CAZyme Information: MGYG000003134_00574

Basic Information

• Species: Streptococcus mitis_BB
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus mitis_BB
• CAZyme ID: MGYG000003134_00574
• CAZy Family: GH20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2813	MGYG000003134_4|CGC1	311602.35	5.4381

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003134	1850168	MAG	United States	North America

• Gene Location: Start: 64057; End: 72498 Strand: +

Full Sequence      

MDRHFFERRCHYSIRKFAIGAASVMIGASIFGANVVQAAETGGTPEKEGTITQAQPLDKL
PDDLAAVLKKAESEATADAGHEENHGNTVGTSPTATEETSSATSPTAPKPAETLKPSETP
KADSKPVEPATPTLKPVEKQIEDREDRNHLEGATAQANNYETGTPYTADKAVDGNPDTRW
ATNQNIEKPTFELTLPKTTLIRHVEIDWDRRVRNGQNDPNIKSWSLYYAGQDDVNASGEK
QWKLAHTKTGEPVLDEKVDLANSIQAKYLKLEINDYQAGTMGWRNVGIQEIRAYSNIPDT
SKVTDIRQVTELTVAKDGQSLVLPTLPGKVSLIGSNKQGVIDLQNHIYKPLTDQRVKVMV
EQIKDSHTFTKEFEVVIKGLHQDEGVGVKPKVAPAVQQWYGKEGQSSITSDTVLATGDSG
FDQAATFYQSDLASRGLELATGDKQAQKRIEFKKVENKGYGKEGYGLTIQDGVITIEAAT
NTGAFYATRTLLQMGESNLQNGEIRDFPSFSHRGFMLDTGRKFIPYDTLVDIMLNMAYYK
MNDLQLHLNDNYIFLKEHLAGKNLTQEEELKYVLEHAKTGFRVETDIVGKNGQKLTSDEH
YTKEEMQNLIKLAKALHINLVPEIDTPGHALSFVKVRPDLMYQGSLSDYRGKHNVERVAM
LDLDNKYDETLTFVKSVYDKLLDGPDAPLHGVSTVHIGTDEYYGSRESYRRYVNDLIKYI
KGKGYTPRIWGSLSAKRGKTAVDWKDVEVDIWSIGWQQPKEAIAQGAKIINITDIPTYSV
PNGDNRQAAYGDYANYETQYNGWTPNDFRTGHGPLLEASNPNIIGGGHAVWNDNIDLHET
GLTSYDIFKRFFKSMQTTAERTWGSDRAAATFAERILPASPYAPQSNPDRAINQSDLFTI
NPETVKNYASKKVKTSDQGLAFEKDSSIEGLAGDVGPSHVLKFDVTVTGDGEQTFSTSGD
NRIYLADKDGYLAYQFEQFHIQFKKKLEKNKRYKISIVTKPQSTEFYVDGEKIERIANPA
HPRFAHNSLVLPLESIGGFKGILHSAELSDKPFVNPRLIPTDHFTVSATSQETPGTATEG
TVEKAFDNDPNTFWHTKWTGDTAPYTLSMTLKEAEKVNGLTYLPRPGGGNGVVTRYEIYA
QKDGQMVKVSEGNWDNNTQEKTVNFAPVQTNKVELKVLAGVGGFASAAEVHLLKPIKEGQ
ETPAQPSTPETPKVEQTGDGTVELPDQFTASKPASEDSIAAASKSADYLKKEYKVFPTPQ
KVTYGEGVTALRKQVNLVMGDQLDVYTRNRLKSVLQDNQVSYTTSKSAVAGATNIYLGVH
GRGTQAEQNLSNVSAGLFDKIDAYALTIKDNSISIVGKDTDAVFYGLTTLKHMLKESQVP
VLRNVTVEDYAEIKNRGFIEGYYGNPWSNADRAELMRFGGDLKLTQYFFAPKDDPYHNKK
WRELYPEEKLAEIRELARVGNQSKTRYVWTIHPFMNNRIRFGNEAHYQEDLATIKAKFTQ
LMKVGVREFGILADDAPSPVGGYNSYNRLMQDMTNWLTEMQGTYSGLRKEMIFVPGQYWG
NGREAELKSLNENLPSSTSMTLTGGKIWGEVSEGFLSTLKNNLTAGGKTYRPVSLWVNWP
VTDNSKQHLILGGGEKFLHPNVDPSLLSGIMLNPMQQSEPSKIALFAGAQYTWKQWKSEE
EAKKLNDIAFNFVENGHFEDSKVSAAFRELGKHMINQNMDGRVVKLEESVELAPKLTDFM
TKLKAGQDVTAERVALRAEFAKIKEAAELYKASGDQKMVAQIHYWLDNAIDQMNALDAFL
TGTEAMTTNDAAKLWDSYYKGLKLYEQSQTHTFHYVDHLEKAELGVQHIRPFILSLKEVL
ASEVQKVLHPDKIISTFITNRTGVEGGLAEVTDGDLATHALIKSPSSIKTGDYIGMKFNK
PVDIQTLTFAMGTQANPRDTFSKAEVQYLDENDNWVTLKEPSYVGNESLLKFENLHIKAK
AVRMVATEDRENTWFAVQEIAVNRPVEKARGQQATTVSLSSNLVYKLNTSARQITDGKDN
TEAMMANADGSNTTPVDAWAQLDLGEVKSVTKVRLRQGTDDKLATGVLEYSTDGIAWQEL
DRLAGEQTKEVTRAINARYIRVRNTKASDIWWRIQDFSVETRSGNSDLTDTNVDALKETP
VVDSLGSYELQIPAGTKLPANSYLGMKLDRIHQVKSIQLQGQANPALSLEYSANAQEWTP
ASQLTDRTVATHLVRYVRLVNKTDQEQDLPSTSLLVTTKEVQPTKLESTTMGIHPTYGRN
DVRKINNLDQLFDGVYNNFVEFSDYAHKDGHVTLKLGSERTIKKIRAYIQDGTQNYLRDG
KIQVSQDGKTWTDVVTVGDGVANSTHDDSLTDGWTHDSKMPGNRYIEGELTTPVKANYLR
VLYTADYDARFVGFTELVINDGEFVKPINDPTVEGNGGESRGNFYTNLVDGKVLTSYKAE
NDKGELVYHLSEPTNANHLRLVSSLPEGVKARVQARTLKDGQESWTDLGAITSSLQTFAI
RNGGSLLDVKLVWEGGKAEFYELASFYQELTEEPVQSSKGEEPAPVLEVPEFTGGVNAVE
VLVHELPAYTGLVATVGDQAAPTVEQPEFKGGVNAVEALVNELPAYTGPVATVGDQVAPT
VEKPEFQGGVNSVMALKHELPEYTGQVSTVGDQPTPTVEKAEFKGGVNSVMALKHELPEY
TGPLSTVGDQPAPTVEKPEFQGGVNSVMALKHELPEYTGQLSTVGDQLAPTVEKPEFKLS
SLEKAQTPEAPVQVAKEDKRLPETGEKQSETAIFLASVGLALSAIFVAKSKKD

