CAZyme Information: MGYG000003123_02209

Basic Information

• Species: Chimaeribacter coloradensis
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Chimaeribacter; Chimaeribacter coloradensis
• CAZyme ID: MGYG000003123_02209
• CAZy Family: GH23
• CAZyme Description: Soluble lytic murein transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
642	MGYG000003123_83|CGC1	72471.47	9.5671

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003123	3803778	MAG	United States	North America

• Gene Location: Start: 7732; End: 9660 Strand: -

Full Sequence      

MVKVDKRRFLAVGIWLAIMAGSAHADSLDAQRQRYQQIKQAWDNKQMDTVAQLMPTLRDY
PLYPYLEYRQLTEDLGTAVSMHVTAFIKEHPTLPPARSLASRFVNELARREDWRGVLAFS
PEPPKPVAARCNYYYAKWATGQQQVAWDGAKAIWLTGNSLPASCDKLFTVWQQAGNVAPI
TILARIGLAMKEGNSNLVNFLAKQLPDDYQTMATALATLQNDPDSVESFARTVGPTDFTR
QAVTLAFERVAREDADNARSMLPVVARLQKMSDSEKLALEEAIAWRLMGSDVTAEQAAWR
DSVILRSHTTSLLERRVRMALGAGDQRGLATWLNRLPEEAQQKDEWRYWRASVLIDQGRR
SEGEAMLRSLMQARGFYPMVAAQKLNVPYTIRVEPSAAPDNRTAALPGIARVRELMYWGM
DNLARTEWSYLVASRNKTEQGQLARYAFNQGWADLSVQATITGKLWDNLQERFPMAWVQE
FRTNTEGKGITPSYAMAIARQESAWNPKAQSPVGAAGLMQVMPRTAEHTVKMSNIPGYSN
SSQLLDPQTNILIGTSYLEYVYQMLGRNRILSSAGYNAGPGRVTTWLENSAGRVDAVAFI
ESIPFSETRGYVKNVLSYDAFYRYFMHRPAKVLTDAEWQRRY

Enzyme Prediction     

No EC number prediction in MGYG000003123_02209.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	489	618	1.5e-28	0.7925925925925926

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11619	PRK11619	0.0	1	642	3	644	lytic murein transglycosylase; Provisional
cd13401	Slt70-like	2.56e-61	472	624	1	151	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
COG0741	MltE	5.99e-42	344	635	1	296	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
cd16896	LT_Slt70-like	4.02e-40	489	618	16	143	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	2.40e-33	481	593	1	109	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCR37928.1	0.0	4	642	1	639
VEA98287.1	0.0	4	642	1	639
CBY28708.1	0.0	4	642	1	639
VEF82916.1	0.0	4	642	1	639
QBP97907.1	0.0	4	642	1	639

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1QSA_A	3.19e-297	26	642	1	618	CrystalStructure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.65 Angstroms Resolution [Escherichia coli],1QTE_A Crystal Structure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.90 A Resolution In Complex With A 1,6- Anhydromurotripeptide [Escherichia coli]
1SLY_A	1.05e-295	26	642	1	618	ComplexOf The 70-Kda Soluble Lytic Transglycosylase With Bulgecin A [Escherichia coli]
5OHU_A	1.64e-91	22	642	21	638	TheX-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa [Pseudomonas aeruginosa]
6FBT_A	2.30e-89	31	642	1	609	ChainA, Lytic murein transglycosylase [Pseudomonas aeruginosa]
6FCQ_A	6.80e-89	31	642	1	609	ChainA, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCR_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCS_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCU_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0AGC3	1.29e-300	8	642	10	645	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P0AGC4	1.29e-300	8	642	10	645	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
P39434	8.59e-299	8	642	10	645	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P44888	4.45e-70	222	642	175	593	Putative soluble lytic murein transglycosylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=slt PE=3 SV=1
P39433	3.77e-14	601	642	1	42	Soluble lytic murein transglycosylase (Fragment) OS=Enterobacter cloacae OX=550 GN=slt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003419	0.986305	0.004899	0.004394	0.000607	0.000339

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003123_02209.