.72e-119	16	764	14	746	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.47e-118	16	764	14	746	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
6R5I_A	9.40e-109	16	766	7	729	ChainA, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KMH0	6.29e-112	105	764	62	717	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
P33363	1.13e-107	14	763	36	758	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH9	4.87e-107	5	763	29	748	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
Q56078	8.85e-106	14	763	36	758	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
A7LXU3	1.72e-76	12	762	35	768	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003123_01161.