Enzyme Prediction     

EC	3.2.1.35	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	1396	1707	5e-95	0.9796610169491525
GH20	507	864	3.8e-62	0.9643916913946587
CBM32	1068	1191	2.7e-16	0.9516129032258065

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	5.43e-107	512	864	2	325	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam07555	NAGidase	5.79e-99	1396	1673	1	263	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
cd02742	GH20_hexosaminidase	3.50e-36	512	864	1	303	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	3.92e-31	510	863	1	343	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
cd06563	GH20_chitobiase-like	6.84e-28	510	863	1	342	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKL33721.1	0.0	1	2813	1	2815
AYF95094.1	0.0	1	2813	1	2778
QQQ35089.1	0.0	1	2813	1	2778
CBZ01293.1	0.0	1	2596	1	2601
QQK99918.1	0.0	1	2596	1	2601

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PV4_A	1.18e-186	1253	1861	32	645	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PWI_A	1.43e-104	1241	1860	22	624	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6JQF_A	3.19e-96	314	1041	12	724	Crystallizationanalysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835]
6PV5_A	2.25e-62	1234	1824	22	596	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
2V5D_A	1.06e-56	1257	1988	18	715	Structureof a Family 84 Glycoside Hydrolase and a Family 32 Carbohydrate-Binding Module in Tandem from Clostridium perfringens. [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	1.30e-287	1253	2558	39	1362	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
B2UPR7	1.46e-101	314	1041	34	746	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
Q8XL08	7.90e-55	1257	1988	48	745	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	1.05e-54	1257	1988	48	745	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q89ZI2	9.17e-52	1257	1786	28	520	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000477	0.998688	0.000197	0.000237	0.000204	0.000163

TMHMM  Annotations     

start	end
17	39