CAZyme Information: MGYG000003135_00106

Basic Information

• Species: Cutibacterium granulosum
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Cutibacterium; Cutibacterium granulosum
• CAZyme ID: MGYG000003135_00106
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
832	MGYG000003135_1|CGC1	89832.22	9.7727

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003135	2072889	MAG	United States	North America

• Gene Location: Start: 117166; End: 119664 Strand: +

Full Sequence      

MPSTHHRPASSNRSRARIDRLEAQTQSSSRFSAFLKFCLASILAGLLTAGFVVPFAALAS
TTARVSADSLQHLPAEFGSNPQPQRSQIFMADGSVMATFFDENREYVPLDKISPVMRQAQ
IAIEDNRFYSHGAIDLRGTMRALVSNMGGASTQGGSTLTQQYVKQARIEMAVSAGDEEGV
RSAQEQTVSRKLQELRYAIALEHDLSKDQILERYLNIAYYGDGAYGVEAAAKHYFGVTAD
KLDLGQAAMLAGLVQNPVSYDPVNNPEAAITRRNVVLTRMKGLGLVSEKDVQAAMKQGFN
RSKVTYPKTGCISSKYPFVCNYVYQSLLSSPNLGKTRAEREKLVKRGGLEIHTLIDPKAQ
DSAQEAVSNWVGPTDPVLGGVATIQPGTGLIMAMAQSRPEMGTKSGQTYYNYLAPLGLGG
FGGFQAGSTFKAYTLAAALEHGIPISKHYYAGSPMDFTGETFQGCKGKVRSGPFSVSNST
GHSGTMGLRAAAAYSVNTYFVQLERDAGLCNVTKMAKKTGVELASGKDIVKEYGSIPSFT
LGTAYVTPLSMASSYATFAARGVHCDPVIVKSIKNKDGQNLKVPDANCKRVMSTQVADGV
NEVLKGVMNGTGAPARIPGGYPQAGKTGTTDSNEAVWFTGYTPAVAGAAFIAADSSSEHF
RGREVRSIRGLHMSTGKYLKGSGGGDAGQIYRKAMAATLEDKPRKGFSETTDEIRHGKKV
PVPSTSGMNYDEAKKTLEDAGFDTRTKRVHSEKSKGTFLGADPDGGKAPQGSTIDLRVSS
GPRQTYQRRAPQRDRWQQWQQQQPGQQQQDPWQQQQQQPAPAEPPRYTQQPR

Enzyme Prediction     

No EC number prediction in MGYG000003135_00106.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	92	281	1.6e-68	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	4.98e-134	34	730	15	627	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	3.96e-123	103	701	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.55e-88	34	777	4	797	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	8.50e-82	92	281	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
TIGR02071	PBP_1b	2.73e-62	102	642	145	646	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SNV29356.1	0.0	1	832	1	833
QCV88022.1	0.0	28	782	6	759
VEI03701.1	0.0	28	782	6	759
AZZ38657.1	0.0	28	782	6	759
AZZ41174.1	0.0	28	782	6	759

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3DWK_A	1.54e-36	83	643	5	547	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
3UDF_A	3.29e-36	87	671	22	705	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	3.66e-35	201	660	3	440	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	4.34e-33	102	656	154	671	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.70e-33	102	656	175	692	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0R7G2	2.56e-61	76	712	101	697	Penicillin-binding protein 1A OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=ponA1 PE=3 SV=1
P39793	7.74e-59	83	650	74	611	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
P71707	8.22e-54	84	712	171	759	Penicillin-binding protein 1A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ponA1 PE=1 SV=3
P70997	3.32e-52	83	657	52	585	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
P38050	5.36e-48	86	659	49	576	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999221	0.000770	0.000002	0.000001	0.000000	0.000018

TMHMM  Annotations     

start	end
37	59