CAZyme Information: MGYG000003134_00573

Basic Information

• Species: Streptococcus mitis_BB
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus mitis_BB
• CAZyme ID: MGYG000003134_00573
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2184	MGYG000003134_4|CGC1	239917.64	5.2163

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003134	1850168	MAG	United States	North America

• Gene Location: Start: 57360; End: 63914 Strand: +

Full Sequence      

MDKRFFEKRCTYSIRKFALGAASVMIGASFFATAPVMANTPTVGSTDNLPSELADLDKKA
SDNGREFDKEAAAANSGSAETTDGPKTEEELLALEKKNKETTDKLPKELEGKAEKATDNG
KEVNKDQLAQDTGSLVPEDVAKTKNGELNYGATVKIKTPSGEGSGIVIGKDLVLTVSHNF
IKDQQDGNIRKVVDNDKGDGDIFSISYPDLDDVKFSKKDVIHWDRDGYLKGYKNDLALVR
LRTVLENAPVEVTEKPVVKKVGDKVNMFGYPAGKLAPVINSAVDFAESYGEGVQGVGYQG
GQPGASGGGIFDTDGKLIGVHQNGVVGVRSGGILFSPAQLKWIQDHIKGISSSKPANLEE
TEKPVEEKPKEDKPKEEKPATAKSETPKEVTPEWQTVARKEQQGTVAIREENGVRYNKLS
STDQNDNASKPALFEKQGLTVDANGNANVDLTFKEESETGKSRFGVFLKFKDTNNNVFVG
YDKSGWFWEYKTPGNSTWYQGGRVAAPVNGSVNHLTISLKSDGQLNATNNDVKLFDTVTL
PAAVNENLKNEKKILLKAGTYGTEKTVVNIKTDNQDGVQATEATAEKETGAVVDDSKVTY
DTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQLKKILVNNHEITPEVTYKKINDTTA
EYLMKLRDEKNFINSDMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSINFL
GNSLVSVSSDQAGAKFDGATMSNNTHISGDDHIAVTNPMKDLAKGYMYGFVSTDKLAAGV
WSNSQNSYGGGSNDWTRLTAFKQTVGNTNYVGIQSSEWQWEKAYKGIVFPEYTKELPSAK
VVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQNPFLMTLD
GIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKALIEKAKKYGAHL
GIHVNASETYPESKYFNENILRKNSDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEELK
NKLGEGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFQHWA
ADLTYGGYTNKGINSAITRFIRNHQKDSWVGDYRSYGGAANYPLLGGYSMKDFEGWQGRS
DYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMSDNGSTYKWTPEMRVELVDADNNKV
VVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLPADKEKMYYFNTQAG
ATTWTLPNDWAKNKVYLYKLTDQGKTEEQEVAVKDGKITLNLTANQPYVLYRSKQTNPEM
SWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANEMLRIQGNKSTVSLTQKLTGL
KPNTKYAVYVGVDNRSNAKASITVNTGEKEVTSYTNKSLALNYVKAYAHNTRRDNATVDD
TSYFQNMYAFFTTGSDVSNVTLTLSREAGDEATYFDEIRTFENNSSMYGDNHDTAKGTFK
QDFENVAQGIFPFVIGGIEGVEDNRTHLSEKHDPYTQRDWNGKKVDDVIEGNWSLKTNGL
VSRRNLVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGNQASNLEM
HELPNTWTDSKKAKKVTFLVTGADTGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFIMD
KLQIEEITLTGKMLTENALKNYLPTVAMTNYTKESMDALKEAVFNLSQADDEISVEEARA
EISKIDALKNALVQKKTALVAEDFESLNAPAQAGEDLANAFDGNLSSLWHTSWSGGDVGK
PATMVLKEATEITGFRYVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDID
FGKTIKAKKIVLTSTKTYGDGGDKYQAAAELIFSRPQVAETALDLSGYETALAKAQKLTS
KENQEEVASVVASMKYAADNHLLTERMVAYFAEYLNQLQDQTAKPDAPTSSKGEEVAPIL
EVPEYKGPVVTAGEEAVVNEIPEYKGGVNAAEAAVNEVPEYKGGANAVEALVHELPEYKG
GANAVEAAVNEVSEHKDGSNAAEVLVHELPEYKGGANAVEAAINEIPEYKGGANAVEALV
NEKPAYTGLLATAGDQAAPTVEKPEYQISQLGQGKLAESKTSVSTEEQKRLPETGESQSD
TAIFLAGVSLALSAAVLATKRKEN

Enzyme Prediction     

No EC number prediction in MGYG000003134_00573.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	605	1313	0	0.9985855728429985

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	1.42e-159	856	1146	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	7.66e-131	869	1169	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	2.73e-110	603	855	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17974	GalBD_like	3.60e-103	1476	1673	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam17995	GH101_N	2.17e-93	394	575	1	180	Endo-alpha-N-acetylgalactosaminidase N-terminal. This is the N-terminal domain found in Streptococcus pneumoniae endo-alpha-N-acetylgalactosaminidase (EC:3.2.1.97), a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. This is a twisted beta-sandwich domain composed of two sheets of six and seven antiparallel beta-strands. The domain appears to be missing the extended metal and carbohydrate-binding loops.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKL33722.1	0.0	1	2184	1	2204
AYF95093.1	0.0	1	2184	1	2187
QQQ35090.1	0.0	1	2184	1	2176
VEF78371.1	0.0	1	2184	1	2141
QQK99919.1	0.0	1	2184	1	2140

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QFK_A	0.0	596	1687	5	1116	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
5A59_A	0.0	586	1695	2	1111	Thestructure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen [Streptococcus pneumoniae TIGR4],5A5A_A The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with PNP-T-antigen [Streptococcus pneumoniae TIGR4]
6QEP_A	0.0	596	1687	5	1116	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	0.0	596	1687	5	1116	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	0.0	598	1891	22	1369	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	0.0	393	1974	124	1705	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	0.0	394	1974	125	1705	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	1.87e-114	602	1687	51	1037	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
P29767	8.46e-10	1771	1891	63	181	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000427	0.998812	0.000184	0.000218	0.000187	0.000151

TMHMM  Annotations     

start	end
17	39